scholarly journals Activity of Neutrophil β-Glucuronidase in Diabetic and Nondiabetic Patients With Chronic Generalized Periodontitis and Healthy Subjects

Medicina ◽  
2011 ◽  
Vol 47 (2) ◽  
pp. 12 ◽  
Author(s):  
Algimantas Šurna ◽  
Jurgina Sakalauskienė ◽  
Alvydas Gleiznys ◽  
Eglė Ivanauskienė ◽  
Viktoras Šaferis
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Type 1 Diabetes ◽  
Healthy Subjects ◽  
Venous Blood ◽  
Periodontal Tissue ◽  
Opsonized Zymosan ◽  
Glucuronidase Activity ◽  
Neutrophilic Leukocytes

Objective. The aim of the study was to establish the dynamics of β-glucuronidase activity in subjects suffering from type 1 diabetes and chronic untreated generalized periodontitis, subjects suffering from chronic untreated generalized periodontitis only, and control subjects not suffering from generic diseases with healthy periodontal tissue. Material and Methods. The study involved 165 19–50-year-old subjects who were divided into three groups: healthy subjects (n=55), subjects with chronic untreated generalized periodontitis (n=55), and subjects with type 1 diabetes and chronic untreated generalized periodontitis (n=55). Neutrophilic leukocytes of peripheral venous blood were exposed to bacterial stimuli: opsonized zymosan, nonopsonized Staphylococcus aureus, and prodigiosan. The activity of β-glucuronidase was determined by the spectrofluorimetry method. Results. The diagnostic value of changes in β-glucuronidase activity of neutrophilic leukocytes markedly increased in all study groups after stimulation of neutrophilic leukocytes by opsonized zymosan, nonopsonized Staphylococcus aureus, and prodigiosan as compared to control media not exposed to any stimulus (P<0.001). The strongest relationship (canonical correlation coefficient eta, 0.993) between the intensity of periodontal pathology markers and the activity of β-glucuronidase of neutrophilic leukocytes in incubated media in patients with type 1 diabetes mellitus and periodontitis was found under the effect of nonopsonized Staphylococcus aureus. Conclusions. If periodontal impairment is severe, diabetes mellitus possibly causes a faster destruction of the periodontal tissue and presents a higher risk of periodontitis for patients with diabetes.

scholarly journals Association of the CCR5 gene with type 1 diabetes mellitus

2013 ◽  
Vol 59 (2) ◽  
pp. 3-6
Author(s):  
O I Kopylova ◽  
T L Kuraeva ◽  
E Iu Lavrikova ◽  
E V Titovich ◽  
A G Nikitin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Healthy Subjects ◽  
Significant Rise ◽  
Diabetic Patients ◽  
Ccr5 Gene ◽  
Base Pair Deletion ◽  
Time Amplification

Aim of the study. To elucidate the association between the polymorphous marker A/del132 of the CCR5 gene with type 1 diabetes mellitus. Materials and methods. The study included 177 patients with type 1 diabetes mellitus (DM1) and 408 healthy subjects (ethnic Russians). CR5 alleles and genotypes were identified with the use of the real-time amplification technique. Results. It was shown that the CCR5 allele without 32 base pair deletion (allele A) predominated in both diabetic patients and diabetes-free subjects. The difference between their occurrence in the two groups was insignificant. At the same time, we documented a significant rise in the frequency of del132/del132 genotype in the diabetic patients compared with the healthy subjects (p=0.008). It is concluded that carriers of the CCR5-del32/del32 genotype in the Russian population of Moscow are at high risk of developing type 1 diabetes mellitus.

scholarly journals The vasomotor function of endothelium and elasticity of the arterial wall in the patients with type 1 diabetes mellitus at different stages of diabetic retinopathy

2012 ◽  
Vol 58 (4) ◽  
pp. 22-26
Author(s):  
E P Kosobian ◽  
I Ia Iarek-Martynova ◽  
A S Parfenov ◽  
M V Shestakova
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Diabetic Retinopathy ◽  
Type 1 Diabetes Mellitus ◽  
Healthy Subjects ◽  
Reactive Hyperemia ◽  
Signal Amplitude ◽  
Stiffness Index ◽  
Retinal Damage

The objective of the present work was to estimate the degree of rigidity of the vascular wall and endothelial function in the patients with type 1 diabetes mellitus at different stages of diabetic retinopathy and in its absence. The study included 118 patients at the age from 18 to 40 years presenting with type 1 diabetes mellitus more than 5 years in duration. The patients were divided into several groups based on the stage of diabetic retinopathy (DR). The control group was comprised of 23 healthy subjects. The contour pulsed wave analysis and the reactive hyperemia test were performed with the use of an Angioscan device. All the patients gave the informed consent to participate in the study. The aortic stiffness index (SI) in all groups of the patents with DM1 and DR was higher than in the healthy subjects. However, the SI values remained normal in the DM1 patients without DR. An increase of SI values was unrelated to the presence of arterial hypertension. The analysis of RI values revealed no statistically significant differences between the groups. The reactive hyperemia test has demonstrated a decrease in the increment of the post-occlusive signal amplitude in a large number of patients suggesting endothelial dysfunction. This decrease was apparent even in the patients exhibiting no signs of retinal damage. However, despite the overall decrease of this parameter, many patients showed its paradoxically high values that probably reflected the elevated baseline level of nitric oxide. The increase of aortic stiffness index with the progression of diabetic retinopathy may be used as an early marker of macrovascular complications even in the patients without arterial hypertension. The lowering of the increment of the post-occlusive signal amplitude in the patients having no signs of retinal damage is indicative of endothelial dysfunction as early as the preclinical stage of DR development. The paradoxically high increment of the signal amplitude is supposed to be associated with the elevation of the baseline level of nitric oxide that in its turn reflects the severity of inflammation and is a factor of high risk of progression of angiopathies.

scholarly journals Differences in plasma apelin and visfatin levels between patients with type 1 diabetes mellitus and healthy subjects and response after acute hyperglycemia and insulin administration

HORMONES ◽  
2012 ◽  
Vol 11 (4) ◽  
pp. 444-450 ◽  
Author(s):  
Kleopatra Alexiadou ◽  
Alexander Kokkinos ◽  
Stavros Liatis ◽  
Despoina Perrea ◽  
Nicholas Katsilambros ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Healthy Subjects ◽  
Insulin Administration ◽  
Acute Hyperglycemia ◽  
Plasma Apelin

scholarly journals Studying Studying the relationship between Dipeptidyl peptidase-4 enzyme level, Tumor necrosis factor alpha enzyme level, and insulin resistance in type 1 Diabetes Mellitus patients

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
H M Amer ◽  
M M M Mahdy ◽  
W E Saad ◽  
M N A Abdelsalam
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Serum Level ◽  
Type 1 Diabetes Mellitus ◽  
Enzyme Level ◽  
Venous Blood ◽  
P Value ◽  
Tnf Α ◽  
The Relationship

Abstract Background Type 1 diabetes mellitus (T1DM) is an inflammatory disease of the pancreatic islets that results in absolute insulin deficiency and the consecutive hyperglycemia due to immune-mediated beta cell destruction throughout the globe, the incidence of T1DM is increasing at 3% to 5% per year Purpose To study the relationship between DPP-4 serum level, TNF a concentration measurements, and insulin sensitivity in T1DM patient Patients and Methods Patients were selected from the inpatient department of National Institute of Diabetes and Endocrinology during Januaray 2017, venous blood samples were collected to measure Serum level of creatinine, C-peptide, HbA1c%, DPP-4, TNFα . Measurement of eGDR was done using the formula 24.31 - 12.2 x (WHR) – 3.29 x (BLP) – 0.57 X HbA1c Study Design Cross sectional study that included 80 patients with type 1 Diabetes, Results The study population was divided according to median eGDR value of 6.125 mg kg-1 min-1 The group with higher IR(eGDR≤ 6.125)had higher value of DPP-4 enzyme level (1127.3 ± 40.9 ng/ml vs. 870.6 ± 30.2 ng/ml, p &lt; 0.001) and TNF α concentration (11.4 ± 4.8 pg/ml vs 5.3 ± 1.6 pg/ml, p &lt; 0.001). TNF α concentration negatively correlated with eGDR (r= -0.515, p value &lt;0.001) DPP-4 level negatively correlated with eGDR (r= -0.826 and p value &lt;0.001) while DPP-4 was positively correlated with TNF α (r = 0.441 p value ≤ 0.001)

scholarly journals Thrombopoietin Contributes to Enhanced Platelet Activation in Patients with Type 1 Diabetes Mellitus

2021 ◽  
Vol 22 (13) ◽  
pp. 7032
Author(s):  
Ornella Bosco ◽  
Barbara Vizio ◽  
Gabriella Gruden ◽  
Martina Schiavello ◽  
Bartolomeo Lorenzati ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Platelet Activation ◽  
Healthy Subjects ◽  
Diabetic Patients ◽  
Potential Contribution ◽  
Atherosclerotic Cardiovascular Disease

Atherosclerotic cardiovascular disease is the major cause of morbidity and mortality in patients with type 1 diabetes mellitus (T1DM). Enhanced platelet reactivity is considered a main determinant of the increased atherothrombotic risk of diabetic patients. Thrombopoietin (THPO), a humoral growth factor able to stimulate megakaryocyte proliferation and differentiation, also modulates the response of mature platelets by enhancing both activation and binding to leukocytes in response to different agonists. Increased THPO levels have been reported in different clinical conditions characterized by a generalized pro-thrombotic state, from acute coronary syndromes to sepsis/septic shock, and associated with elevated indices of platelet activation. To investigate the potential contribution of elevated THPO levels in platelet activation in T1DM patients, we studied 28 T1DM patients and 28 healthy subjects. We measured plasma levels of THPO, as well as platelet-leukocyte binding, P-selectin, and THPO receptor (THPOR) platelet expression. The priming activity of plasma from diabetic patients or healthy subjects on platelet–leukocyte binding and the role of THPO on this effect was also studied in vitro. T1DM patients had higher circulating THPO levels and increased platelet–monocyte and platelet–granulocyte binding, as well as platelet P-selectin expression, compared to healthy subjects, whereas platelet expression of THPOR did not differ between the two groups. THPO concentrations correlated with platelet–leukocyte binding, as well as with fasting glucose and Hb1Ac. In vitro, plasma from diabetic patients, but not from healthy subjects, primed platelet–leukocyte binding and platelet P-selectin expression. Blocking THPO biological activity using a specific inhibitor prevented the priming effect induced by plasma from diabetic patients. In conclusion, augmented THPO may enhance platelet activation in patients with T1DM, potentially participating in increasing atherosclerotic risk.

scholarly journals Association of type 1 diabetes mellitus (DM1) with polymorphous alleles of class II HLA genes in Yakutian and Russian populations

2009 ◽  
Vol 12 (3) ◽  
pp. 26-32 ◽  
Author(s):  
Elena Vital'evna Titovich ◽  
Tamara Leonidovna Kuraeva ◽  
G I Danilova ◽  
L P Alekseev ◽  
M N Boldyreva ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Relative Risk ◽  
Type 1 Diabetes Mellitus ◽  
Healthy Subjects ◽  
Random Samples ◽  
Hla Genotyping ◽  
Successive Generations ◽  
Moscow Population

Materials and methods. HLA genotyping was accomplished in 51 DM1 patients and 51 volunteers randomly selected from the indigenous populationof Yakutia (Yakuts in three successive generations). Another 205 DM1 patients and 300 healthy subjects comprised random samples of patients andcontrols respectively from residents of Moscow and Moscow region. Results. HLA DRB1*17(03) allele proved to be the strongest one predisposing to DM1 in the Yakutian population (relative risk, RR=8,47) andDQB1*0304 in the Moscow population (RR=8,94). The presence of DRB1*04, DRB1*17(03), DQA1*0301, DQB1*0201, and DQB1*0302 accountedfor RR >2 in both populations. Only two alleles, DRB1*04 and DRB17(03), in the Yakutian population and five of the six (DRB1*04,DRB1*17(03), DQA1*(0301), DQB1*0302, and DQB1*0304) in the Moscow one were closely associated with DM1 (RR >4). DRB1*09, DRB1*11,DQB1*13, DQB1*0602/8 in Yakutian and DRB1*11, DRB1*13, DQA1*0103, DQB1*0301, DQB1*0602/8 in Moscow populations had the highestprotective potential (RR

scholarly journals Association of the polymorphous marker G6230A of the CTLA4 gene with type 1 diabetes mellitus in the patients of Russian descent

2012 ◽  
Vol 58 (4) ◽  
pp. 14-17
Author(s):  
O I Kopylova ◽  
T L Kuraeva ◽  
E Iu Lavrikova ◽  
E V Titovich ◽  
A G Nikitin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Healthy Subjects ◽  
Molecular Mechanisms ◽  
Control Group ◽  
Diabetic Patients ◽  
Potential Candidate ◽  
Ctla4 Gene

The risk of devolvement of type 1 diabetes mellitus (DM1) remains a challenging problem because neither etiology of the disease nor its prognosis and genetic predisposition to this condition are clearly understood. The development of any autoimmune process starts from the disturbance of subtle molecular mechanisms involved in the regulation of the immune system. Therefore, the genes controlling the function of its major components are at the same time the potential candidate genes encoding for the predisposition to DM1. Their association with the disease was studied by means of comparative analysis of the frequency distribution of alleles and genotypes of the polymorphous rs3087243 (G6230A) marker of the CTLA4 gene encoding for antigen-4 of cytotoxic T-lymphocytes. The present study included 257 patients presenting with type 1 diabetes mellitus and 526 healthy subjects. Genotypes were identified by the "real time" amplification technique. The AA genotype was found to occur less frequently in the diabetic patients than in the control group (11.3% and 22.1% respectively). In contrast, the frequency of the GG genotype was higher in the patients with DM1 than in the healthy subjects (44.7% and 37.5% respectively). It is concluded that the polymorphous rs3087243 marker of the CTLA4 gene is significantly associated with the predisposition to the development of type 1 diabetes mellitus in the patients of Russian descent.

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