scholarly journals Ultra-High Packing Density Next Generation Microtube Array Membrane for Absorption Based Applications

Membranes ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 273
Author(s):  
Chee Ho Chew ◽  
Wan-Ting Huang ◽  
Tzu-Sen Yang ◽  
Amanda Chen ◽  
Yun Ming Wu ◽  
...  
Keyword(s):  
Packing Density ◽  
Unit Area ◽  
Polymyxin B ◽  
The Novel ◽  
Next Generation ◽  
Rapid Reduction ◽  
Different Types ◽  
Set Up ◽  
Microtube Array ◽  
Time Point

Previously, we successfully developed an extracorporeal endotoxin removal device (EERD) that is based on the novel next generation alternating microtube array membrane (MTAM-A) that was superior to the commercial equivalent. In this article, we demonstrated multiple different parameter modifications that led to multiple different types of novel new MTAM structures, which ultimately led to the formation of the MTAM-A. Contrary to the single layered MTAM, the MTAM-A series consisted of a superior packing density fiber connected in a double layered, alternating position which allowed for the greater fiber count to be packed per unit area. The respective MTAM variants were electrospun by utilizing our internally developed tri-axial electrospinning set up to produce the novel microstructures as seen in the respective MTAM variants. A key uniqueness of this study is the ability to produce self-arranged fibers into the respective MTAM variants by utilizing a single spinneret, which has not been demonstrated before. Of the MTAM variants, we observed a change in the microstructure from a single layered MTAM to the MTAM-A series when the ratio of surfactant to shell flow rate approaches 1:1.92. MTAM-A registered the greatest surface area of 2.2 times compared to the traditional single layered MTAM, with the greatest tensile strength at 1.02 ± 0.13 MPa and a maximum elongation of 57.70 ± 9.42%. The MTAM-A was selected for downstream immobilization of polymyxin B (PMB) and assembly into our own internally developed and fabricated dialyzer housing. Subsequently, the entire setup was tested with whole blood spiked with endotoxin; and benchmarked against commercial Toraymyxin fibers of the same size. The results demonstrated that the EERD based on the MTAM-A performed superior to that of the commercial equivalent, registering a rapid reduction of 73.18% of endotoxin (vs. Toraymyxin at 38.78%) at time point 15 min and a final total endotoxin removal of 89.43% (vs. Toraymyxin at 65.03%)

Ultrahigh packing density next generation microtube array membrane: A novel solution for absorption‐based extracorporeal endotoxin removal device

2020 ◽  
Vol 108 (7) ◽  
pp. 2903-2911
Author(s):  
Chee Ho Chew ◽  
Li‐Wei Cheng ◽  
Wan‐Ting Huang ◽  
Yun Ming Wu ◽  
Chih‐Wei Lee ◽  
...  
Keyword(s):  
Packing Density ◽  
Next Generation ◽  
Microtube Array

On the Influence of the Slot Orifice in Diesel Common Rail Nozzle

2018 ◽  
Vol 11 (1) ◽  
pp. 55-69 ◽  
Author(s):  
Giancarlo Chiatti ◽  
Ornella Chiavola ◽  
Fulvio Palmieri ◽  
Roberto Pompei
Keyword(s):  
Cross Section ◽  
Flow Rate ◽  
Experimental Analysis ◽  
Common Rail ◽  
Spray Angle ◽  
The Novel ◽  
Hydraulic Behavior ◽  
Reference Information ◽  
Light Duty ◽  
Set Up

Background:The paper deals with a diesel common rail nozzle in which a novel orifice layout is implemented.Objective:Its influence on the nozzle mechanical-hydraulic behavior and on the spray shape transient development is experimentally investigated.Methods:In the research, a solenoid injector for light duty diesel engines is equipped with the novel nozzle prototype and tested. The prototype layout is described, pointing out the features of the nozzle orifices, in which a Slot cross-section is adopted; the investigation is accomplished extending the hydraulic tests and the spray visualizations to a reference nozzle with standard holes. The influence of the hole layout on the mechanical-hydraulic behavior of the nozzle is assessed by experimental analysis based on the rate of injection measurement, in comparison with the reference nozzle. Once the hydraulic behavior of the novel nozzle has been characterized in terms of mass flow rate, the slot influence on the spray shape is assessed analyzing the macroscopic features such as the penetration distance and the spray angle, in non evaporative conditions. The study is carried out under transient injection conditions, for different injection pressures, up to 1400 bar.Results:The results on spray characteristics also provide reference information to set up spray models suited to take the Slot orifice into account.

PERUBAHAN NILAI BUDAYA MINANGKABAU DALAM NOVEL TENGGELAMNYA KAPAL VAN DER WIJCK KARYA HAMKA DAN NOVEL BULAN SUSUT KARYA ISMET FANANY (KAJIAN PERBANDINGAN)

Jurnal KATA ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 336
Author(s):  
Yulia Pebriani
Keyword(s):  
Modern Society ◽  
Local Culture ◽  
The Novel ◽  
Next Generation ◽  
The Status ◽  
Symbolic Leadership ◽  
Cultural Shifts ◽  
Time Lead

<em>Local culture is very diverse Indonesia became an honor and challenge to maintain and inherited to the next generation. Local Indonesian culture is very proud because it has a very varied diversity and unique. As time, lead to changes in lifestyle a more modern society. As a result, people will prefer the new culture that may be considered more practical than the local culture. Views on kinship, treasures, and wander in the novel Tenggelamnya Kapal Van Der Wijck Hamka works and novels Bulan Susut works Ismet Fanany changes and cultural shifts. Kinship, treasures, and wander in the novel Sinking Ship Van Der Wijck Hamka's work is described explicitly, whereas kinship, treasures, and wander in the novel Month Losses Ismet work Fanany described implicitly. Changes in people's lives has implications for social Minangkabau culture in Minangkabau society. A leadership that is both functional mamak transformed into symbolic leadership. Mamak originally as straps tribesmen, has changed the status and intrinsic meaning.</em>

Characterization of the novel HLA‐C *07:944 allele by next‐generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Maria Loginova ◽  
Olga Makhova ◽  
Daria Smirnova ◽  
Igor Paramonov ◽  
Maksim Zarubin
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Generation Sequencing

Characterization of the novel HLA‐B *51:296 allele by next‐generation sequencing

HLA ◽  
2020 ◽  
Author(s):  
Steve Genebrier ◽  
Vincent Elsermans ◽  
Emeric Texeraud ◽  
Gerald Bertrand ◽  
Virginie Renac
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals CRISPR-Cas System: The Powerful Modulator of Accessory Genomes in Prokaryotes

2021 ◽  
pp. 1-16
Author(s):  
Anca Butiuc-Keul ◽  
Anca Farkas ◽  
Rahela Carpa ◽  
Dumitrana Iordache
Keyword(s):  
Nucleic Acids ◽  
Pathogenic Bacteria ◽  
The Novel ◽  
Defense System ◽  
Guide Rnas ◽  
Crispr Array ◽  
Different Types ◽  
Evolutionary Trajectories ◽  
Novel Coronavirus ◽  
Cas Proteins

Being frequently exposed to foreign nucleic acids, bacteria and archaea have developed an ingenious adaptive defense system, called CRISPR-Cas. The system is composed of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) array, together with CRISPR (<i>cas</i>)-associated genes. This system consists of a complex machinery that integrates fragments of foreign nucleic acids from viruses and mobile genetic elements (MGEs), into CRISPR arrays. The inserted segments (spacers) are transcribed and then used by cas proteins as guide RNAs for recognition and inactivation of the targets. Different types and families of CRISPR-Cas systems consist of distinct adaptation and effector modules with evolutionary trajectories, partially independent. The origin of the effector modules and the mechanism of spacer integration/deletion is far less clear. A review of the most recent data regarding the structure, ecology, and evolution of CRISPR-Cas systems and their role in the modulation of accessory genomes in prokaryotes is proposed in this article. The CRISPR-Cas system&apos;s impact on the physiology and ecology of prokaryotes, modulation of horizontal gene transfer events, is also discussed here. This system gained popularity after it was proposed as a tool for plant and animal embryo editing, in cancer therapy, as antimicrobial against pathogenic bacteria, and even for combating the novel coronavirus – SARS-CoV-2; thus, the newest and promising applications are reviewed as well.

Identification of the novel HLA‐DQB1 *05:275 allele by next‐generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Charlène Bouthemy ◽  
Mamy Ralazamahaleo ◽  
Jonathan Visentin ◽  
Marylise Fort ◽  
Nicolas Congy‐Jolivet
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Generation Sequencing

Characterization of the novel HLA‐DQA1 *05:19 allele by next‐generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Steve Genebrier ◽  
Paul Rouzaire ◽  
Emeric Texeraud ◽  
Gerald Bertrand ◽  
Virginie Renac
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Hla Dqa1 ◽  
Generation Sequencing

scholarly journals Evaluation of Ion Torrent next-generation sequencing for thalassemia diagnosis

2020 ◽  
Vol 48 (12) ◽  
pp. 030006052096777
Author(s):  
Peisong Chen ◽  
Xuegao Yu ◽  
Hao Huang ◽  
Wentao Zeng ◽  
Xiaohong He ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Ion Torrent ◽  
Next Generation ◽  
Large Deletions ◽  
Different Types ◽  
Variant Detection ◽  
Polymerase Chain ◽  
Next Generation Sequencing Ngs ◽  
Accuracy And Repeatability ◽  
Generation Sequencing

Introduction To evaluate a next-generation sequencing (NGS) workflow in the screening and diagnosis of thalassemia. Methods In this prospective study, blood samples were obtained from people undergoing genetic screening for thalassemia at our centre in Guangzhou, China. Genomic DNA was polymerase chain reaction (PCR)-amplified and sequenced using the Ion Torrent system and results compared with traditional genetic analyses. Results Of the 359 subjects, 148 (41%) were confirmed to have thalassemia. Variant detection identified 35 different types including the most common. Identification of the mutational sites by NGS were consistent with those identified by Sanger sequencing and Gap-PCR. The sensitivity and specificities of the Ion Torrent NGS were 100%. In a separate test of 16 samples, results were consistent when repeated ten times. Conclusion Our NGS workflow based on the Ion Torrent sequencer was successful in the detection of large deletions and non-deletional defects in thalassemia with high accuracy and repeatability.

Characterization of the novel HLA‐DQA1 *01:80 allele by next generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Valentine Jacob ◽  
Fabienne Farce ◽  
Rangolie Kaveri ◽  
Judith Desoutter ◽  
Nicolas Guillaume
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Hla Dqa1 ◽  
Generation Sequencing
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