Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene

2004 ◽  
Vol 95 (3) ◽  
pp. 287-295 ◽  
Author(s):  
A.K. Knutsen ◽  
M. Torp ◽  
A. Holst-Jensen
MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.


Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.


2020 ◽  
Vol 70 (7) ◽  
pp. 4321-4328
Author(s):  
Cristina Torcato ◽  
Micael F. M. Gonçalves ◽  
Edgar Rodríguez-Gálvez ◽  
Artur Alves

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single- and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed.


2021 ◽  
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Chiung-Chih Chang

Abstract Three species of Aleurodiscus s.l. characterized in having effused basidiomata, clamped generative hyphae and quasi-binding hyphae, sulphuric positive reaction of gloeocystidia, hyphidia, acanthophyses and smooth basidiospores, are described. They are A. bicornis sp. nov., A. formosanus sp. nov. and A. parvisporus. Aleurodiscus bicornis was found from high mountains of NW Yunnan Province of SW China, grew on branch of Picea sp. Aleurodiscus formosanus was found from high mountains of central Taiwan, grew on branch of gymnosperm. Aleurodiscus parvisporus was previously reported only once from Japan and Sichuan Province of China respectively, and is reported in this study from Jilin Province of China. Phylogenetic relationships of these three species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1. The studied three species are phylogenetically closely related with significant support, corresponds with resemblance of their morphological features.


Phytotaxa ◽  
2019 ◽  
Vol 425 (5) ◽  
pp. 259-268
Author(s):  
XIAO-XIAO FENG ◽  
JIA-JIE CHEN ◽  
GUO-RONG WANG ◽  
TING-TING CAO ◽  
YONG-LI ZHENG ◽  
...  

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.


Phytotaxa ◽  
2020 ◽  
Vol 449 (2) ◽  
pp. 149-163
Author(s):  
DHANUSHKA N. WANASINGHE ◽  
PETER E. MORTIMER ◽  
CHANOKNED SENWANNA ◽  
RATCHADAWAN CHEEWANGKOON

During a survey of saprobic microfungi in Thailand, a dothideomycetous fungus was found on a dead twig of Delonix regia, on the Chiang Mai University campus. This fungus is characterized by fully immersed ascomata under a small blackened pseudoclypeus, pseudoparenchymatous peridium, cellular pseudoparaphyses, cylindrical-clavate asci with a distinct pedicel, overlapping 3–4-seriate, pale to dark brown, broadly fusoid, 7–9-transversally septate ascospores with a vertical septum in nearly all median cells. Multigene phylogenetic analyses, using partial sequences from the 28S nrRNA gene (LSU), 18S nrRNA gene (SSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon and the translation elongation factor 1-alpha region (TEF) demonstrated a monophyletic affiliation of the new strain, accommodating the species of Phaeoseptum in the family Phaeoseptaceae. With further morphological and phylogenetic investigations, we justify the new fungus as a novel species, Phaeoseptum hydei in Phaeoseptaceae. Detailed descriptions and illustrations are provided for Phaeoseptum hydei and this novel species compared with the remaining species found in the genus. An updated checklist of microfungi recorded on Delonix regia from around the world is also provided.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11435
Author(s):  
Jessa P. Ata ◽  
Kelly S. Burns ◽  
Suzanne Marchetti ◽  
Isabel A. Munck ◽  
Ludwig Beenken ◽  
...  

Increasing prevalence of conifer needle pathogens globally have prompted further studies on pathogen identification and a better understanding of phylogenetic relationships among needle pathogens. Several Lophodermella species can be aggressive pathogens causing needle cast in natural pine forests in the USA and Europe. However, their relationships with other Rhytismataceae species have historically been based on similarities of only limited phenotypic characters. Currently, no molecular studies have been completed to elucidate their relationships with other Lophodermella needle pathogens. This study collected and sequenced three gene loci, namely: internal transcribed spacer, large ribosomal subunit, and translation elongation factor 1-alpha, from five Lophodermella needle pathogens from North America (L. arcuata, L. concolor, L. montivaga) and Europe (L. conjuncta and L. sulcigena) to distinguish phylogeny within Rhytismatacaeae, including Lophophacidium dooksii. Phylogenetic analyses of the three loci revealed that all but L. conjuncta that were sampled in this study consistently clustered in a well-supported clade within Rhytismataceae. The multi-gene phylogeny also confirmed consistent nesting of L. dooksii, a needle pathogen of Pinus strobus, within the clade. Potential synapomorphic characters such as ascomata position and ascospore shape for the distinct clade were also explored. Further, a rhytismataceous species on P. flexilis that was morphologically identified as L. arcuata was found to be unique based on the sequences at the three loci. This study suggests a potential wider range of host species within the genus and the need for genetic characterization of other Lophodermella and Lophophacidium species to provide a higher phylogenetic resolution.


2020 ◽  
Vol 70 (5) ◽  
pp. 3433-3439
Author(s):  
Hua Zheng ◽  
Zefen Yu ◽  
Jianping Xu ◽  
Rafael F. Castañeda-Ruiz ◽  
Min Qiao

During a survey of endophytic fungi in aquatic plants collected from Tibet, PR China, a novel species, Ramichloridium endophyticum, was isolated from Potamogeton pectinatus. This novel species differs from other species of the genus Ramichloridium by its finely verrucose, obovoid, ellipsoidal–obovoid and occasionally subglobose conidia. Phylogenetic analysis of the combined sequences of the internal transcribed spacers (ITS) and the translation elongation factor 1-alpha gene (tef1-α) confirmed that the isolated strain represents a member of the genus Ramichloridium. A full description, illustrations and a phylogenetic tree showing the position of R. endophyticum are provided.


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