scholarly journals Discovery of a Nitric Oxide-Responsive Protein in Arabidopsis thaliana

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2691 ◽  
Author(s):  
Randa Zarban ◽  
Malvina Vogler ◽  
Aloysius Wong ◽  
Joerg Eppinger ◽  
Salim Al-Babili ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Arabidopsis Thaliana ◽  
Binding Proteins ◽  
Light Response ◽  
Heme Iron ◽  
Search Term ◽  
Interacting Protein ◽  
Broad Complex ◽  
Heme Cofactor ◽  
Selection Of

In plants, much like in animals, nitric oxide (NO) has been established as an important gaseous signaling molecule. However, contrary to animal systems, NO-sensitive or NO-responsive proteins that bind NO in the form of a sensor or participating in redox reactions have remained elusive. Here, we applied a search term constructed based on conserved and functionally annotated amino acids at the centers of Heme Nitric Oxide/Oxygen (H-NOX) domains in annotated and experimentally-tested gas-binding proteins from lower and higher eukaryotes, in order to identify candidate NO-binding proteins in Arabidopsis thaliana. The selection of candidate NO-binding proteins identified from the motif search was supported by structural modeling. This approach identified AtLRB3 (At4g01160), a member of the Light Response Bric-a-Brac/Tramtrack/Broad Complex (BTB) family, as a candidate NO-binding protein. AtLRB3 was heterologously expressed and purified, and then tested for NO-response. Spectroscopic data confirmed that AtLRB3 contains a histidine-ligated heme cofactor and importantly, the addition of NO to AtLRB3 yielded absorption characteristics reminiscent of canonical H-NOX proteins. Furthermore, substitution of the heme iron-coordinating histidine at the H-NOX center with a leucine strongly impaired the NO-response. Our finding therefore established AtLRB3 as a NO-interacting protein and future characterizations will focus on resolving the nature of this response.

scholarly journals The ATXN2 Orthologs CID3 and CID4, Act Redundantly to In-Fluence Developmental Pathways throughout the Life Cycle of Arabidopsis thaliana

2021 ◽  
Vol 22 (6) ◽  
pp. 3068
Author(s):  
Zaira M. López-Juárez ◽  
Laura Aguilar-Henonin ◽  
Plinio Guzmán
Keyword(s):  
Arabidopsis Thaliana ◽  
Life Cycle ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Leaf Growth ◽  
Transcriptome Profiling ◽  
Developmental Pathways ◽  
Model Organisms ◽  
Key Factors

RNA-binding proteins (RBPs) are key elements involved in post-transcriptional regulation. Ataxin-2 (ATXN2) is an evolutionarily conserved RBP protein, whose function has been studied in several model organisms, from Saccharomyces cerevisiae to the Homo sapiens. ATXN2 interacts with poly(A) binding proteins (PABP) and binds to specific sequences at the 3′UTR of target mRNAs to stabilize them. CTC-Interacting Domain3 (CID3) and CID4 are two ATXN2 orthologs present in plant genomes whose function is unknown. In the present study, phenotypical and transcriptome profiling were used to examine the role of CID3 and CID4 in Arabidopsis thaliana. We found that they act redundantly to influence pathways throughout the life cycle. cid3cid4 double mutant showed a delay in flowering time and a reduced rosette size. Transcriptome profiling revealed that key factors that promote floral transition and floral meristem identity were downregulated in cid3cid4 whereas the flowering repressor FLOWERING LOCUS C (FLC) was upregulated. Expression of key factors in the photoperiodic regulation of flowering and circadian clock pathways, were also altered in cid3cid4, as well as the expression of several transcription factors and miRNAs encoding genes involved in leaf growth dynamics. These findings reveal that ATXN2 orthologs may have a role in developmental pathways throughout the life cycle of plants.

Tissue culture of Arabidopsis thaliana explants reveals a stimulatory effect of alkamides on adventitious root formation and nitric oxide accumulation

Plant Science ◽  
2008 ◽  
Vol 174 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Juan Carlos Campos-Cuevas ◽  
Ramón Pelagio-Flores ◽  
Javier Raya-González ◽  
Alfonso Méndez-Bravo ◽  
Randy Ortiz-Castro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tissue Culture ◽  
Arabidopsis Thaliana ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation

Specific interactions between Dicer-like proteins and HYL1/DRB- family dsRNA-binding proteins in Arabidopsis thaliana

2005 ◽  
Vol 57 (2) ◽  
pp. 173-188 ◽  
Author(s):  
Akihiro Hiraguri ◽  
Riku Itoh ◽  
Naoko Kondo ◽  
Yasuko Nomura ◽  
Daisuke Aizawa ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Binding Proteins ◽  
Specific Interactions ◽  
Dsrna Binding

scholarly journals Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

2016 ◽  
Vol 7 ◽  
Author(s):  
Adil Hussain ◽  
Bong-Gyu Mun ◽  
Qari M. Imran ◽  
Sang-Uk Lee ◽  
Teferi A. Adamu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Arabidopsis Thaliana ◽  
Transcriptome Profiling ◽  
Regulatory Pathways

scholarly journals First person – Julien Pernier

2020 ◽  
Vol 133 (18) ◽  
pp. jcs253930
Keyword(s):  
Actin Filaments ◽  
Binding Proteins ◽  
Network Dynamics ◽  
Early Career ◽  
Actin Binding ◽  
First Person ◽  
Integrative Biology ◽  
Actin Network ◽  
Early Career Researchers ◽  
Selection Of

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Julien Pernier is first author on ‘Myosin 1b flattens and prunes branched actin filaments’, published in JCS. Julien conducted the research described in this article while a postdoc in Patricia Bassereau's lab at the Institut Curie, Paris, France. He is now a postdoc in the lab of Christophe Le Clainche at the Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France, investigating the roles of actin-binding proteins in actin network dynamics and organization.

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