scholarly journals Stimulation of Nicotiana tabacum L. In Vitro Shoot Growth by Endophytic Bacillus cereus Group Bacteria

2021 ◽  
Vol 9 (9) ◽  
pp. 1893
Author(s):  
Elena Andriūnaitė ◽  
Inga Tamošiūnė ◽  
Monika Aleksandravičiūtė ◽  
Dalia Gelvonauskienė ◽  
Jurgita Vinskienė ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Endophytic Bacteria ◽  
Shoot Growth ◽  
Tissue Cultures ◽  
Bacillus Cereus Group ◽  
Nicotiana Tabacum L ◽  
Bacillus Spp ◽  
Plant Tissue Cultures ◽  
In Vitro Plant

In vitro plant tissue cultures face various unfavorable conditions, such as mechanical damage, osmotic shock, and phytohormone imbalance, which can be detrimental to culture viability, growth efficiency, and genetic stability. Recent studies have revealed a presence of diverse endophytic bacteria, suggesting that engineering of the endophytic microbiome of in vitro plant tissues has the potential to improve their acclimatization and growth. Therefore, the aim of this study was to identify cultivated tobacco (Nicotiana tabacum L.) endophytic bacteria isolates that are capable of promoting the biomass accumulation of in vitro tobacco shoots. Forty-five endophytic bacteria isolates were obtained from greenhouse-grown tobacco plant leaves and were assigned to seven Bacillus spp. and one Pseudomonas sp. based on 16S rRNA or genome sequence data. To evaluate the bacterial effect on in vitro plant growth, tobacco shoots were inoculated with 22 isolates selected from distinct taxonomic groups. Four isolates of Bacillus cereus group species B. toyonensis, B. wiedmannii and B. mycoides promoted shoot growth by 11–21%. Furthermore, a contrasting effect on shoot growth was found among several isolates of the same species, suggesting the presence of strain-specific interaction with the plant host. Comparative analysis of genome assemblies was performed on the two closely related B. toyonensis isolates with contrasting plant growth-modulating properties. This revealed distinct structures of the genomic regions, including a putative enzyme cluster involved in the biosynthesis of linear azol(in)e-containing peptides and polysaccharides. However, the function of these clusters and their significance in plant-promoting activity remains elusive, and the observed contrasting effects on shoot growth are more likely to result from genomic sequence variations leading to differences in metabolic or gene expression activity. The Bacillus spp. isolates with shoot-growth-promoting properties have a potential application in improving the growth of plant tissue cultures in vitro.

scholarly journals Growth and Division of Single Cells of Higher Plants in Vitro

1960 ◽  
Vol 43 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Ludwig Bergmann
Keyword(s):  
Nicotiana Tabacum ◽  
Higher Plants ◽  
Single Cells ◽  
Tissue Cultures ◽  
Phaseolus Vulgaris L ◽  
Isolated Cells ◽  
Nicotiana Tabacum L ◽  
Agar Layer ◽  
Petri Dishes

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

2007 ◽  
Vol 46 (11) ◽  
pp. 2138 ◽  
Author(s):  
Ana Celia Muñoz-Muñoz ◽  
Humberto Gutiérrez-Pulido ◽  
José Manuel Rodríguez-Domínguez ◽  
Antonia Gutiérrez-Mora ◽  
Benjamín Rodríguez-Garay ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Fluorescence Spectra ◽  
Laser Induced Fluorescence ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
In Vitro Plant

scholarly journals Antifungal Prenylated Diphenyl Ethers from Arthrinium arundinis, an Endophytic Fungus Isolated from the Leaves of Tobacco (Nicotiana tabacum L.)

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3179 ◽  
Author(s):  
Peng Zhang ◽  
Xin Li ◽  
Xiao-Long Yuan ◽  
Yong-Mei Du ◽  
Bin-Gui Wang ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Line ◽  
Endophytic Fungus ◽  
In Vitro Cytotoxicity ◽  
Ionization Mass ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Nicotiana Tabacum L ◽  
Diphenyl Ethers

An endophytic fungus Arthrinium arundinis TE-3 was isolated and purified from the fresh leaves of cultivated tobacco (Nicotiana tabacum L.). Chemical investigation on this fungal strain afforded three new prenylated diphenyl ethers (1−3) as well as three known analogues (4−6). Structure elucidation of the isolated compounds was carried out by analysis of 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS) spectra, as well as by comparison of those data with literature data. The absolute configuration of the stereogenic center at C-8 in 1 was assigned by comparison of the experimental and calculated ECD spectra. Compounds 1 and 2 showed selective antifungal activity against Mucor hiemalis with minimum inhibitory concentration (MIC) values of 8 and 4 μg/mL, respectively. Compounds 5 and 6 exhibited inhibitory activity against Alteraria alternata with an MIC value of 8 μg/mL. In the cytotoxic assay, 2, 5, and 6 displayed moderate in vitro cytotoxicity against the human monocytic cell line (THP-1 cell line), with IC50 values of 40.2, 28.3, and 25.9 μM, respectively. This study indicated that endophytic fungi possess great potential for exploring new bioactive secondary metabolites.

scholarly journals Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)

2021 ◽  
pp. 39-43
Author(s):  
Ákos Mendel ◽  
László Kovács ◽  
Erzsébet Kiss
Keyword(s):  
Nicotiana Tabacum ◽  
Salt Tolerance ◽  
Lower Class ◽  
Arginine Decarboxylase ◽  
Polyamine Metabolism ◽  
Spermine Synthase ◽  
Nicotiana Tabacum L ◽  
Exogenous Addition ◽  
Precursor Molecules

Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10 mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines.

Cytokinin autotrophy and differentiation in tissue cultures of haploid Nicotiana tabacum L.

Plant Science ◽  
1986 ◽  
Vol 45 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Gilberto Barbante Kerbauy ◽  
JoséAntonio Peters ◽  
Kurt Gunther Hell
Keyword(s):  
Nicotiana Tabacum ◽  
Tissue Cultures ◽  
Nicotiana Tabacum L

Correlation of PCB Transformation by Plant Tissue Cultures with Their Morphology and Peroxidase Activity Changes

1999 ◽  
Vol 64 (9) ◽  
pp. 1497-1509 ◽  
Author(s):  
Petra Kučerová ◽  
Martina Macková ◽  
Ludmila Poláchová ◽  
Jiří Burkhard ◽  
Kateřina Demnerová ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Polychlorinated Biphenyls ◽  
Peroxidase Activity ◽  
Plant Tissue ◽  
Plant Cells ◽  
Tissue Cultures ◽  
Transforming Activity ◽  
Plant Tissue Cultures ◽  
Transformation Capacity

The ability of plant cells cultivated in vitro to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism.

scholarly journals In vitro Degradation of 2,4,6-Trinitrotoluene Using Plant Tissue Cultures ofSolanum aviculare andRheum palmatum

2004 ◽  
Vol 4 (1) ◽  
pp. 46-49 ◽  
Author(s):  
A. Nepovím ◽  
M. Hubálek ◽  
R. Podlipná ◽  
S. Zeman ◽  
T. Vanek
Keyword(s):  
Plant Tissue ◽  
Tissue Cultures ◽  
In Vitro Degradation ◽  
Plant Tissue Cultures
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