scholarly journals Flavanone-Based Fluorophores with Aggregation-Induced Emission Enhancement Characteristics for Mitochondria-Imaging and Zebrafish-Imaging

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3298
Author(s):  
Na Li ◽  
Liyan Liu ◽  
Huiqing Luo ◽  
Huaqiao Wang ◽  
Depo Yang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Fluorescent Dyes ◽  
Fluorescence Emission ◽  
In Vivo Studies ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
High Fluorescence ◽  
Potential Use

Fluorophores with aggregation-induced emission enhancement (AIEE) characteristics applied in bioimaging have attracted more and more attention in recent years. In this work, a series of flavanone compounds with AIEE characteristics was developed and applied to fluorescence imaging of mitochondria and zebrafish. The compounds were readily prepared by the thermal dehydration of chalcone that was obtained by the reaction of o-hydroxyacetophenone and benzaldehyde. Two of these compounds showed significant AIEE characteristics by fluorescence performance experiments, including optical spectra, fluorescence spectra, fluorescence quantum yield (φF), fluorescence lifetime, and scanning electron microscopy (SEM). Compared with traditional organic fluorescent dyes, these compounds have high fluorescence emission and high fluorescence quantum yield in solid or aggregated state, which overcomes the shortcoming of aggregation-caused quenching (ACQ). More importantly, the two compounds exhibited low cytotoxicity and good cytocompatibility in A549 lung cells at the experimental concentration range and they specifically targeted mitochondria, which make it of great potential use in mitochondria labeling. In addition, they were embryonic membrane permeable and had different affinities for different tissues and organs of zebrafish, but mainly distributed in the digestive system, providing a basis for the application of such compounds in bioimaging. These AIEE compounds with superior properties could be of great potential use in mitochondria imaging and other in vivo studies.

scholarly journals Achieving remarkable and reversible mechanochromism from a bright ionic AIEgen with high specificity for mitochondrial imaging and secondary aggregation emission enhancement for long-term tracking of tumors

2020 ◽  
Vol 4 (3) ◽  
pp. 941-949 ◽  
Author(s):  
Xinzhe Yang ◽  
Qian Wang ◽  
Peiyu Hu ◽  
Chao Xu ◽  
Wenjing Guo ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescence Quantum Yield ◽  
High Specificity ◽  
Emission Enhancement ◽  
High Fluorescence ◽  
High Fluorescence Quantum Yield

An ionic AIEgen with high fluorescence quantum yield shows remarkable and reversible mechanochromism as well as excellent mitochondrial imaging of cancer cells and long-term tracking of tumors.

scholarly journals Synthesis, enantioseparation and photophysical properties of planar-chiral pillar[5]arene derivatives bearing fluorophore fragments

2019 ◽  
Vol 15 ◽  
pp. 1601-1611 ◽  
Author(s):  
Guojuan Li ◽  
Chunying Fan ◽  
Guo Cheng ◽  
Wanhua Wu ◽  
Cheng Yang
Keyword(s):  
Quantum Yield ◽  
Click Reaction ◽  
Photophysical Properties ◽  
High Yield ◽  
Mixed Solution ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
Emission Quenching ◽  
High Fluorescence ◽  
Induced Aggregation

Planar chiral pillar[5]arene derivatives (P5A-DPA and P5A-Py) bearing bulky fluorophores were obtained in high yield by click reaction. The photophysical properties of both compounds were investigated in detail. P5A-DPA with two 9,10-diphenylanthracene (DPA) pigments grafted on the pillar[5]arene showed a high fluorescence quantum yield of 89.5%. This is comparable to the monomer DPA-6, while P5A-Py with two perylene (Py) pigments grafted on the pillar[5]arene showed a significantly reduced quantum yield of 46.4% vs 78.2% for the monomer Py-6. The oxygen-through-annulus rotation of the phenolic units was inhibited for both compounds due to the bulky chromophore introduced, and the resolution of the enantiomers was achieved due to the bulky size of the fluorophores. The absolute configuration of the enantiomers was determined by circular dichroism (CD) spectra. The solvent-induced aggregation behavior was investigated with the enantiopure P5A-DPA and P5A-Py. It was found that the CD signals were enhanced by aggregation. P5A-DPA showed aggregation-induced emission enhancement, while P5A-Py showed aggregation-induced emission quenching, accompanied by excimer emission when aggregating in water and THF mixed solution.

scholarly journals Development of a high quantum yield dye for tumour imaging

2017 ◽  
Vol 8 (9) ◽  
pp. 6322-6326 ◽  
Author(s):  
Dan Yang ◽  
Huasen Wang ◽  
Chengjie Sun ◽  
Hui Zhao ◽  
Kuan Hu ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Fluorescent Dye ◽  
Tumour Imaging ◽  
High Quantum Yield ◽  
High Quantum ◽  
High Fluorescence ◽  
High Fluorescence Quantum Yield

An easily accessible fluorescent dye, FEB, which has a high fluorescence quantum yield and is suitable forin vivotumour imaging is reported.

scholarly journals Near-Infrared Markers based on Bacterial Phytochromes with Phycocyanobilin as a Chromophore

2019 ◽  
Vol 20 (23) ◽  
pp. 6067 ◽  
Author(s):  
Olesya V. Stepanenko ◽  
Olga V. Stepanenko ◽  
Olesya G. Shpironok ◽  
Alexander V. Fonin ◽  
Irina M. Kuznetsova ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Cell Biology ◽  
Fluorescence Spectra ◽  
Near Infrared ◽  
Biological Tissues ◽  
Lower Mobility ◽  
High Fluorescence ◽  
Bacterial Phytochromes

Biomarkers engineered on the basis of bacterial phytochromes with biliverdin IXα (BV) cofactor as a chromophore are increasingly used in cell biology and biomedicine, since their absorption and fluorescence spectra lie within the so-called optical “transparency window” of biological tissues. However, the quantum yield of BV fluorescence in these biomarkers does not exceed 0.145. The task of generating biomarkers with a higher fluorescence quantum yield remains relevant. To address the problem, we proposed the use of phycocyanobilin (PCB) as a chromophore of biomarkers derived from bacterial phytochromes. In this work, we characterized the complexes of iRFP713 evolved from RpBphP2 and its mutant variants with different location of cysteine residues capable of covalent tetrapyrrole attachment with the PCB cofactor. All analyzed proteins assembled with PCB were shown to have a higher fluorescence quantum yield than the proteins assembled with BV. The iRFP713/V256C and iRFP713/C15S/V256C assembled with PCB have a particularly high quantum yield of 0.5 and 0.45, which exceeds the quantum yield of all currently available near-infrared biomarkers. Moreover, PCB has 4 times greater affinity for iRFP713/V256C and iRFP713/C15S/V256C proteins compared to BV. These data establish iRFP713/V256C and iRFP713/C15S/V256C assembled with the PCB chromophore as promising biomarkers for application in vivo. The analysis of the spectral properties of the tested biomarkers allowed for suggesting that the high-fluorescence quantum yield of the PCB chromophore can be attributed to the lower mobility of the D-ring of PCB compared to BV.

scholarly journals A Facile Synthesis of Highly Nitrogen-Doped Carbon Dots for Imaging and Detection in Biological Samples

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Qianchun Zhang ◽  
Siqi Xie ◽  
Yanqun Yang ◽  
Yun Wu ◽  
Xingyi Wang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Fluorescence Probe ◽  
Fluorescence Emission ◽  
Carbon Quantum Dots ◽  
Nitrogen Doped ◽  
Low Toxicity ◽  
High Fluorescence ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

A facile, green, and high-output hydrothermal synthesis was proposed for the fabrication of highly fluorescent nitrogen-doped carbon quantum dots (N-doped CDs). The nitrogen content in N-doped CDs reached 19.2% and demonstrated strong blue fluorescence emission was obtained with fluorescence quantum yield (QY) of up to 32.9%, which exhibit high fluorescence quantum yield, high photostability, and excellent biocompatibility. The N-doped CDs possess high photostability, low toxicity, and excellent biocompatibility, based on which the N-doped CDs were successfully applied as a fluorescence probe for cell imaging. Moreover, it was then successfully demonstrated for sensitive and selective detection of Fe3+ in serum.

Highly efficient ultraviolet light-emitting organosoluble polyimide

RSC Advances ◽  
2016 ◽  
Vol 6 (74) ◽  
pp. 70008-70011 ◽  
Author(s):  
Zhiyang Liu ◽  
Shouming Wu ◽  
Yongqi Bai ◽  
Weigang Jiang ◽  
Ling Hong ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Ultraviolet Light ◽  
Fluorescence Quantum Yield ◽  
Fluorescent Dyes ◽  
Light Emitting ◽  
Highly Efficient ◽  
High Fluorescence ◽  
High Fluorescence Quantum Yield

A non-conjugated siloxane-containing polyimide without fluorescent dyes exhibited a high fluorescence quantum yield.

Synthesis, properties and photo-responsive behavior of luminescent side chain polymers containing D–π-A α-cyanostilbene units

2019 ◽  
Vol 10 (21) ◽  
pp. 2706-2715 ◽  
Author(s):  
Yongjie Yuan ◽  
Lifang He ◽  
Jindi Li ◽  
Hailiang Zhang
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Side Chain ◽  
Aggregation Induced Emission ◽  
High Solid ◽  
Emission Enhancement ◽  
Synthesis Properties ◽  
Responsive Behavior ◽  
Solid Fluorescence

A series of luminescent side chain polymers with aggregation-induced emission enhancement characteristics, high solid fluorescence quantum yield, and reversible fluorescence photo-responsive properties were prepared.

scholarly journals Ultramicronized Palmitoylethanolamide (um-PEA): A New Possible Adjuvant Treatment in COVID-19 patients

2021 ◽  
Vol 14 (4) ◽  
pp. 336
Author(s):  
Annalisa Noce ◽  
Maria Albanese ◽  
Giulia Marrone ◽  
Manuela Di Lauro ◽  
Anna Pietroboni Zaitseva ◽  
...  
Keyword(s):  
Adjuvant Treatment ◽  
Pulmonary Involvement ◽  
In Vivo Studies ◽  
Pharmacological Treatments ◽  
Beneficial Effects ◽  
Coronavirus Infection ◽  
Ultramicronized Palmitoylethanolamide ◽  
Potential Use

The Coronavirus Disease-19 (COVID-19) pandemic has caused more than 100,000,000 cases of coronavirus infection in the world in just a year, of which there were 2 million deaths. Its clinical picture is characterized by pulmonary involvement that culminates, in the most severe cases, in acute respiratory distress syndrome (ARDS). However, COVID-19 affects other organs and systems, including cardiovascular, urinary, gastrointestinal, and nervous systems. Currently, unique-drug therapy is not supported by international guidelines. In this context, it is important to resort to adjuvant therapies in combination with traditional pharmacological treatments. Among natural bioactive compounds, palmitoylethanolamide (PEA) seems to have potentially beneficial effects. In fact, the Food and Drug Administration (FDA) authorized an ongoing clinical trial with ultramicronized (um)-PEA as an add-on therapy in the treatment of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. In support of this hypothesis, in vitro and in vivo studies have highlighted the immunomodulatory, anti-inflammatory, neuroprotective and pain-relieving effects of PEA, especially in its um form. The purpose of this review is to highlight the potential use of um-PEA as an adjuvant treatment in SARS-CoV-2 infection.

UV-Trained and Metal-Enhanced Fluorescence of Biliverdin and Biliverdin Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Parinaz Fathi ◽  
Ayman Roslend ◽  
Kritika Mehta ◽  
Parikshit Moitra ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Biomedical Imaging ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Increasing the fluorescence quantum yield of fluorophores is of great interest for in vitro and in vivo biomedical imaging applications. At the same time, photobleaching and photodegradation resulting from continuous...

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