scholarly journals Photobiomodulation with Red and Near-Infrared Light Improves Viability and Modulates Expression of Mesenchymal and Apoptotic-Related Markers in Human Gingival Fibroblasts

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3427
Author(s):  
Ievgeniia Kocherova ◽  
Artur Bryja ◽  
Katarzyna Błochowiak ◽  
Mariusz Kaczmarek ◽  
Katarzyna Stefańska ◽  
...  

Photobiomodulation (PBM), also called low-level laser treatment (LLLT), has been considered a promising tool in periodontal treatment due to its anti-inflammatory and wound healing properties. However, photobiomodulation’s effectiveness depends on a combination of parameters, such as energy density, the duration and frequency of the irradiation sessions, and wavelength, which has been shown to play a key role in laser-tissue interaction. The objective of the study was to compare the in vitro effects of two different wavelengths—635 nm and 808 nm—on the human primary gingival fibroblasts in terms of viability, oxidative stress, inflammation markers, and specific gene expression during the four treatment sessions at power and energy density widely used in dental practice (100 mW, 4 J/cm2). PBM with both 635 and 808 nm at 4 J/cm2 increased the cell number, modulated extracellular oxidative stress and inflammation markers and decreased the susceptibility of human primary gingival fibroblasts to apoptosis through the downregulation of apoptotic-related genes (P53, CASP9, BAX). Moreover, modulation of mesenchymal markers expression (CD90, CD105) can reflect the possible changes in the differentiation status of irradiated fibroblasts. The most pronounced results were observed following the third irradiation session. They should be considered for the possible optimization of existing low-level laser irradiation protocols used in periodontal therapies.

2015 ◽  
Vol 08 (01) ◽  
pp. 1540002 ◽  
Author(s):  
Ting Li ◽  
Yue Zhao ◽  
Yunlong Sun ◽  
Kai Li

Low-level laser therapy (LLLT) has been clinically utilized for many indications in medicine requiring protection from cell/tissue death, stimulation of healing and repair of injuries, pain reduction, swelling and inflammation. Presently, the use of LLLT to treat stroke, traumatic brain injury and cognitive dysfunction are attracting growing interest. Near-infrared light is capable of penetrating into the cerebral cortex, allowing noninvasive treatments to be carried out with few treatment-related adverse events. Optimization of LLLT treatment effect is a crucial issue of this field; however, only a few experimental tests on mice for wavelength selection have been reported. We addressed this issue by low-cost, straightforward and quantitative comparisons on light dosage distribution within visible Chinese human head by Monte Carlo modeling of near-infrared light propagation. Optimized selection in wavelength, beam type and size were given based on comparisons among frequently used setups (i.e., wavelengths: 660, 810 and 980 nm; beam type: Gaussian and flat beam; beam diameter: 2, 4 and 6 cm). This study provided an efficient way for guiding the optimization of LLLT setup and selection on wavelength, beam type and size for clinical brain LLLT.


2012 ◽  
Author(s):  
Ying-Ying Huang ◽  
Clark E. Tedford ◽  
Thomas McCarthy ◽  
Michael R. Hamblin

2020 ◽  
Vol 24 (1) ◽  
pp. 9-17
Author(s):  
V. I. Kozlov ◽  
V. V. Astashov

The authors present a review of publications in the scientific and medical literature on modern concepts of mechanisms of low-level laser biostimulating effects at the human body, at microvasculature chains and lymphoid organs. The main aspect of microcirculation photoactivation is the process of microcirculation reconstruction which leads to prolonged improvement in tissue trophism. There are three processes in the basement of biostimulating effect of low-level laser light in the red and near infrared spectral regions at microcirculation: hemomicrocirculation enhancement, activation of capillary neovascularization, lymphocorrection effects at the structure and functional activity of lymphoid organs.


2021 ◽  
Vol Volume 14 ◽  
pp. 1045-1051
Author(s):  
Anna Szymańska ◽  
Elzbieta Budzisz ◽  
Anna Erkiert-Polguj

2006 ◽  
Vol 36 (3) ◽  
pp. 667-676
Author(s):  
Hoda Hamdy ◽  
Mamdouh El-Yamany ◽  
Mohamed Shetewy ◽  
Afaf El-Faras ◽  
Aziza Ibrahim

2012 ◽  
Vol 89 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Flávia Mafra de Lima ◽  
Regiane Albertini ◽  
Yvana Dantas ◽  
Antonio Luis Maia-Filho ◽  
Cristiano de Loura Santana ◽  
...  

2014 ◽  
Vol 29 (6) ◽  
pp. 1867-1874 ◽  
Author(s):  
Razvan-Alexandru Campeanu ◽  
Beatrice Mihaela Radu ◽  
Sanda Maria Cretoiu ◽  
Daniel Dumitru Banciu ◽  
Adela Banciu ◽  
...  

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