scholarly journals High-Throughput Sequencing Analysis of the Composition and Diversity of the Bacterial Community in Cinnamomum camphora Soil

2021 ◽  
Vol 10 (1) ◽  
pp. 72
Author(s):  
Deqiang Chen ◽  
Weihong Sun ◽  
Shuang Xiang ◽  
Shuangquan Zou
Keyword(s):  
High Throughput ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Bulk Soil ◽  
Slope Position ◽  
Available Phosphorus ◽  
Sequencing Analysis ◽  
Sample Type ◽  
Cinnamomum Camphora ◽  
And Function

Soil bacterial communities and root-associated microbiomes play important roles in the nutrient absorption and healthy growth of host plants. Cinnamomum camphora is an important timber and special economic forest tree species in Fujian Province. In this study, the high-throughput sequencing technique was used to analyze the composition, diversity, and function of the bacterial communities present in the soil from different samples and slope positions of C. camphora. The results of this analysis demonstrated that the related bacterial communities in C. camphora soil were mainly clustered based on sample type. Bacterial alpha diversity in the rhizosphere and bulk soil of C. camphora growing downhill was higher than that of C. camphora growing uphill. At the phylum level, Bacteroidetes, Proteobacteria, Chloroflexi, and Gemmatimonadetes were positively correlated with pH, available phosphorus, total phosphorus, available potassium, and total potassium, while Acidobacteria and Verrucomicrobia were negatively correlated with alkaline-hydrolyzable nitrogen. These results show that there were remarkable differences in the composition, diversity, and function of related bacterial communities between different sample types of C. camphora soil. The slope position had a marked effect on the bacterial communities in the rhizosphere and bulk soil, while the root endosphere remained unaffected.

Defunct gold mine tailings are natural reservoir for unique bacterial communities revealed by high-throughput sequencing analysis

2019 ◽  
Vol 650 ◽  
pp. 2199-2209 ◽  
Author(s):  
Timothy Sibanda ◽  
Ramganesh Selvarajan ◽  
Titus Msagati ◽  
Siddarthan Venkatachalam ◽  
Stephen Meddows-Taylor
Keyword(s):  
High Throughput ◽  
Mine Tailings ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Gold Mine ◽  
Sequencing Analysis ◽  
Natural Reservoir ◽  
Gold Mine Tailings

scholarly journals High-throughput Sequencing Analysis of the Rhizosphere Arbuscular Mycorrhizal Fungi (AMF) Community Composition Associated with Ferula Sinkiangensis

2020 ◽  
Author(s):  
Yunfeng Luo ◽  
Yaling He ◽  
Guifang Li ◽  
Xinhua Lv ◽  
Zhongke Wang ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Community Composition ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Abiotic Factors ◽  
Slope Position ◽  
Sequencing Analysis ◽  
Soil Physicochemical Properties ◽  
Ferula Sinkiangensis ◽  
The Relationship

Abstract Background: Ferula sinkiangensis is an important and increasingly endangered medicinal plant. Arbuscular mycorrhiza fungi (AMF) are microbes that live in the soil and can enhance nutrient uptake, stress resistance, and pathogen defens in host plants. The composition of AMF communities associated with Ferula sinkiangensis and the relationship between these fungi and other pertinent abiotic factors, however, remains uncertain. Herein, we collected samples of rhizosphere and surrounding soil at a range of depths (0-20, 20-40, and 40-60 cm) and a range of slop positions (bottom, middle, top). These samples were then subjected to analyses of soil physicochemical properties and high-throughput sequencing (Illumina MiSeq), enabling us to examine AMF community composition and diversity, as well as the relationship between these parameters and other abiotic factors. Results: Through this analysis, we determined that Glomus and Diversispora were enriched in all samples. AMF diversity and richness varied significantly as a function of slope position, with this variation primarily being tied to differences in Glomus and Diversispora abundance. In contrast, no significant relationship between soil depth and overall AMF composition was noted, although some AMF were found to be sensitive to depth. Many factors were found to significantly affect AMF community composition, including organic matter (OM), TN (total nitrogen), TK (total potassium), AN (ammonium nitrogen), NN (nitrate nitrogen), AK (available potassium), TDS (total soluble salt), pH, SM (soil water content), and AE (slope position). We further found that Shannon diversity index values in these communities were positively correlated with TP (total phosphorus), NN, and pH values (p<0.05), while TP, TDS, and pH were positively correlated with Chao1 values (p<0.05). Conclusion: In summary, these findings reveal that Glomus and Diversispora are key AMF genera found within the rhizosphere soil of Ferula sinkiangensis. These fungi are closely associated with specific environmental and soil physicochemical properties. And The physical and chemical properties of soil were significantly different (p<0.05) because of slope position. Together, our results provide a novel understanding of the relationship between AMF species and Ferula sinkiangensis, providing a theoretical basis for further studies of their development.

High‐throughput sequencing‐based analysis of bacterial communities associated with Barbour’s seahorses ( Hippocampus barbouri ) from Surigao del Norte, Philippines

2021 ◽  
Author(s):  
Rose Chinly Mae H. Ortega ◽  
Sharon Rose M. Tabugo ◽  
Joey Genevieve T. Martinez ◽  
Chinee S. Padasas ◽  
Marilen P. Balolong ◽  
...  
Keyword(s):  
High Throughput ◽  
Bacterial Communities ◽  
High Throughput Sequencing
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