scholarly journals Pharmacokinetics of chloramphenicol (CAP) in gilthead sea bream (Sparus aurata) and its in vitro activities against important bacterial fish pathogens

2017 ◽  
Vol 54 (4) ◽  
pp. 311 ◽  
Author(s):  
A. E. TYRPENOU (Α.Ε. ΤΥΡΠΕΝΟΥ) ◽  
G. RIGOS (Γ. ΡΗΓΟΣ) ◽  
M. YAGNISI (Μ. ΓΙΑΓΝΙΣΗ) ◽  
I. NENGAS (Ι. ΝΕΓΚΑΣ) ◽  
M. ALEXIS (Μ. ΑΛΕΞΗΣ)
Keyword(s):  
Half Life ◽  
Sparus Aurata ◽  
Vibrio Anguillarum ◽  
Central Compartment ◽  
Apparent Volume ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Farmed Fish ◽  
Fish Pathogens

The pharmacokinetics of chloramphenicol (CAP) in gilthead sea bream (Sparus aurata), a warm water farmed fish species and its in vitro efficacy against important bacterial diseases of Mediterranean mariculture were investigated in this study. After an intravascular injection (10 mg/kg/fish), the distribution half-life (tl/2a) and the elimination half-life (tl/2ß) of CAP were calculated to be 1.6 and 69 h, respectively. Tissue penetration of CAP was found to be moderate since both the apparent volume of distribution of the drug at steady-state (V<i(ss)) and the apparent volume of the central compartment (Vc) were calculated to be 1.13 and 0.90 L/kg. The total clearance (CLp) of the drug was slow (0.022 L/kg/h). The minimum inhibitory concentration (MIC) values of CAP in distilled water supplemented with 2% NaCl against Vibrio anguillarum serotype lb, Photobacterium damsela subsp. piscicida, V alginolyticus and V ßuvialis were determined to be 4.78 pg/mL, while it was 0.60 pg/mL for V. damsela. The addition of 10 mM Ca2+ and 55 mM Mg2+ in the medium revealed MIC values of 19.13 pg/mL for V alginolyticus and V ßuvialis, whereas showed no effect for V anguillarum P. damsela subsp. Piscicida and V damsela. The results indicate that CAP displayed a satisfactory kinetic profile and it is eliminated fast from gilthead sea bream muscle; however, its high MIC values stress show a possible inefficacy against important bacterial pathogens of Mediterranean mariculture.

scholarly journals Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 362
Author(s):  
Amparo Picard-Sánchez ◽  
M. Carla Piazzon ◽  
Itziar Estensoro ◽  
Raquel Del Pozo ◽  
Nahla Hossameldin Ahmed ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Horizontal Transmission ◽  
Infected Fish ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Target Tissue ◽  
Farmed Fish ◽  
Infection Level ◽  
Available Information

Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.

Growth hormone as an in vitro phagocyte-activating factor in the gilthead sea bream ( Sparus aurata )

1997 ◽  
Vol 287 (3) ◽  
pp. 535-540 ◽  
Author(s):  
Josep Alvar Calduch-Giner ◽  
Ariadna Sitjà-Bobadilla ◽  
Pilar Alvarez-Pellitero ◽  
Jaume Pérez-Sánchez
Keyword(s):  
Growth Hormone ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream

Corrigendum to “In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans” [Aquaculture 416–417 (2013) 185–191]

Aquaculture ◽  
2015 ◽  
Vol 442 ◽  
pp. 138
Author(s):  
George Rigos ◽  
Evdokia Karagouni ◽  
Ioannis Kyriazis ◽  
Evita Athanasiou ◽  
Kriton Grigorakis ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
In Vivo Evaluation ◽  
Cryptocaryon Irritans

Evidence for a direct action of GH on haemopoietic cells of a marine fish, the gilthead sea bream (Sparus aurata)

1995 ◽  
Vol 146 (3) ◽  
pp. 459-467 ◽  
Author(s):  
J A Calduch-Giner ◽  
A Sitjà-Bobadilla ◽  
P Álvarez-Pellitero ◽  
J Pérez-Sánchez
Keyword(s):  
Binding Sites ◽  
Sparus Aurata ◽  
Binding Capacity ◽  
Specific Binding ◽  
Head Kidney ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Single Class ◽  
Lower Vertebrate

Abstract Receptors for GH were characterized in the head kidney of gilthead sea bream (Sparus aurata), using radioiodinated and biotinylated ligands. The specific binding of radiolabelled recombinant gilthead sea bream GH (rsbGH) to head kidney membrane preparations was dependent on membrane concentration. Salmon prolactin, salmon gonadotrophin and carp gonadotrophin did not compete for 125I-labelled rsbGH-binding sites. Unlabelled rsbGH competitively displaced 125I-labelled rsbGH bound to head kidney membranes. Scatchard plots were always linear, denoting the presence of a single class of binding sites. The binding affinity (Ka=2·7 × 109 m−1) was equivalent to that found in liver membrane preparations, but the binding capacity (2·5 ±0·30 fmol/mg protein) was 50- to 75-fold lower. To identify the cells which express the GH receptor, head kidney smears were incubated with biotinylated rsbGH, followed by incubation with an avidin–biotin complex conjugated to alkaline phosphatase. The reaction with the new-fuchsin substrate gave a red precipitate, showing a specific and intense labelling in erythroblasts, polychromatophilic erythroblasts and myeloblasts. Noticeable binding was observed in myelocytes and immature granulocytes, tending to disappear at the latter stages of granulocyte maturation. Light but appreciable binding was also observed in monocytes, lymphocytes and acidophilic erythroblasts, whereas it was completely absent in proerythrocytes and erythrocytes. The proliferative action of rsbGH and recombinant human IGF-I on in vitro cultures of head kidney cells was demonstrated by a 5-bromo-2′-deoxy-uridine immunoassay. To our knowledge, this is the first report that provides suitable evidence for a role of GH as a haemopoietic growth and differentiation factor in lower vertebrate species. Journal of Endocrinology (1995) 146, 459–467

scholarly journals Isolation of Growth Hormone and in Vitro Translation of mRNA Isolated from Pituitaries of the Gilthead Sea Bream Sparus aurata

1994 ◽  
Vol 95 (3) ◽  
pp. 321-329 ◽  
Author(s):  
B. Cavari ◽  
P.-Y. Le Bail ◽  
B. Levavi-Sivan ◽  
P. Melamed ◽  
H. Kawauchi ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Sparus Aurata ◽  
In Vitro Translation ◽  
Gilthead Sea Bream ◽  
Sea Bream

scholarly journals Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2920 ◽  
Author(s):  
Ruben Gil-Solsona ◽  
Jaime Nácher-Mestre ◽  
Leticia Lacalle-Bergeron ◽  
Juan Vicente Sancho ◽  
Josep Alvar Calduch-Giner ◽  
...  
Keyword(s):  
High Performance ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Adaptive Responses ◽  
Farmed Fish ◽  
Tandem Mass Spectra ◽  
Flight Mass Spectrometry ◽  
Clear Differentiation ◽  
Variance Explained

A metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. More than 15,000 differentm/zions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitine-related metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish.

In vivo and in vitro treatments against Sparicotyle chrysophrii (Monogenea: Microcotylidae) parasitizing the gills of gilthead sea bream (Sparus aurata L.)

Aquaculture ◽  
2006 ◽  
Vol 261 (3) ◽  
pp. 856-864 ◽  
Author(s):  
Ariadna Sitjà-Bobadilla ◽  
Magnolia Conde de Felipe ◽  
Pilar Alvarez-Pellitero
Keyword(s):  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream

scholarly journals Evaluation of the in Vitro Anti-Atherogenic Properties of Lipid Fractions of Olive Pomace, Olive Pomace Enriched Fish Feed and Gilthead Sea Bream (Sparus aurata) Fed with Olive Pomace Enriched Fish Feed

Marine Drugs ◽  
2013 ◽  
Vol 11 (10) ◽  
pp. 3676-3688 ◽  
Author(s):  
Constantina Nasopoulou ◽  
Vassiliki Gogaki ◽  
Giorgos Stamatakis ◽  
Leonidas Papaharisis ◽  
Constantinos Demopoulos ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Fish Feed ◽  
Olive Pomace ◽  
Lipid Fractions

scholarly journals Structural and functional characterizations of activin type 2B receptor (acvr2b) ortholog from the marine fish, gilthead sea bream, Sparus aurata: evidence for gene duplication of acvr2b in fish

2012 ◽  
Vol 49 (3) ◽  
pp. 175-192 ◽  
Author(s):  
Bruria Funkenstein ◽  
Ekaterina Krol ◽  
Elena Esterin ◽  
Yong-soo Kim
Keyword(s):  
Transforming Growth Factor ◽  
Sparus Aurata ◽  
Fish Larvae ◽  
Larval Growth ◽  
Amino Acid Sequences ◽  
Negative Regulator ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Luciferase Assay

Myostatin (MSTN), a negative regulator of muscle growth and a member of the transforming growth factor-β superfamily, can bind the two activin type 2 receptors (ACVR2). It has been previously shown that WT mice injected with ACVR2B extracellular domain (ACVR2B-ECD) had higher muscle mass. Likewise, fish larvae immersed inPichia pastorisculture supernatant, containing goldfish Acvr2b-ECD, showed enhanced larval growth. However, it is not clear whether fish Mstn1 and Mstn2 signal through the same receptor and whether fish express more than oneacvr2bgene. In the current study, three cDNAs encodingacvr2b(saacvr2b-1, saacvr2b-2a, and saacvr2b-2b) were cloned from gilthead sea bream. All three contain the short extracellular binding domain, a short transmembrane region, and a conserved catalytic domain of serine/threonine protein kinase. Bioinformatics analysis provided evidence for the existence of twoacvr2bgenes (acvr2b-1 andacvr2b-2) in several other fish species as well, probably as a result of gene or genome duplication. The two isoforms differ in their amino acid sequences. The direct inhibitory effect of Acvr2b-ECD on Mstn activity was testedin vitro. The saAcvr2b-1-ECD was expressed in the yeastP. pastoris. Evidence is provided for N-glycosylation of Acvr2b-1-ECD. The affinity-purified Acvr2b-1-ECD inhibited recombinant mouse/rat/human mature MSTN activity when determinedin vitrousing the CAGA-luciferase assay in A204 cells. A lower inhibitory activity was obtained when unprocessed purified, furin-digested, and activated saMstn1 was used. Results of this study demonstrate for the first time the existence of twoacvr2bgenes in fish. In addition, the study shows that bioactive fish Acvr2b-ECD can be produced fromP. pastoris.

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