scholarly journals High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222191 ◽  
Author(s):  
Meidan He ◽  
Jiachao Zhang ◽  
Linbo Shen ◽  
Lixin Xu ◽  
Wenjie Luo ◽  
...  

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168166 ◽  
Author(s):  
Lin Yang ◽  
Hui-lin Yang ◽  
Zong-cai Tu ◽  
Xiao-lan Wang


PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193097 ◽  
Author(s):  
Yu-jie Wei ◽  
Yun Wu ◽  
Yin-zhuo Yan ◽  
Wan Zou ◽  
Jie Xue ◽  
...  


2015 ◽  
Vol 65 (3) ◽  
pp. 1741-1751 ◽  
Author(s):  
Wenjun Liu ◽  
Xiaoxia Xi ◽  
Qimuge Sudu ◽  
Laiyu Kwok ◽  
Zhuang Guo ◽  
...  


2018 ◽  
Vol 20 (4) ◽  
pp. 673-685 ◽  
Author(s):  
Tatiana A. Vishnivetskaya ◽  
Haiyan Hu ◽  
Joy D. Van Nostrand ◽  
Ann M. Wymore ◽  
Xiaohang Xu ◽  
...  

Sulfate-reducing bacteria and methanogens are the primary Hg-methylators in Chinese rice paddies.



2020 ◽  
Author(s):  
Yan Xu ◽  
Junfeng Niu ◽  
Lijun Chen ◽  
Xiaoqiang Wu ◽  
Zhongmin Dong ◽  
...  

Abstract Background Atractylodes lancea is a traditional Chinese medicine, which typically requires more than 3–4 years of continuous cropping to obtain the underground medicinal components. With continuous cropping years, the quality and yields of A. lancea medicinal materials decrease, while pests and diseases increase. These aspects are intimately correlated with rhizospheric microorganisms. Methods This research paper employed high-throughput sequencing for its detection in soil that was cultivated for three years and never cultivated to clarify the relationship between the microbial diversity of the rhizosphere and continuous A. lancea cropping. Results The rhizosphere microbial community was altered following the continuous cropping of A. lancea. The bacterial diversity and richness were observed to decrease, while the fungal community diversity increased, and richness decreased. The total OUTs of the soil bacteria and fungi of unplanted and planted A. lancea were 59.58% and 37.65%, respectively. At the phylum level, the relative abundance of Proteobacteria, Gemmatimonadetes, Acidobacteria and Chloroflexi decreased, whereas the relative abundance of Mortierellomycota increased. At the genus level, Bradyrhizobium, Striaticonidium, Dactylonectria, Sphingomonas, Burkholderiaceae, Rhodanobacter, Arthrobacter, Scleroderma, Mortierella and Penicillium were significantly different between the two sample groups. Conclusions Our results revealed that following the cultivation of A. lancea, the rhizospheric microbial community was altered. This study preliminarily determined the



2018 ◽  
Vol 83 ◽  
pp. 93-102 ◽  
Author(s):  
Jian-Hua Wang ◽  
Jian Lu ◽  
Yu-Xuan Zhang ◽  
Jun Wu ◽  
Cui Zhang ◽  
...  


2011 ◽  
Vol 7 (1) ◽  
pp. e1001061 ◽  
Author(s):  
Thomas J. Sharpton ◽  
Samantha J. Riesenfeld ◽  
Steven W. Kembel ◽  
Joshua Ladau ◽  
James P. O'Dwyer ◽  
...  


2021 ◽  
Vol 8 ◽  
Author(s):  
Haigang Wu ◽  
Xian Wu ◽  
Li Huang ◽  
Chongmei Ruan ◽  
Jinni Liu ◽  
...  

The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb Andrographis paniculata. Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the Firmicutes while Bacteroidetes dominated the amoxicillin treatment group compared with the negative controls. Specifically, the f__Lachnospiraceae_ Unclassified, Lachnospiraceae_ NK4A136_group and Ruminococcaceae_ UCG-014 were enriched with andrographolide administration while Bacteroides, Klebsiella and Escherichia-Shigella significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.



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