scholarly journals Molecular Components of Nitrogen Fixation Gene Cluster and Associated Enzymatic Activities of Non-Heterocystous Thermophilic Cyanobacterium Thermoleptolyngbya sp.

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 640
Author(s):  
Meijin Li ◽  
Lei Cheng ◽  
Jie Tang ◽  
Maurycy Daroch
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Microbial Mats ◽  
Hot Spring ◽  
Nitrogenase Activity ◽  
Second Phase ◽  
Two Phase ◽  
Fixation Capacity ◽  
Molecular Components ◽  
Nitrogen Fixation Gene

Thermoleptolyngbya is a genus of non-heterocystous cyanobacteria that are typical inhabitants of hot spring microbial mats. These filamentous cyanobacteria are capable of nitrogen fixation. In this study, we examined the genome sequences of five publicly available Thermoleptolyngbya strains to explore their nitrogen fixation gene cluster. Analysis of the nitrogen-fixation clusters in these extremophilic strains revealed that the cluster is located in a single locus in Thermoleptolyngbyace. The average nucleotide and amino acid identities of the nitrogen-fixation cluster combined with phylogenetic reconstructions support that nitrogen fixation genes in Thermoleptolyngbyaceae are closely related to one another but also heterogeneous within the genus. The strains from Asia, and China more specifically, generate a separate clade within the genus. Among these strains Thermoleptolyngbya sp. PKUAC-SCTB121 has been selected for experimental validation of clade’s nitrogen fixation capacity. The acetylene reduction experiments of that strain shown that the strain can reduce acetylene to ethylene, indicating a fully functional nitrogenase. The activity of nitrogenase has been tested using different gas compositions across 72 h and exhibited a two-phase trend, high nitrogenase activity at the beginning of the assay that slowed down in the second phase of the analysis.

scholarly journals Suppression ofhesAmutation on nitrogenase activity inPaenibacillus polymyxaWLY78 with the addition of high levels of molybdate or cystine

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6294 ◽  
Author(s):  
Xiaomeng Liu ◽  
Xiyun Zhao ◽  
Xiaohan Li ◽  
Sanfeng Chen
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Klebsiella Oxytoca ◽  
Paenibacillus Polymyxa ◽  
Gene Clusters ◽  
Nucleotide Binding Domain ◽  
Diazotrophic Cyanobacteria ◽  
Nitrogen Fixation Gene

The diazotrophicPaenibacillus polymyxaWLY78 possesses a minimal nitrogen fixation gene cluster consisting of nine genes (nifB nifH nifD nifK nifE nifN nifX hesAandnifV). Notably, thehesAgene contained within thenifgene cluster is also found withinnifgene clusters among diazotrophic cyanobacteria andFrankia. The predicted product HesA is a member of the ThiF-MoeB-HesA family containing an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. However, the function ofhesAgene in nitrogen fixation is unknown. In this study, we demonstrate that thehesAmutation ofP. polymyxaWLY78 leads to nearly complete loss of nitrogenase activity. The effect of the mutation can be partially suppressed by the addition of high levels of molybdate or cystine. However, the nitrogenase activity of thehesAmutant could not be restored byKlebsiella oxytoca nifQorEscherichia coli moeBcompletely. In addition, thehesAmutation does not affect nitrate reductase activity ofP. polymyxaWLY78. Our results demonstratehesAis a novel gene specially required for nitrogen fixation and its role is related to introduction of S and Mo into the FeMo-co of nitrogenase.

Sequences of nifX, nifW, nifZ, nifB and two ORF in the frankia nitrogen fixation gene cluster

Gene ◽  
1995 ◽  
Vol 161 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Olivia T. Harriott ◽  
Thomas J. Hosted ◽  
David R. Benson
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Nitrogen Fixation Gene

Evaluation of Biological Nitrogen Fixation Capacity in Arachis Species and the Possible Role of Polyploidy1

1994 ◽  
Vol 21 (1) ◽  
pp. 55-60 ◽  
Author(s):  
H. T. Stalker ◽  
M. L. Nickum ◽  
J. C. Wynne ◽  
G. H. Elkan ◽  
T. J. Schneeweis
Keyword(s):  
Nitrogen Fixation ◽  
Arachis Hypogaea ◽  
Cover Crops ◽  
Biological Nitrogen Fixation ◽  
Nitrogenase Activity ◽  
Diploid Species ◽  
Dry Matter Accumulation ◽  
Arachis Species ◽  
Fixation Capacity ◽  
Biological Nitrogen

Abstract Arachis species have potential for enhancing cultivated peanut (Arachis hypogaea L.) germplasm as forages and cover crops. This study's objective was to evaluate a range of Arachis species for biological nitrogen fixation capacity. Several Arachis species are tetraploids, and it has been shown that tetraploidy may play an important role in nodule initiation. Species were first tested under natural field conditions and then in the greenhouse using three Bradyrhizobium strains that had been previously shown to be effective on peanut. Nodule number, nodule weight, nitrogenase activity determined by acetylene reduction, and shoot dry weight were measured as indicators of nitrogen fixation capacity. In the field, tetraploid species produced significantly more nodules than the diploids, but total dry matter accumulation was independent of the number of nodules or rate of fixation. In the greenhouse, no significant differences were observed among the bradyrhizobial strains. Arachis hypogaea and A. monticola showed significantly higher measures of nitrogen fixation capacity for all measured traits than the diploid species. However, autotetraploid plants of A. villosa did not have significantly more nodules than diploids of the same accession; the autotetraploids consistently had higher nitrogenase activity. Arachis pusilla never formed a symbiotic relationship with the bradyrhizobial strains used.

scholarly journals Horizontal transfer of the nitrogen fixation gene cluster in the cyanobacterium Microcoleus chthonoplastes

2009 ◽  
Vol 4 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Henk Bolhuis ◽  
Ina Severin ◽  
Veronique Confurius-Guns ◽  
Ute I A Wollenzien ◽  
Lucas J Stal
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Horizontal Transfer ◽  
Microcoleus Chthonoplastes ◽  
Nitrogen Fixation Gene

scholarly journals Nitrogen Fixation by Paenibacillus Polymyxa WLY78 is Responsible for Cucumber Growth Promotion

2021 ◽  
Author(s):  
Shuang Liu ◽  
Qin Li ◽  
Yongbin Li ◽  
Tianyi Hao ◽  
Sanfeng Chen
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Gene Cluster ◽  
Isotope Dilution ◽  
Laser Scanning ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Confocal Laser ◽  
Greenhouse Experiments ◽  
Dilution Experiments

Abstract Aims To study nitrogen contribution to cucumber derived from nitrogen fixation of Paenibacillus polymyxa WLY78.Methods The nif gene cluster deletion mutant (ΔnifB-V) of P. polymyxa WLY78 is constructed by a homologous recombination method. The GFP-labeled ΔnifB-V mutant was used to inoculate cucumber and to study colonization by confocal laser scanning microscope. The effects of plant-growth promotion were investigated by greenhouse experiments. The nitrogen fixation contribution was estimated by 15N isotope dilution experiments. Results Deletion of nif gene cluster of P. polymyxa WLY78 resulted in complete loss of nitrogenase activity. Observation by laser confocal microscopy revealed ΔnifB-V mutant can effectively colonize cucumber root, stem and leaf tissues, like wild-type P. polymyxa WLY78. Greenhouse experiments showed that inoculation with P. polymyxa WLY78 can significantly enhance the lengths and dry weights of cucumber roots and shoots, but inoculation with ΔnifB-V mutant can not. 15N isotope dilution experiments showed that cucumber plants derive 25.93% nitrogen from nitrogen fixation performed by P. polymyxa WLY78, but the ΔnifB-V mutant nearly can not provide nitrogen for plant growth. Conclusions This present study demonstrates that nitrogen fixation performed by P. polymyxa WLY78 is responsible for cucumber growth promotion.

scholarly journals A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli

PLoS Genetics ◽  
2013 ◽  
Vol 9 (10) ◽  
pp. e1003865 ◽  
Author(s):  
Liying Wang ◽  
Lihong Zhang ◽  
Zhangzhi Liu ◽  
Dehua Zhao ◽  
Xiaomeng Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Paenibacillus Sp ◽  
Nitrogen Fixation Gene
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