Evaluation of the in Vitro Anti-Atherogenic Properties of Lipid Fractions of Olive Pomace, Olive Pomace Enriched Fish Feed and Gilthead Sea Bream (Sparus aurata) Fed with Olive Pomace Enriched Fish Feed

Abstract Receptors for GH were characterized in the head kidney of gilthead sea bream (Sparus aurata), using radioiodinated and biotinylated ligands. The specific binding of radiolabelled recombinant gilthead sea bream GH (rsbGH) to head kidney membrane preparations was dependent on membrane concentration. Salmon prolactin, salmon gonadotrophin and carp gonadotrophin did not compete for 125I-labelled rsbGH-binding sites. Unlabelled rsbGH competitively displaced 125I-labelled rsbGH bound to head kidney membranes. Scatchard plots were always linear, denoting the presence of a single class of binding sites. The binding affinity (Ka=2·7 × 109 m−1) was equivalent to that found in liver membrane preparations, but the binding capacity (2·5 ±0·30 fmol/mg protein) was 50- to 75-fold lower. To identify the cells which express the GH receptor, head kidney smears were incubated with biotinylated rsbGH, followed by incubation with an avidin–biotin complex conjugated to alkaline phosphatase. The reaction with the new-fuchsin substrate gave a red precipitate, showing a specific and intense labelling in erythroblasts, polychromatophilic erythroblasts and myeloblasts. Noticeable binding was observed in myelocytes and immature granulocytes, tending to disappear at the latter stages of granulocyte maturation. Light but appreciable binding was also observed in monocytes, lymphocytes and acidophilic erythroblasts, whereas it was completely absent in proerythrocytes and erythrocytes. The proliferative action of rsbGH and recombinant human IGF-I on in vitro cultures of head kidney cells was demonstrated by a 5-bromo-2′-deoxy-uridine immunoassay. To our knowledge, this is the first report that provides suitable evidence for a role of GH as a haemopoietic growth and differentiation factor in lower vertebrate species. Journal of Endocrinology (1995) 146, 459–467

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Isolation of Growth Hormone and in Vitro Translation of mRNA Isolated from Pituitaries of the Gilthead Sea Bream Sparus aurata

General and Comparative Endocrinology ◽

10.1006/gcen.1994.1129 ◽

1994 ◽

Vol 95 (3) ◽

pp. 321-329 ◽

Cited By ~ 5

Author(s):

B. Cavari ◽

P.-Y. Le Bail ◽

B. Levavi-Sivan ◽

P. Melamed ◽

H. Kawauchi ◽

...

Keyword(s):

Growth Hormone ◽

Sparus Aurata ◽

In Vitro Translation ◽

Gilthead Sea Bream ◽

Sea Bream

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In vivo and in vitro treatments against Sparicotyle chrysophrii (Monogenea: Microcotylidae) parasitizing the gills of gilthead sea bream (Sparus aurata L.)

Aquaculture ◽

10.1016/j.aquaculture.2006.09.012 ◽

2006 ◽

Vol 261 (3) ◽

pp. 856-864 ◽

Cited By ~ 44

Author(s):

Ariadna Sitjà-Bobadilla ◽

Magnolia Conde de Felipe ◽

Pilar Alvarez-Pellitero

Keyword(s):

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream

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Structural and functional characterizations of activin type 2B receptor (acvr2b) ortholog from the marine fish, gilthead sea bream, Sparus aurata: evidence for gene duplication of acvr2b in fish

Journal of Molecular Endocrinology ◽

10.1530/jme-12-0075 ◽

2012 ◽

Vol 49 (3) ◽

pp. 175-192 ◽

Cited By ~ 4

Author(s):

Bruria Funkenstein ◽

Ekaterina Krol ◽

Elena Esterin ◽

Yong-soo Kim

Keyword(s):

Transforming Growth Factor ◽

Sparus Aurata ◽

Fish Larvae ◽

Larval Growth ◽

Amino Acid Sequences ◽

Negative Regulator ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Luciferase Assay

Myostatin (MSTN), a negative regulator of muscle growth and a member of the transforming growth factor-β superfamily, can bind the two activin type 2 receptors (ACVR2). It has been previously shown that WT mice injected with ACVR2B extracellular domain (ACVR2B-ECD) had higher muscle mass. Likewise, fish larvae immersed inPichia pastorisculture supernatant, containing goldfish Acvr2b-ECD, showed enhanced larval growth. However, it is not clear whether fish Mstn1 and Mstn2 signal through the same receptor and whether fish express more than oneacvr2bgene. In the current study, three cDNAs encodingacvr2b(saacvr2b-1, saacvr2b-2a, and saacvr2b-2b) were cloned from gilthead sea bream. All three contain the short extracellular binding domain, a short transmembrane region, and a conserved catalytic domain of serine/threonine protein kinase. Bioinformatics analysis provided evidence for the existence of twoacvr2bgenes (acvr2b-1 andacvr2b-2) in several other fish species as well, probably as a result of gene or genome duplication. The two isoforms differ in their amino acid sequences. The direct inhibitory effect of Acvr2b-ECD on Mstn activity was testedin vitro. The saAcvr2b-1-ECD was expressed in the yeastP. pastoris. Evidence is provided for N-glycosylation of Acvr2b-1-ECD. The affinity-purified Acvr2b-1-ECD inhibited recombinant mouse/rat/human mature MSTN activity when determinedin vitrousing the CAGA-luciferase assay in A204 cells. A lower inhibitory activity was obtained when unprocessed purified, furin-digested, and activated saMstn1 was used. Results of this study demonstrate for the first time the existence of twoacvr2bgenes in fish. In addition, the study shows that bioactive fish Acvr2b-ECD can be produced fromP. pastoris.

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In vitro characterization of acid secretion in the gilthead sea bream (Sparus aurata) stomach

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpa.2013.10.016 ◽

2014 ◽

Vol 167 ◽

pp. 52-58 ◽

Cited By ~ 6

Author(s):

Lorenzo Márquez ◽

Juan Fuentes

Keyword(s):

Acid Secretion ◽

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

In Vitro Characterization

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Comparison of Sensory and Cardioprotective Properties of Olive-Pomace Enriched and Conventional Gilthead Sea Bream (Sparus aurata): The Effect of Grilling

Journal of Aquatic Food Product Technology ◽

10.1080/10498850.2013.813100 ◽

2014 ◽

Vol 24 (8) ◽

pp. 782-795 ◽

Cited By ~ 12

Author(s):

Eleni Sioriki ◽

Constantina Nasopoulou ◽

Constantinos A. Demopoulos ◽

Ioannis Zabetakis

Keyword(s):

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Olive Pomace

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Pharmacokinetics of chloramphenicol (CAP) in gilthead sea bream (Sparus aurata) and its in vitro activities against important bacterial fish pathogens

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15340 ◽

2017 ◽

Vol 54 (4) ◽

pp. 311 ◽

Cited By ~ 2

Author(s):

A. E. TYRPENOU (Α.Ε. ΤΥΡΠΕΝΟΥ) ◽

G. RIGOS (Γ. ΡΗΓΟΣ) ◽

M. YAGNISI (Μ. ΓΙΑΓΝΙΣΗ) ◽

I. NENGAS (Ι. ΝΕΓΚΑΣ) ◽

M. ALEXIS (Μ. ΑΛΕΞΗΣ)

Keyword(s):

Half Life ◽

Sparus Aurata ◽

Vibrio Anguillarum ◽

Central Compartment ◽

Apparent Volume ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Farmed Fish ◽

Fish Pathogens

The pharmacokinetics of chloramphenicol (CAP) in gilthead sea bream (Sparus aurata), a warm water farmed fish species and its in vitro efficacy against important bacterial diseases of Mediterranean mariculture were investigated in this study. After an intravascular injection (10 mg/kg/fish), the distribution half-life (tl/2a) and the elimination half-life (tl/2ß) of CAP were calculated to be 1.6 and 69 h, respectively. Tissue penetration of CAP was found to be moderate since both the apparent volume of distribution of the drug at steady-state (V<i(ss)) and the apparent volume of the central compartment (Vc) were calculated to be 1.13 and 0.90 L/kg. The total clearance (CLp) of the drug was slow (0.022 L/kg/h). The minimum inhibitory concentration (MIC) values of CAP in distilled water supplemented with 2% NaCl against Vibrio anguillarum serotype lb, Photobacterium damsela subsp. piscicida, V alginolyticus and V ßuvialis were determined to be 4.78 pg/mL, while it was 0.60 pg/mL for V. damsela. The addition of 10 mM Ca2+ and 55 mM Mg2+ in the medium revealed MIC values of 19.13 pg/mL for V alginolyticus and V ßuvialis, whereas showed no effect for V anguillarum P. damsela subsp. Piscicida and V damsela. The results indicate that CAP displayed a satisfactory kinetic profile and it is eliminated fast from gilthead sea bream muscle; however, its high MIC values stress show a possible inefficacy against important bacterial pathogens of Mediterranean mariculture.

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