scholarly journals The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells

Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1295
Author(s):  
Shingo Terashima ◽  
Hironori Yoshino ◽  
Yoshikazu Kuwahara ◽  
Hiro Sakuraba ◽  
Yoichiro Hosokawa
Keyword(s):  
Cell Survival ◽  
Dose Rate ◽  
High Dose Rate ◽  
High Dose ◽  
Dna Double Strand Breaks ◽  
Single Exposure ◽  
Strand Breaks ◽  
Cell Line Cell ◽  
Radiation Induced ◽  
Induced Apoptosis

We demonstrated that low dose pulsed radiation (0.25 Gy) at a high-dose-rate, even for very short intervals (10 s), decreases cell survival to a greater extent than single exposure to a similar total dose and dose rate. The objective of this study was to clarify whether high-dose-rate pulsed radiation is effective against SAS-R, a clinically relevant radioresistant cell line. Cell survival following high-dose-rate pulsed radiation was evaluated via a colony assay. Flow cytometry was utilized to evaluate γH2AX, a molecular marker of DNA double-strand breaks and delayed reactive oxygen species (ROS) associated with radiation-induced apoptosis. Increased cytotoxicity was observed in SAS-R and parent SAS cells in response to high dose rate pulsed radiation compared to single dose, as determined by colony assays. Residual γH2AX in both cells subjected to high-dose-rate pulsed radiation showed a tendency to increase, with a significant increase observed in SAS cells at 72 h. In addition, high-dose-rate pulsed radiation increased delayed ROS more than the single exposure did. These results indicate that high-dose-rate pulsed radiation was associated with residual γH2AX and delayed ROS, and high-dose-rate pulsed radiation may be used as an effective radiotherapy procedure against radioresistant cells.

The effect of high dose rate brachytherapy dwell sequence on cell survival

2001 ◽  
Vol 51 (3) ◽  
pp. 241-242
Author(s):  
M.R. Arnfield ◽  
P.S. Lin ◽  
M.A. Manning ◽  
D.W. Arthur ◽  
B.D. Kavanagh ◽  
...  
Keyword(s):  
Cell Survival ◽  
Dose Rate ◽  
High Dose Rate ◽  
High Dose ◽  
High Dose Rate Brachytherapy

Radiation-induced failures and degradation of wireless real-time dosimeter under high-dose-rate irradiation

2010 ◽  
Author(s):  
K. Tsuchiya ◽  
K. Kuroki ◽  
N. Akiba ◽  
K. Kurosawa ◽  
T. Matsumoto ◽  
...  
Keyword(s):  
Dose Rate ◽  
Real Time ◽  
High Dose Rate ◽  
High Dose ◽  
Radiation Induced

scholarly journals Assessing patient characteristics and radiation-induced non-targeted effects in vivo for high dose-rate (HDR) brachytherapy

2015 ◽  
Vol 91 (10) ◽  
pp. 786-794 ◽  
Author(s):  
Christine Pinho ◽  
Emilia Timotin ◽  
Raimond Wong ◽  
Ranjan K. Sur ◽  
Joseph E. Hayward ◽  
...  
Keyword(s):  
Dose Rate ◽  
Patient Characteristics ◽  
High Dose Rate ◽  
High Dose ◽  
Hdr Brachytherapy ◽  
Radiation Induced

Radiation-induced polymerization of methyl methacrylate and alkyl acrylates at high dose rate

1988 ◽  
Vol 36 (2) ◽  
pp. 295-308 ◽  
Author(s):  
Kanae Hayashi ◽  
Jun'ichi Takezaki ◽  
Toshio Okada ◽  
Ichiro Sakurada
Keyword(s):  
Methyl Methacrylate ◽  
Dose Rate ◽  
High Dose Rate ◽  
High Dose ◽  
Alkyl Acrylates ◽  
Radiation Induced

scholarly journals Comparable cell survival between high dose rate flattening filter free and conventional dose rate irradiation

2012 ◽  
Vol 52 (3) ◽  
pp. 652-657 ◽  
Author(s):  
Wilko F.A.R. Verbakel ◽  
Jaap van den Berg ◽  
Ben J. Slotman ◽  
Peter Sminia
Keyword(s):  
Cell Survival ◽  
Dose Rate ◽  
High Dose Rate ◽  
High Dose ◽  
Conventional Dose ◽  
Flattening Filter Free ◽  
Flattening Filter

Radiological Control of Gold Octahedral and Prism Nanoparticles

2007 ◽  
Vol 1056 ◽  
Author(s):  
Tina M. Nenoff ◽  
Jason C. Jones ◽  
Paula P. Provencio ◽  
Donald T. Berry
Keyword(s):  
Dose Rate ◽  
High Dose Rate ◽  
Pure Gold ◽  
High Dose ◽  
Visible Absorption ◽  
Dose Rates ◽  
Radiation Induced ◽  
Radiological Control ◽  
Uv Visible ◽  
Short Time

ABSTRACTWe report on a fundamental morphology growth of gold-based nanoparticles by solution radiolysis. Radiolysis of pure gold-polymer solutions of different dose rates and aging time is examined. A detailed description will be presented of the experimentation, testing and analysis. In particular, we will present data on the formation of gold nano-octahedra and -prism particles. The γ-irradiations were carried out with a 60Co source of 1.345 × 105 Ci (Sandia National Laboratories Gamma Irradiation Facility (GIF). Nanoparticle characterization techniques included are UV-vis and TEM. Similar to what has been seen in earlier silver nanoparticle studies, dose rate dictates the size of nanoparticles formed. At high dose rate, all reducing species are produced and scavenged within a short time, and then coalesce into separate nanoparticles. At low dose rate, the coalescence process is faster than the production rate of the reducing radicals. The reduction of radicals occurs mainly on clusters already formed. The differences in the morphologies result from a combination of dose rate, aging and lack of radical scavengers (e.g. isopropyl alcohol), resulting in either gold nano-spheres, octahedral or prism nanoparticles. The progressive evolution with dose rate of the UV-visible absorption spectra of radiation-induced metal clusters is discussed.

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