scholarly journals Assessment of Aqueous Extraction Methods on Extractable Organic Matter and Hydrophobic/Hydrophilic Fractions of Virgin Forest Soils

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2480
Author(s):  
Wan Muhammad Ikram Wan Mohd Zamri ◽  
Fridelina Sjahrir ◽  
Nor Suhaila Yaacob ◽  
Noor Fazreen Dzulkafli ◽  
Mohd Fadzli Ahmad ◽  
...  
Keyword(s):  
Organic Matter ◽  
Forest Soils ◽  
Extraction Methods ◽  
Soil Extract ◽  
Nitrate Content ◽  
Soil Extraction ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Virgin Forest ◽  
Extractable Organic Matter

The assessment of water-extractable organic matter using an autoclave can provide useful information on physical, chemical, and biological changes within the soil. The present study used virgin forest soils from Chini Forest Reserve, Langkawi Island, and Kenyir Forest Reserve (Malaysia), extracted using different extraction methods. The dissolved organic carbon (DOC), total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and ammonium-nitrate content were higher in the autoclave treatments, up to 3.0, 1.3, 1.2, and 1.4 times more than by natural extraction (extracted for 24 h at room temperature). Overall, the highest extractable DOC, TDN, TDP, ammonium and nitrate could be seen under autoclaved conditions 121 °C 2×, up to 146.74 mg C/L, 8.97 mg N/L, 0.23 mg P/L, 5.43 mg N mg/L and 3.47 N mg/L, respectively. The soil extracts became slightly acidic with a higher temperature and longer duration. Similar trends were observed in the humic and nonhumic substances, where different types of soil extract treatments influenced the concentrations of the fractions. Different soil extraction methods can provide further details, thus widening the application of soil extracts, especially in microbes.

scholarly journals The Effectiveness of Soil Extracts from Selangor Peat Swamp and Pristine Forest Soils on the Growth of Green Microalgae sp.

Forests ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 79
Author(s):  
Nor Suhaila Yaacob ◽  
Mohd Fadzli Ahmad ◽  
Ashvini Sivam ◽  
Emi Fazlina Hashim ◽  
Maegala Nallapan Maniyam ◽  
...  
Keyword(s):  
Extraction Method ◽  
Mineral Soil ◽  
Extraction Methods ◽  
Soil Extract ◽  
High Rate ◽  
Growth Promoter ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Peat Swamp ◽  
Pristine Forest

Microalgae are widely utilized in commercial industries. The addition of a modified artificial medium (soil extract) could enhance their growth. Soil extract collected from the Raja Musa peat swamp and mineral soil from the Ayer Hitam Forest Reserve (AHFR), Selangor, Malaysia, were treated using various extraction methods. Carteria radiosa PHG2-A01, Neochloris conjuncta, and Nephrochlamys subsolitaria were grown in microplates at 25 °C, light intensity 33.75 µmol photons m−2s−1 for 9 days. N. conjuncta dominated the growth in 121 °C twice extraction method AFHR samples, with 47.17% increment. The highest concentrations of ammonia and nitrate were detected in the medium with soil extract treated with 121 °C twice extraction method, yielding the concentrations of 2 mg NL−1 and 35 mg NL−1 for ammonia and nitrate of RM soil and 2 mg NL−1 and 2.85 mg NL−1 for the AH soil. These extracts are proved successful as a microalgal growth stimulant, increasing revenue and the need for enriched medium. The high rate of nutrient recovery has the potential to serve as a growth promoter for microalgae.

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

Comparing NaOH-extractable organic matter of acid forest soils that differ in their pedogenic trends: a pyrolysis-GC/MS study

2011 ◽  
Vol 62 (6) ◽  
pp. 834-848 ◽  
Author(s):  
M. Suárez Abelenda ◽  
P. Buurman ◽  
M. Camps Arbestain ◽  
J. Kaal ◽  
A. Martinez-Cortizas ◽  
...  
Keyword(s):  
Organic Matter ◽  
Forest Soils ◽  
Acid Forest Soils ◽  
Extractable Organic Matter

scholarly journals Effective soil extraction method for cultivating previously uncultured soil bacteria

2018 ◽  
Author(s):  
Tuan Manh Nguyen ◽  
Chan Seo ◽  
Moongi Ji ◽  
Man-Jeong Paik ◽  
Seung-Woon Myung ◽  
...  
Keyword(s):  
Soil Bacteria ◽  
Soil Extract ◽  
Low Molecular Weight ◽  
Critical Problem ◽  
Soil Extraction ◽  
Soil Extracts ◽  
Single Cell Sequencing ◽  
Uncultured Bacteria ◽  
Solid Media ◽  
Microbial Strains

ABSTRACTHere, a new medium named as intensive soil extract medium (ISEM) based on new soil extract (NSE) using 80% ethanol was used to efficiently isolate previously uncultured bacteria and new taxonomic candidates, which accounted for 49% and 55% of the total isolates examined (n=258), respectively. The new isolates were affiliating with seven phyla such asProteobacteria, Acidobacteria, Firmicutes, Actinobacteria, Verrucomicrobia, Planctomycetes, andBacteroidetes. The result of chemical analysis showed that NSE included more diverse components of low-molecular-weight organic substances than two conventional soil extracts using distilled water. Cultivation of previously uncultured bacteria is expected to extend knowledge through the discovery of new phenotypic, physiological and functional properties, and even roles of unknown genes.IMPORTANCEEither metagenomics or single-cell sequencing can detect unknown genes from uncultured microbial strains in environments and may find their significant potential metabolites and roles. However, such gene/genome-based techniques still have a critical problem making impossible for further applications through cultivation. To solve this problem, various approaches for cultivation of uncultured bacteria have been developed, but they still have lack of skill to grow them on solid media for isolation and subculture.

scholarly journals Effective Soil Extraction Method for Cultivating Previously Uncultured Soil Bacteria

2018 ◽  
Vol 84 (24) ◽  
Author(s):  
Tuan Manh Nguyen ◽  
Chan Seo ◽  
Moongi Ji ◽  
Man-Jeong Paik ◽  
Seung-Woon Myung ◽  
...  
Keyword(s):  
Soil Bacteria ◽  
Soil Extract ◽  
Low Molecular Weight ◽  
Organic Substances ◽  
Soil Extraction ◽  
Soil Extracts ◽  
Single Cell Sequencing ◽  
Uncultured Bacteria ◽  
Pure Cultures ◽  
Microbial Strains

ABSTRACTHere, a new medium, named intensive soil extract medium (ISEM), based on new soil extract (NSE) using 80% methanol, was used to efficiently isolate previously uncultured bacteria and new taxonomic candidates, which accounted for 49% and 55% of the total isolates examined (n= 258), respectively. The new isolates were affiliated with seven phyla (Proteobacteria,Acidobacteria,Firmicutes,Actinobacteria,Verrucomicrobia,Planctomycetes, andBacteroidetes). The result of chemical analysis showed that NSE included more diverse components of low-molecular-weight organic substances than two conventional soil extracts made using distilled water. Cultivation of previously uncultured bacteria is expected to extend knowledge through the discovery of new phenotypic, physiological, and functional properties and even roles of unknown genes.IMPORTANCEBoth metagenomics and single-cell sequencing can detect unknown genes from uncultured microbial strains in environments, and either method may find the significant potential metabolites and roles of these strains. However, such gene/genome-based techniques do not allow detailed investigations that are possible with cultures. To solve this problem, various approaches for cultivation of uncultured bacteria have been developed, but there are still difficulties in maintaining pure cultures by subculture.

Automated Analysis of Soil Extracts for Phosphorus, Potassium, Calcium, and Magnesium

1980 ◽  
Vol 63 (4) ◽  
pp. 779-787 ◽  
Author(s):  
Roy L Flannery ◽  
Denes K Markus
Keyword(s):  
Atomic Absorption ◽  
Ammonium Acetate ◽  
Sandy Loam ◽  
Acid Soil ◽  
Extraction Methods ◽  
Soil Extraction ◽  
Soil Extracts ◽  
Loam Soil ◽  
Bray P1 ◽  
Acid Extractant

Abstract An automated method is described for determining P in double acid (0.05N HCI + 0.025N H2SO4) and Bray P1 (0.025N HCI + 0.03N NH4F) soil extracts and K, Ca, and Mg in double acid and 1N ammonium acetate soil extracts. Comparisons are made between double acid and Bray P1 soil extraction methods for removal of P from a Freehold sandy loam soil that had been differentially fertilized with P fertilizers. Comparisons are also made between the double acid and ammonium acetate soil extraction methods for removal of K, Ca, and Mg from the same soil that had been differentially fertilized and limed with K fertilizers and pulverized–dolomitic limestone, respectively. Comparisons are made between automated and atomic absorption determinations of K, Ca, and Mg in standard solutions and double acid and ammonium acetate soil extracts. Bray P1 soil extractant removed 30–73% more P than did the double acid soil extractant from Freehold sandy loam soil. Ammonium acetate extractant removed about 25% more K from soil than did the double acid extractant. Double acid soil extractant removed more Ca from the soil than did ammonium acetate extractant, particularly at high soil Ca levels. Greater amounts of Ca were found in both types of soil extracts by the automated than by atomic absorption techniques. Double acid extractant removed more Mg from soil than did ammonium acetate extractant. The 2 instrumentation methods produced similar results for Mg and K analyses of both soil extract types.

scholarly journals Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 653
Author(s):  
Nor Suhaila Yaacob ◽  
Mohd Fadzli Ahmad ◽  
Nobuyuki Kawasaki ◽  
Maegala Nallapan Maniyam ◽  
Hasdianty Abdullah ◽  
...  
Keyword(s):  
Synthetic Medium ◽  
Extraction Methods ◽  
Peninsular Malaysia ◽  
Growth Promoter ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Dissolved Phosphorus ◽  
Total Dissolved Nitrogen ◽  
Medium Mass ◽  
The Cost

Soil extracts are useful nutrients to enhance the growth of microalgae. Therefore, the present study attempts for the use of virgin soils from Peninsular Malaysia as growth enhancer. Soils collected from Raja Musa Forest Reserve (RMFR) and Ayer Hitam Forest Reserve (AHFR) were treated using different extraction methods. The total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and dissolved organic carbon (DOC) concentrations in the autoclave methods were relatively higher than natural extraction with up to 132.0 mg N/L, 10.7 mg P/L, and 2629 mg C/L, respectively for RMFR. The results of TDN, TDP, and DOC suggested that the best extraction methods are autoclaved at 121 °C twice with increasing 87%, 84%, and 95%, respectively. Chlorella vulgaris TRG 4C dominated the growth at 121 °C twice extraction method in the RMRF and AHRF samples, with increasing 54.3% and 14%, respectively. The specific growth rate (µ) of both microalgae were relatively higher, 0.23 d−1 in the Ayer Hitam Soil. This extract served well as a microalgal growth promoter, reducing the cost and the needs for synthetic medium. Mass production of microalgae as aquatic feed will be attempted eventually. The high recovery rate of nutrients has a huge potential to serve as a growth promoter for microalgae.

Sign in / Sign up

Export Citation Format

Share Document