scholarly journals Ultrasonic Particle Manipulation in Glass Capillaries: A Concise Review

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 876
Author(s):  
Guotian Liu ◽  
Junjun Lei ◽  
Feng Cheng ◽  
Kemin Li ◽  
Xuanrong Ji ◽  
...  
Keyword(s):  
Cross Sections ◽  
Ultrasonic Waves ◽  
Submicron Particles ◽  
Label Free ◽  
Particle Manipulation ◽  
Glass Capillary ◽  
Ultrasonic Particle Manipulation ◽  
Glass Capillaries ◽  
Acoustic Tweezers ◽  
Underlying Mechanisms

Ultrasonic particle manipulation (UPM), a non-contact and label-free method that uses ultrasonic waves to manipulate micro- or nano-scale particles, has recently gained significant attention in the microfluidics community. Moreover, glass is optically transparent and has dimensional stability, distinct acoustic impedance to water and a high acoustic quality factor, making it an excellent material for constructing chambers for ultrasonic resonators. Over the past several decades, glass capillaries are increasingly designed for a variety of UPMs, e.g., patterning, focusing, trapping and transporting of micron or submicron particles. Herein, we review established and emerging glass capillary-transducer devices, describing their underlying mechanisms of operation, with special emphasis on the application of glass capillaries with fluid channels of various cross-sections (i.e., rectangular, square and circular) on UPM. We believe that this review will provide a superior guidance for the design of glass capillary-based UPM devices for acoustic tweezers-based research.

Quantitative proteomics identifies FOLR1 to drive sorafenib resistance via activating autophagy in hepatocellular carcinoma cells

2021 ◽  
Author(s):  
Hongwei Chu ◽  
Changqing Wu ◽  
Qun Zhao ◽  
Rui Sun ◽  
Kuo Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Folate Receptor ◽  
Mass Spectrometry Analysis ◽  
Label Free ◽  
Spectrometry Analysis ◽  
Underlying Mechanisms ◽  
Related Proteins ◽  
Hcc Cells

Abstract Sorafenib is commonly used to treat advanced human hepatocellular carcinoma (HCC). However, clinical efficacy has been limited by drug resistance. In this study, we used label-free quantitative proteomic analysis to systematically investigate the underlying mechanisms of sorafenib resistance in HCC cells. A total of 1709 proteins were confidently quantified. Among them, 89 were differentially expressed, and highly enriched in the processes of cell-cell adhesion, negative regulation of apoptosis, response to drug and metabolic processes involving in sorafenib resistance. Notably, folate receptor α (FOLR1) was found to be significantly upregulated in resistant HCC cells. In addition, in-vitro studies showed that overexpression of FOLR1 decreased the sensitivity of HCC cells to sorafenib, whereas siRNA-directed knockdown of FOLR1 increased the sensitivity of HCC cells to sorafenib. Immunoprecipitation-mass spectrometry analysis suggested a strong link between FOLR1 and autophagy related proteins. Further biological experiments found that FOLR1-related sorafenib resistance was accompanied by the activation of autophagy, whereas inhibition of autophagy significantly reduced FOLR1-induced cell resistance. These results suggest the driving role of FOLR1 in HCC resistance to sorafenib, which may be exerted through FOLR1-induced autophagy. Therefore, this study may provide new insights into understanding the mechanism of sorafenib resistance.

scholarly journals Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 744 ◽  
Author(s):  
Xiangchun Xuan
Keyword(s):  
Continuous Flow ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Magnetic Fluids ◽  
Label Free ◽  
Particle Manipulation ◽  
The Past ◽  
Recent Advances ◽  
Medium Exchange ◽  
Flow Particle

Magnetic field-induced particle manipulation is simple and economic as compared to other techniques (e.g., electric, acoustic, and optical) for lab-on-a-chip applications. However, traditional magnetic controls require the particles to be manipulated being magnetizable, which renders it necessary to magnetically label particles that are almost exclusively diamagnetic in nature. In the past decade, magnetic fluids including paramagnetic solutions and ferrofluids have been increasingly used in microfluidic devices to implement label-free manipulations of various types of particles (both synthetic and biological). We review herein the recent advances in this field with focus upon the continuous-flow particle manipulations. Specifically, we review the reported studies on the negative magnetophoresis-induced deflection, focusing, enrichment, separation, and medium exchange of diamagnetic particles in the continuous flow of magnetic fluids through microchannels.

scholarly journals Wave number–spiral acoustic tweezers for dynamic and reconfigurable manipulation of particles and cells

2019 ◽  
Vol 5 (5) ◽  
pp. eaau6062 ◽  
Author(s):  
Zhenhua Tian ◽  
Shujie Yang ◽  
Po-Hsun Huang ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Cell Interaction ◽  
Cell Manipulation ◽  
Wave Number ◽  
Label Free ◽  
Multiple Functions ◽  
Pressure Distributions ◽  
Acoustic Tweezers ◽  
Interaction Research

Acoustic tweezers have recently raised great interest across many fields including biology, chemistry, engineering, and medicine, as they can perform contactless, label-free, biocompatible, and precise manipulation of particles and cells. Here, we present wave number–spiral acoustic tweezers, which are capable of dynamically reshaping surface acoustic wave (SAW) wavefields to various pressure distributions to facilitate dynamic and programmable particle/cell manipulation. SAWs propagating in multiple directions can be simultaneously and independently controlled by simply modulating the multitone excitation signals. This allows for dynamic reshaping of SAW wavefields to desired distributions, thus achieving programmable particle/cell manipulation. We experimentally demonstrated the multiple functions of wave number–spiral acoustic tweezers, among which are multiconfiguration patterning; parallel merging; pattern translation, transformation, and rotation; and dynamic translation of single microparticles along complex paths. This wave number–spiral design has the potential to revolutionize future acoustic tweezers development and advance many applications, including microscale assembly, bioprinting, and cell-cell interaction research.

scholarly journals Anodisation of Aluminium Alloys by Micro-Capillary Technique as a Tool for Reliable, Cost-Efficient, and Quick Process Parameter Determination

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Daniela Nickel ◽  
Dagmar Dietrich ◽  
Roy Morgenstern ◽  
Ingolf Scharf ◽  
Harry Podlesak ◽  
...  
Keyword(s):  
Process Parameters ◽  
Cross Sections ◽  
Aluminium Alloys ◽  
Focused Ion Beam ◽  
Corrosion Behaviour ◽  
Film Formation ◽  
Ion Beam ◽  
Parameter Determination ◽  
Pure Aluminium ◽  
Glass Capillary

Anodisation is essential for improving surface properties of aluminium alloys and composites regarding wear and corrosion behaviour. Optimisation of the anodising process depends on microstructural constituents contained in aluminium alloys and represents a key task, consisting of the control of process parameters and electrolyte formulation. We applied the micro-capillary technique known from corrosion studies and modified it to form anodic aluminium oxide films on high-strength aluminium alloys in comparison to pure aluminium in sulphuric acid. A glass capillary with an opening of 800 μm in diameter was utilized. Corresponding electrochemical measurements during potentiodynamic and potentiostatic anodisation revealed anodic current responses similar to conventional anodisation. The measurement of film thickness was adapted to the thin anodised spots using ellipsometry and energy dispersive X-ray analysis. Cross sections prepared by focused ion beam milling confirm the thickness results and show the behaviour of intermetallic phases depending on the anodising potential. Consequently, micro-capillary anodising proved to be an effective tool for developing appropriate anodisation conditions for aluminium alloys and composites because it allows quick variation of electrolyte composition by applying low electrolyte volumes and rapid film formation due to short process durations at small areas and more flexible variation of process parameters due to the used set-up.

Transducer arrays for ultrasonic particle manipulation

2010 ◽  
Author(s):  
Christine Demore ◽  
Yongqiang Qiu ◽  
Sandy Cochran ◽  
Peter Glynne-Jones ◽  
Congwei Ye ◽  
...  
Keyword(s):  
Particle Manipulation ◽  
Ultrasonic Particle Manipulation ◽  
Transducer Arrays

scholarly journals Proof of principle study of ultrasonic particle manipulation by a circular array device

2012 ◽  
Vol 468 (2147) ◽  
pp. 3571-3586 ◽  
Author(s):  
Alon Grinenko ◽  
Paul D. Wilcox ◽  
Charles R. P. Courtney ◽  
Bruce W. Drinkwater
Keyword(s):  
Finite Element ◽  
Finite Element Model ◽  
Field Distribution ◽  
Feasibility Study ◽  
Element Model ◽  
Circular Array ◽  
Particle Manipulation ◽  
Ultrasonic Particle Manipulation ◽  
Acoustic Array ◽  
Proof Of Principle

A feasibility study of a circular ultrasonic array device for acoustic particle manipulation is presented. A general approach based on Green's function is developed to analyse the underlying properties of a circular acoustic array. It allows the size of a controllable device area as a function of the number of array elements to be established and the array excitation required to produce a desired field distribution to be determined. A set of quantitative parameters characterizing the complexity of the pressure landscape is suggested, and relation to the number of array elements is found. Next, a finite-element model of a physically realizable circular piezo-acoustic array device is employed to demonstrate that the trapping capability can be achieved in practice.

scholarly journals Modelling for the robust design of layered resonators for ultrasonic particle manipulation

Ultrasonics ◽  
2008 ◽  
Vol 48 (6-7) ◽  
pp. 521-528 ◽  
Author(s):  
Martyn Hill ◽  
Rosemary J. Townsend ◽  
Nicholas R. Harris
Keyword(s):  
Robust Design ◽  
Particle Manipulation ◽  
Ultrasonic Particle Manipulation

Influence of acoustic streaming on ultrasonic particle manipulation in a 100-well ring-transducer microplate

2013 ◽  
Vol 23 (3) ◽  
pp. 035008 ◽  
Author(s):  
Mathias Ohlin ◽  
Athanasia E Christakou ◽  
Thomas Frisk ◽  
Björn Önfelt ◽  
Martin Wiklund
Keyword(s):  
Acoustic Streaming ◽  
Particle Manipulation ◽  
Ultrasonic Particle Manipulation

scholarly journals Micro-particle manipulation by single beam acoustic tweezers based on hydrothermal PZT thick film

AIP Advances ◽  
2016 ◽  
Vol 6 (3) ◽  
pp. 035102 ◽  
Author(s):  
Benpeng Zhu ◽  
Jiong Xu ◽  
Ying Li ◽  
Tian Wang ◽  
Ke Xiong ◽  
...  
Keyword(s):  
Thick Film ◽  
Particle Manipulation ◽  
Single Beam ◽  
Acoustic Tweezers ◽  
Pzt Thick Film

scholarly journals Ponatinib induces vascular toxicity through the Notch-1 signaling pathway

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
R Madonna ◽  
D Pieragostino ◽  
M Cufaro ◽  
V Doria ◽  
P Del Boccio ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Vascular Dysfunction ◽  
Tube Formation ◽  
Label Free ◽  
Notch 1 ◽  
Vascular Toxicity ◽  
Selective Blockade ◽  
Phosphorylated Akt ◽  
Underlying Mechanisms

Abstract Background Ponatinib, a third-generation tyrosine kinase inhibitor (TKI), is the only approved TKI that is effective against T315I mutations in patients with chronic myeloid leukemia (CML). Specific activation of Notch signaling in CML cells by ponatinib can be considered as the “on-target effect” on the tumor and represents a therapeutic approach for CML. Nevertheless, ponatinib-induced vascular toxicity remains a serious concern, with underlying mechanisms poorly understood. Aims We aimed at determining mechanisms of ponatinib-induced vascular toxicity, defining associated signaling pathways and identifying potential rescue strategies. Methods and results We exposed human umbilical endothelial cells (HUVECs) to ponatinib or vehicle in the presence or absence of the neutralizing factor anti-Notch-1 antibody for exposure times of 0–72 hours. Label-free proteomics and network analysis showed that protein cargo of HUVECs treated with ponatinib triggered apoptosis, and inhibited vasculature development (Fig. 1). We validated the proteomic data showing the inhibition of matrigel tube formation, an upregulation of cleaved caspase-3 and a downregulation of phosphorylated AKT and phosphorylated eNOS. We delineated the signaling of ponatinib-induced vascular toxicity demonstrating that ponatinib inhibits endothelial survival, reduces angiogenesis and induces endothelial senescence and apoptosis via Notch-1 pathway. Conclusion Ponatinib induced endothelial toxicity in vitro. Hyperactivation of Notch-1 in the vessels can lead to abnormal vascular development and vascular dysfunction. By hyperactivating Notch-1 in the vessels, ponatinib exerts an “on-target off turmor effect”, which leads to deleterious effects and may explain the drug's vasculotoxicity. Selective blockade of Notch-1 prevented ponatinib-induced vascular toxicity. Figure 1 Funding Acknowledgement Type of funding source: None

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