scholarly journals Anti-Allergic Effect of Low Molecular Weight Digest from Abalone Viscera on Atopic Dermatitis-Induced NC/Nga

Marine Drugs ◽  
2021 ◽  
Vol 19 (11) ◽  
pp. 634
Author(s):  
Tae-Hee Kim ◽  
Seong-Yeong Heo ◽  
Gun-Woo Oh ◽  
Won Sun Park ◽  
Il-Whan Choi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Atopic Dermatitis ◽  
Histological Analysis ◽  
Immunoglobulin E ◽  
Clinical Symptoms ◽  
Low Molecular Weight ◽  
Dermatophagoides Farinae ◽  
Promising Candidate ◽  
Seafood Processing ◽  
Allergic Effect

Abalone viscera (AV) is one of the byproducts of the seafood processing industry. The low molecular weight (<5 kDa) peptides (LMW-AV) obtained from gastrointestinal digestion of AV could suppress allergenic responses on activated HMC-1 human mast cells in our previous study. Regarding the allergenic response of LMW-AV, in the present study, we further investigated the potential of oral administration of LMW-AV against atopic dermatitis (AD) in a dermatitis-induced model stimulated with Dermatophagoides farinae. The results demonstrated that the LMW-AV reduced a number of clinical symptoms, such as the severity of the dermatitis and serum immunoglobulin E levels. Moreover, LMW-AV could inhibit the expression of chemokines and cytokines. The histological analysis indicated that the LMW-AV has suppressed the eosinophil count and the mast cell infiltration into the upper dermis. The results suggest that LMW-AV can be considered as a promising candidate for AD treatment.

scholarly journals Metabolomics Distinguishes DOCK8 Deficiency from Atopic Dermatitis: Towards a Biomarker Discovery

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 274 ◽  
Author(s):  
Minnie Jacob ◽  
Xinyun Gu ◽  
Xian Luo ◽  
Hamoud Al-Mousa ◽  
Rand Arnaout ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Biomarker Discovery ◽  
Isotope Labeling ◽  
Immunoglobulin E ◽  
Clinical Symptoms ◽  
Elevated Serum ◽  
Severe Atopic Dermatitis ◽  
Serum Ige ◽  
Tryptophan Degradation ◽  
Dock8 Deficiency

Bi-allelic mutations in the dedicator of cytokinesis 8 (DOCK8) are responsible for a rare autosomal recessive primary combined immunodeficiency syndrome, characterized by atopic dermatitis, elevated serum Immunoglobulin E (IgE) levels, recurrent severe cutaneous viral infections, autoimmunity, and predisposition to malignancy. The molecular link between DOCK8 deficiency and atopic skin inflammation remains unknown. Severe atopic dermatitis (AD) and DOCK8 deficiency share some clinical symptoms, including eczema, eosinophilia, and increased serum IgE levels. Increased serum IgE levels are characteristic of, but not specific to allergic diseases. Herein, we aimed to study the metabolomic profiles of DOCK8-deficient and AD patients for potential disease-specific biomarkers using chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS). Serum samples were collected from DOCK8-deficient (n = 10) and AD (n = 9) patients. Metabolomics profiling using CIL LC-MS was performed on patient samples and compared to unrelated healthy controls (n = 33). Seven metabolites were positively identified, distinguishing DOCK8-deficient from AD patients. Aspartic acid and 3-hydroxyanthranillic acid (3HAA, a tryptophan degradation pathway intermediate) were up-regulated in DOCK8 deficiency, whereas hypotaurine, leucyl-phenylalanine, glycyl-phenylalanine, and guanosine were down-regulated. Hypotaurine, 3-hydroxyanthranillic acid, and glycyl-phenylalanine were identified as potential biomarkers specific to DOCK8 deficiency. Aspartate availability has been recently implicated as a limiting metabolite for tumour growth and 3HAA; furthermore, other tryptophan metabolism pathway-related molecules have been considered as potential novel targets for cancer therapy. Taken together, perturbations in tryptophan degradation and increased availability of aspartate suggest a link of DOCK8 deficiency to oncogenesis. Additionally, perturbations in taurine and dipeptides metabolism suggest altered antixidation and cell signaling states in DOCK8 deficiency. Further studies examining the mechanisms underlying these observations are necessary.

scholarly journals Low Molecular Weight Fraction of Dermatophagoides Farinae is Present in Sera of Patients with Bronchial Asthma.

2001 ◽  
Vol 51 (4) ◽  
pp. 255-259
Author(s):  
Akihiro Morikawa ◽  
Shozo Maeda ◽  
Makoto Shigeta ◽  
Hirokazu Arakawa ◽  
Hiroshi Tamura ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bronchial Asthma ◽  
Weight Fraction ◽  
Low Molecular Weight ◽  
Dermatophagoides Farinae

Immunoglobulin-E Reactivity and Structural Analysis of Wheat Low-Molecular-Weight Glutenin Subunits and Their Repetitive and Nonrepetitive Halves

2012 ◽  
Vol 60 (30) ◽  
pp. 7538-7547 ◽  
Author(s):  
Hamza Mameri ◽  
Jacques Snégaroff ◽  
Yann Gohon ◽  
Catherine Pecquet ◽  
Dominique Choudat ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Structural Analysis ◽  
Immunoglobulin E ◽  
Low Molecular Weight ◽  
Glutenin Subunits

Anti-allergic effect of low molecular weight β-glucan prepared by λ-irradiation

2011 ◽  
Vol 20 (3) ◽  
pp. 841-844 ◽  
Author(s):  
Nak-Yun Sung ◽  
Jong-il Choi ◽  
Yohan Yoon ◽  
Soo-Young Lee ◽  
Myung-Woo Byun ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Allergic Effect

Children with atopic dermatitis and frequent emollient use have increased urinary levels of low-molecular-weight phthalate metabolites and parabens

Allergy ◽  
2017 ◽  
Vol 72 (11) ◽  
pp. 1768-1777 ◽  
Author(s):  
L. E. K. Overgaard ◽  
K. M. Main ◽  
H. Frederiksen ◽  
S. Stender ◽  
P. B. Szecsi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Atopic Dermatitis ◽  
Low Molecular Weight ◽  
Phthalate Metabolites ◽  
Urinary Levels

Inhibition of allergic airway responses by inhaled low-molecular-weight heparins: molecular-weight dependence

1998 ◽  
Vol 84 (1) ◽  
pp. 222-228 ◽  
Author(s):  
José Martinez-Salas ◽  
Richard Mendelssohn ◽  
William M. Abraham ◽  
Bernard Hsiao ◽  
Tahir Ahmed
Keyword(s):  
Molecular Weight ◽  
Airway Hyperresponsiveness ◽  
Immunoglobulin E ◽  
Low Molecular Weight ◽  
Low Molecular Weight Heparins ◽  
Antiallergic Activity ◽  
Effective Fraction ◽  
Molecular Weight Dependence ◽  
Lung Resistance ◽  
Airway Responses

Martinez-Salas, José, Richard Mendelssohn, William M. Abraham, Bernard Hsiao, and Tahir Ahmed. Inhibition of allergic airway responses by inhaled low-molecular-weight heparins: molecular-weight dependence. J. Appl. Physiol. 84(1): 222–228, 1998.—Inhaled heparin prevents antigen-induced bronchoconstriction and inhibits anti-immunoglobulin E-mediated mast cell degranulation. We hypothesized that the antiallergic action of heparin may be molecular weight dependent. Therefore, we studied the effects of three different low-molecular-weight fractions of heparin [medium-, low-, and ultralow-molecular-weight heparin (MMWH, LMWH, ULMWH, respectively)] on the antigen-induced acute bronchoconstrictor response (ABR) and airway hyperresponsiveness (AHR) in allergic sheep. Specific lung resistance was measured in 22 sheep before and after airway challenge with Ascaris suum antigen, without and after pretreatment with inhaled fractionated heparins at doses of 0.31–5.0 mg/kg. Airway responsiveness was estimated before and 2 h postantigen as the cumulative provocating dose of carbachol in breath units that increased specific lung resistance by 400%. All fractionated heparins caused a dose-dependent inhibition of ABR and AHR. ULMWH was the most effective fraction, with the inhibitory dose causing 50% protection (ID50) against ABR of 0.5 mg/kg, whereas ID50values of LMWH and MMWH were 1.25 and 1.8 mg/kg, respectively. ULMWH was also the most effective fraction in attenuating AHR; the ID50values for ULMWH, LMWH, and MMWH were 0.5, 2.5, and 4.7 mg/kg, respectively. These data suggest that 1) fractionated low-molecular-weight heparins attenuate antigen-induced ABR and AHR; 2) there is an inverse relationship between the antiallergic activity of heparin fractions and molecular weight; and 3) ULMWH is the most effective fraction preventing allergic bronchoconstriction and airway hyperresponsiveness.

Successful Attenuation of Venous Thrombus Growth in Rabbits after the Administration of a Novel Oral Thrombin Inhibitor

2000 ◽  
Vol 84 (11) ◽  
pp. 858-864 ◽  
Author(s):  
Tymen Keller ◽  
Bart Biemond ◽  
Ron Peters ◽  
Wilfried Hornberger ◽  
Harry Büller ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Bleeding Time ◽  
Direct Thrombin Inhibitor ◽  
Thrombin Inhibitor ◽  
Low Molecular Weight ◽  
Antithrombotic Effect ◽  
Promising Candidate ◽  
Venous Thrombus ◽  
Saline Administration

SummaryCurrent antithrombotic compounds have several limitations in clinical practice. The present study was designed to investigate a novel orally available direct thrombin inhibitor, BSF 208791. Intravenous administration of BSF 208791 showed superior antithrombotic properties as compared with Polyethylenglycol-Hirudin (PEG-Hirudin) and low molecular weight heparin (LMWH) in a model of venous thrombosis in rabbits. The thrombus growth was 22%, 30%, 37% and 50% after BSF 208791, PEG-Hirudin, LMWH, and saline administration, respectively. Moreover, bleeding time was less affected after administration of BSF 208791 as compared with PEG-Hirudin. The oral administration of BSF 208791 resulted in adequate bioavailability and significantly reduced venous thrombus growth to 36% as compared with 60% in the saline treated rabbits. The antithrombotic effect of BSF 208791 appears to be superior to PEG-Hirudin and LMWH without affecting the bleeding time. BSF 208791 is an orally available agent that might be a promising candidate for future antithrombotic therapy.

scholarly journals Topical Application ofChrysanthemum indicum L.Attenuates the Development of Atopic Dermatitis-Like Skin Lesions by Suppressing Serum IgE Levels, IFN-γ, and IL-4 in Nc/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Sunmin Park ◽  
Jung Bok Lee ◽  
Suna Kang
Keyword(s):  
Atopic Dermatitis ◽  
Topical Application ◽  
Immunoglobulin E ◽  
Clinical Symptoms ◽  
Secondary Infection ◽  
Skin Lesions ◽  
Mrna Levels ◽  
Dorsal Skin ◽  
Gene Expressions ◽  
Serum Ige

Chrysanthemum indicum L. (CIL) is widely used as an anti-inflammatory agent in Asia and our preliminary study revealed that CIL reduced interleukin (IL)-4 and IL-13 in 2,4-dinitrochlorobenzene (DNCB)-treated HaCaT cells, a human keratinocyte cell line. We investigated the atopic dermatitis (AD) effect of topically applied CIL in mice with AD-like symptoms. After topical application of 1,3-butylen glycol (control), CIL-Low (5%), CIL-High (30%), or 0.1% hydrocortisone (HC) on the AD-like skin lesions in DNCB-treated NC/Nga mice for 5 weeks, the ear thickness, mast cell infiltration, and serum immunoglobulin E (IgE), IgG1, IL-4 and interferon (IFN)-γwere measured. The gene expressions of IL-4, IL-13, and IFN-γin the dorsal skin were assayed. CIL treatment dosedependently reduced severity of clinical symptoms of dorsal skin, ear thickness, and the number of mast cells and eosinophils. CIL-High significantly decreased serum IgE, IgG1, IL-4, and IFN-γlevels and reduced mRNA levels of IFN-γ, IL-4, and IL-13 in dorsal skin lesion. The improvement by CIL-High was similar to HC, but without its adverse effects such as skin atrophy maceration, and secondary infection. In conclusion, CIL may be an effective alternative substance for the management of AD.

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