scholarly journals Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 653
Author(s):  
Nor Suhaila Yaacob ◽  
Mohd Fadzli Ahmad ◽  
Nobuyuki Kawasaki ◽  
Maegala Nallapan Maniyam ◽  
Hasdianty Abdullah ◽  
...  
Keyword(s):  
Synthetic Medium ◽  
Extraction Methods ◽  
Peninsular Malaysia ◽  
Growth Promoter ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Dissolved Phosphorus ◽  
Total Dissolved Nitrogen ◽  
Medium Mass ◽  
The Cost

Soil extracts are useful nutrients to enhance the growth of microalgae. Therefore, the present study attempts for the use of virgin soils from Peninsular Malaysia as growth enhancer. Soils collected from Raja Musa Forest Reserve (RMFR) and Ayer Hitam Forest Reserve (AHFR) were treated using different extraction methods. The total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and dissolved organic carbon (DOC) concentrations in the autoclave methods were relatively higher than natural extraction with up to 132.0 mg N/L, 10.7 mg P/L, and 2629 mg C/L, respectively for RMFR. The results of TDN, TDP, and DOC suggested that the best extraction methods are autoclaved at 121 °C twice with increasing 87%, 84%, and 95%, respectively. Chlorella vulgaris TRG 4C dominated the growth at 121 °C twice extraction method in the RMRF and AHRF samples, with increasing 54.3% and 14%, respectively. The specific growth rate (µ) of both microalgae were relatively higher, 0.23 d−1 in the Ayer Hitam Soil. This extract served well as a microalgal growth promoter, reducing the cost and the needs for synthetic medium. Mass production of microalgae as aquatic feed will be attempted eventually. The high recovery rate of nutrients has a huge potential to serve as a growth promoter for microalgae.

scholarly journals The Effectiveness of Soil Extracts from Selangor Peat Swamp and Pristine Forest Soils on the Growth of Green Microalgae sp.

Forests ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 79
Author(s):  
Nor Suhaila Yaacob ◽  
Mohd Fadzli Ahmad ◽  
Ashvini Sivam ◽  
Emi Fazlina Hashim ◽  
Maegala Nallapan Maniyam ◽  
...  
Keyword(s):  
Extraction Method ◽  
Mineral Soil ◽  
Extraction Methods ◽  
Soil Extract ◽  
High Rate ◽  
Growth Promoter ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Peat Swamp ◽  
Pristine Forest

Microalgae are widely utilized in commercial industries. The addition of a modified artificial medium (soil extract) could enhance their growth. Soil extract collected from the Raja Musa peat swamp and mineral soil from the Ayer Hitam Forest Reserve (AHFR), Selangor, Malaysia, were treated using various extraction methods. Carteria radiosa PHG2-A01, Neochloris conjuncta, and Nephrochlamys subsolitaria were grown in microplates at 25 °C, light intensity 33.75 µmol photons m−2s−1 for 9 days. N. conjuncta dominated the growth in 121 °C twice extraction method AFHR samples, with 47.17% increment. The highest concentrations of ammonia and nitrate were detected in the medium with soil extract treated with 121 °C twice extraction method, yielding the concentrations of 2 mg NL−1 and 35 mg NL−1 for ammonia and nitrate of RM soil and 2 mg NL−1 and 2.85 mg NL−1 for the AH soil. These extracts are proved successful as a microalgal growth stimulant, increasing revenue and the need for enriched medium. The high rate of nutrient recovery has the potential to serve as a growth promoter for microalgae.

scholarly journals Assessment of Aqueous Extraction Methods on Extractable Organic Matter and Hydrophobic/Hydrophilic Fractions of Virgin Forest Soils

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2480
Author(s):  
Wan Muhammad Ikram Wan Mohd Zamri ◽  
Fridelina Sjahrir ◽  
Nor Suhaila Yaacob ◽  
Noor Fazreen Dzulkafli ◽  
Mohd Fadzli Ahmad ◽  
...  
Keyword(s):  
Organic Matter ◽  
Forest Soils ◽  
Extraction Methods ◽  
Soil Extract ◽  
Nitrate Content ◽  
Soil Extraction ◽  
Forest Reserve ◽  
Soil Extracts ◽  
Virgin Forest ◽  
Extractable Organic Matter

The assessment of water-extractable organic matter using an autoclave can provide useful information on physical, chemical, and biological changes within the soil. The present study used virgin forest soils from Chini Forest Reserve, Langkawi Island, and Kenyir Forest Reserve (Malaysia), extracted using different extraction methods. The dissolved organic carbon (DOC), total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and ammonium-nitrate content were higher in the autoclave treatments, up to 3.0, 1.3, 1.2, and 1.4 times more than by natural extraction (extracted for 24 h at room temperature). Overall, the highest extractable DOC, TDN, TDP, ammonium and nitrate could be seen under autoclaved conditions 121 °C 2×, up to 146.74 mg C/L, 8.97 mg N/L, 0.23 mg P/L, 5.43 mg N mg/L and 3.47 N mg/L, respectively. The soil extracts became slightly acidic with a higher temperature and longer duration. Similar trends were observed in the humic and nonhumic substances, where different types of soil extract treatments influenced the concentrations of the fractions. Different soil extraction methods can provide further details, thus widening the application of soil extracts, especially in microbes.

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

scholarly journals Notes on the Hoya (Apocynaceae, Asclepiadoideae) from Pergau Forest Reserve, Kelantan, Peninsular Malaysia

2020 ◽  
Vol 549 ◽  
pp. 012031
Author(s):  
Rahmad Zakaria ◽  
Azim Rabbani ◽  
Syed Mohd Edhzam Syed Hamzah ◽  
Zulhazman Hamzah ◽  
Muhammad Faizal
Keyword(s):  
Peninsular Malaysia ◽  
Forest Reserve

Liquid Chromatographic Method for Determination of Extractable Nitroaromatic and Nitramine Residues in Soil

1989 ◽  
Vol 72 (6) ◽  
pp. 890-899 ◽  
Author(s):  
Thomas F Jenkins ◽  
Mariannee Walsh ◽  
Patricia W Schumacher ◽  
Paul H Miyares ◽  
Christopher F Bauer ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Extraction Methods ◽  
Ease Of Use ◽  
Linear Calibration ◽  
Soil Extracts ◽  
Spiked Soil ◽  
Collaborative Test ◽  
Wide Range ◽  
Working Standards ◽  
Liquid Chromatographic

Abstract An analytical method was developed to determine the concentration of nitroaromatic and nitramine residues in soil. Air-dried soil samples are ground with a mortar and pestle and extracted with acetonitrile in an ultrasonic bath. A portion of extract is diluted with aqueous CaCh to flocculate suspended particles, filtered, and analyzed by liquid chromatography. The method provides linear calibration curves over a wide range of concentration. Detection limits ranged from 0.03 to 1.27 ng/g. Recovery of spiked analyte was better than 80% for all analytes tested. Each step in the analytical procedure was optimized using spiked and field-contaminated soils. This optimization included tests to (1) assess the effectiveness and kinetics associated with various extraction methods, solvents, and soil-to-solvent ratios; (2) compare separations achievable using various combinations of reverse-phase columns and eluants; (3) assess analyte recovery and ease of use for various procedures to remove particles from extracts; and (4) document stability of soil extracts and analytical stock and working standards. A ruggedness test and a preliminary assessment at 2 other laboratories indicated that the method was sufficiently rugged to justify a full-scale collaborative test. A comparison of extraction kinetics for spiked soil vs field-contaminated soil revealed very different kinetic behavior, indicating it is unwise to develop extraction procedures based solely on spiked soils.

Distributions of particulate and dissolved phosphorus in aquatic habitats of Peninsular Malaysia

2018 ◽  
Vol 128 ◽  
pp. 415-427 ◽  
Author(s):  
Joon Hai Lim ◽  
Choon Weng Lee ◽  
Chui Wei Bong ◽  
Yang Amri Affendi ◽  
Yii Siang Hii ◽  
...  
Keyword(s):  
Peninsular Malaysia ◽  
Aquatic Habitats ◽  
Dissolved Phosphorus

scholarly journals Spatial analysis of early mangrove regeneration in the Matang Mangrove Forest Reserve, Peninsular Malaysia, using geomatics

2020 ◽  
Vol 472 ◽  
pp. 118213
Author(s):  
Viviana Otero ◽  
Richard Lucas ◽  
Ruben Van De Kerchove ◽  
Behara Satyanarayana ◽  
Husain Mohd-Lokman ◽  
...  
Keyword(s):  
Spatial Analysis ◽  
Mangrove Forest ◽  
Peninsular Malaysia ◽  
Forest Reserve

scholarly journals Distributions and stoichiometry of dissolved nitrogen and phosphorus in the iron-fertilized region near Kerguelen (Southern Ocean)

2015 ◽  
Vol 12 (2) ◽  
pp. 623-635 ◽  
Author(s):  
S. Blain ◽  
J. Capparos ◽  
A. Guéneuguès ◽  
I. Obernosterer ◽  
L. Oriol
Keyword(s):  
Southern Ocean ◽  
Organic Nitrogen ◽  
Organic Phosphorus ◽  
Polar Front ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Species ◽  
Dissolved Phosphorus ◽  
Dissolved Nitrogen ◽  
Total Dissolved Nitrogen ◽  
Preferential Uptake

Abstract. During KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2), we determined dissolved inorganic and organic nitrogen and phosphorus species in the naturally fertilized region of Kerguelen Island (Southern Ocean). Above 150 m, stations were clearly separated by the polar front (PF), with concentrations of NO3-, NO2- and PO43- overall lower north of the PF than south. Though less pronounced, a similar trend was detectable for dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP). At all stations offshore and above the plateau, a subsurface maximum of NH4+ was observed between 50 and 150 m. We examined nutrient stoichiometry by calculating the linear combination N* = [NO3-]-16 [PO43-]. The majority of stations and depths revealed N* close to −3 μM; however, for surface waters north of the PF, N* increased up to 6 μM. This suggests a preferential uptake of PO43- versus NO3- by fast-growing diatoms. Using the tracer TNxs = [TDN]-16[TDP] (TDN, total dissolved nitrogen; TDP, total dissolved phosphorus) revealed that the dissolved organic fraction significantly contributed to changes in TNxs. TNxs values were negative for most stations and depths, and relatively constant in the 0–500 m layer. As for N*, the stations north of the PF had higher TNxs in the 0–100 m layer. We discuss this stoichiometric anomaly with respect to possible external sources and sinks of N and P. Additional data collected in February 2013 at two sites revealed the occurrence of a subsurface minimum of N* located just below the pycnocline, which denotes a layer where remineralization of particulate organic matter with low N : P ratio P, possibly associated with preferential remineralization of P versus N, persists throughout the season.

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