scholarly journals Anthocyanin Encapsulated by Ferulic Acid-Grafted-Maltodextrin (FA-g-MD) Microcapsules Potentially Improved its Free Radical Scavenging Capabilities Against H2O2-Induced Oxidative Stress

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1596 ◽  
Author(s):  
Yi Ma ◽  
Yunhui Feng ◽  
Wanling Zeng ◽  
Huibo Luo
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Human Colon ◽  
Quinone Oxidoreductase ◽  
Human Colon Cancer ◽  
Glutamate Cysteine Ligase ◽  
Human Colon Cancer Cells ◽  
Biphasic Release ◽  
Ht 29

This study aimed to investigate the antioxidant activity and release behavior of anthocyanin (ANC) loaded within FA-g-MD wall (ANC-FA-g-MD microcapsule) in vitro. The microencapsulation of ANC was prepared by spray drying and displayed a biphasic release profile. The combination of ANC and FA-g-MD (0.0625–1 mg/mL) showed a higher antioxidant activity than that of both individuals. A possible intermolecular interaction between ANC and FA-g-MD was studied by UV-vis spectra. Intracellular reactive oxygen species (ROS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test, and protein expression of quinone oxidoreductase 1(NQO1), glutathione reductase (GSR) and γ-glutamate cysteine ligase catalytic subunit (γ-GCLC) were measured through human colon cancer cells (HT-29). After a 24-hour incubation of the HT-29, the combinations (0–60 μg/mL) exhibited a high potential to diminish the ROS level. And the distinct upregulated expressions of GCLC and NQO1 of HT-29 were detected after treatment with combinations compared to those of single ones. These results suggested that the ANC-FA-g-MD microcapsules exerts enhanced antioxidant effect with capability of the modulation of GCLC and NQO1.

scholarly journals EFFECT OF ANTITUMOR DRUGS IN LOW CONCENTRATIONS ON THE BIOLOGICAL, IMMUNOPHENOTYPIC AND CYTOGENETIC CHARACTERISTICS OF HUMAN COLON CANCER CELLS in vitro

2017 ◽  
Vol 39 (1) ◽  
pp. 17-24
Author(s):  
N Bezdieniezhnykh ◽  
O Kovalova ◽  
O Lykhova ◽  
R Kocherga ◽  
A Vorontsova ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Low Dose ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Cells With Micronuclei ◽  
Human Colon Cancer Cells ◽  
Ht 29

Objective: To estimate the impact of the low-dose anticancer drugs (ACD) with the different mechanisms of action and human interferon (IFN) alpha 2b on the biological properties, immunophenotypic and cytogenetic characteristics of colon cancer cells in vitro. Materials and Methods: The study was performed on human colon cancer cell lines COLO 205, HT-29 and 3C-P treated with ACD and IFN in subtoxic concentrations. Expression of CD44, N-cadherin, vimentin, β-catenin, ERCC1 and Slug was assessed by immunocytochemical method. Using cytogenetic analysis, the numbers of mitoses, cells with micronuclei, apoptotic cells and cells with nuclear protrusions were studied. Results: The prolonged exposure (up to 30 days) of colon cancer cells to low-dose ACD (0.2–0.5 µg/ml cisplatin and 0.1–0.2 µg/ml irinotecan) in combination with IFN (500–1000 IU/ml) led to 37-fold decreased colony-forming activity of these cell and 10-fold reduction of the number of cells expressing mesenchymal protein markers (N-cadherin, vimentin). Also, in COLO 205 cells treated with ACD and IFN the number of SLUG- and CD44-positive cells decreased by 92 and by 85%, respectively. Long-term cultivation of HT-29 cells in the presence of cisplatin and IFN resulted in 5-fold suppression of ERCC1 expression. The cytogenetic analysis has shown that the ACD, IFN and their combinations in subtoxic concentrations caused significant genotoxic effect, suppression of cell proliferation and accumulation of cells with micronuclei. The sensitivity of colon cancer cells to ACD in standard cytotoxic concentrations did not change after prolonged low-dose exposure. Conclusion: The data showed that the prolonged action of the low doses of ACD on human colon cancer cells resulted in the suppression of cell proliferation, colony-forming activity in soft agar, expression of epithelialmesenchymal transition-associated markers and significant cytogenetic changes.

scholarly journals P-0193 Transgelin Promotes The Invasiveness of Human Colon Cancer Cells in Vitro

2012 ◽  
Vol 23 ◽  
pp. iv85-iv86
Author(s):  
Ying Lin ◽  
Yuan-yuan Fang ◽  
Hong Su ◽  
Zhou Hui-Min ◽  
Qi-Kui Chen
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells
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