scholarly journals Biosensing Membrane Base on Ferulic Acid and Glucose Oxidase for an Amperometric Glucose Biosensor

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3757
Author(s):  
Gabriela Valdés-Ramírez ◽  
Laura Galicia
Keyword(s):  
Ferulic Acid ◽  
Glucose Oxidase ◽  
Aqueous Media ◽  
Glucose Biosensor ◽  
Single Step ◽  
Coefficient Of Determination ◽  
The Novel ◽  
Carbon Paste ◽  
Glucose Response ◽  
Glucose Biosensors

A biosensing membrane base on ferulic acid and glucose oxidase is synthesized onto a carbon paste electrode by electropolymerization via cyclic voltammetry in aqueous media at neutral pH at a single step. The developed biosensors exhibit a linear response from 0.082 to 34 mM glucose concentration, with a coefficient of determination R2 equal to 0.997. The biosensors display a sensitivity of 1.1 μAmM−1 cm−2, a detection limit of 0.025 mM, and 0.082 mM as glucose quantification limit. The studies reveal stable, repeatable, and reproducible biosensors response. The results indicate that the novel poly-ferulic acid membrane synthesized by electropolymerization is a promising method for glucose oxidase immobilization towards the development of glucose biosensors. The developed glucose biosensors exhibit a broader linear glucose response than other polymer-based glucose biosensors.

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached l -glycine

2017 ◽  
Vol 64 (5) ◽  
pp. 745-753 ◽  
Author(s):  
Soner Donmez ◽  
Fatma Arslan ◽  
Nurşen Sarı ◽  
Elvan Hasanoğlu Özkan ◽  
Halit Arslan
Keyword(s):  
Glucose Oxidase ◽  
Carbon Paste Electrode ◽  
Glucose Biosensor ◽  
Carbon Paste ◽  
Paste Electrode

scholarly journals Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Sabriye Yusan ◽  
Mokhlesur M. Rahman ◽  
Nasir Mohamad ◽  
Tengku M. Arrif ◽  
Ahmad Zubaidi A. Latif ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Ph Value ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Calibration Plot ◽  
Carbon Paste ◽  
Linear Calibration ◽  
Effects Of Ph ◽  
Paste Electrode ◽  
Mcm 41

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.

Evaluation of chrono-impedance technique as transduction method for a carbon paste/glucose oxidase (CP/GOx) based glucose biosensor

2010 ◽  
Vol 26 (4) ◽  
pp. 1239-1244 ◽  
Author(s):  
Carmen C. Mayorga Martinez ◽  
Ernesto F. Treo ◽  
Rossana E. Madrid ◽  
Carmelo C. Felice
Keyword(s):  
Glucose Oxidase ◽  
Glucose Biosensor ◽  
Carbon Paste ◽  
Impedance Technique

scholarly journals Advanced Ga2O3/Lignin and ZrO2/Lignin Hybrid Microplatforms for Glucose Oxidase Immobilization: Evaluation of Biosensing Properties by Catalytic Glucose Oxidation

Catalysts ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1044
Author(s):  
Artur Jędrzak ◽  
Tomasz Rębiś ◽  
Maria Kuznowicz ◽  
Agnieszka Kołodziejczak-Radzimska ◽  
Jakub Zdarta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Glucose Oxidase ◽  
Glucose Biosensor ◽  
Carbon Paste ◽  
Electrochemical Tests ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Biosensor System ◽  
Ir Transmission

In this study, novel Ga2O3/lignin and ZrO2/lignin hybrid materials were obtained and used as supports for the adsorption of the enzyme glucose oxidase (GOx). A biosensor system based on the hybrid supports was then designed to determine the concentration of glucose in various solutions. The obtained bioinspired platforms were analyzed to determine chemical and physical properties of the support structures. A determination was made of the effectiveness of the proposed method of immobilization and the quality of operation of the constructed glucose biosensor in electrochemical tests. To characterize the materials, Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA), electrokinetic (zeta) potential measurements, atomic force microscopy (AFM), particle size measurements (NIBS technique), and elemental analysis (EA) were used. In further research, glucose oxidase (GOx) was immobilized on the surface of the obtained functional Ga2O3/lignin and ZrO2/lignin biomaterials. The best immobilization capacities—24.7 and 27.1 mg g−1 for Ga2O3/lignin and ZrO2/lignin, respectively—were achieved after a 24 h immobilization process. The Ga2O3/Lig/GOx and ZrO2/Lig/GOx systems were used for the construction of electrochemical biosensor systems, in a dedicated carbon paste electrode (CPE) with the addition of graphite and ferrocene.

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