Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials

Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP–OES and to compare the effect on cell survival of human gingival fibroblasts of a conventional and a bioactive resin. Five commercially available restorative materials were employed for this study: ActivaTM Bioactive Restorative, ENA HRi, Enamel plus HRi Biofunction, Fuji II LC Capsule, and Fuji IX Capsule. Disks that were polymerized with a curing LED light or left to set were immersed in: 1mL methanol or artificial saliva for GC/MS analysis, 5mL deionized water for ICP–OES, and 5mL of culture medium for cell viability. Cell viability was investigated with a modified staining sulforhodamine B assay.The following organic substances were detected: ACP, BHT, BPA, 1,4-BDDMA, CQ, DBP, DMABEE, HEMA, MCE, MeHQ, MOPA, MS, TMPTMA, and TPSb and the ions silicon, aluminum, calcium, sodium, and barium. Activa Bioactive Restorative was found to be biocompatible. Elution of organic substances depended on material’s composition, the nature of the solvent and the storage time. Ions’ release depended on material’s composition and storage time. The newly introduced bioactive restorative was found to be more biocompatible.

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Evaluation of color stability of different temporary restorative materials

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.0017 ◽

2015 ◽

Vol 44 (5) ◽

pp. 262-267 ◽

Cited By ~ 1

Author(s):

José Vitor Quinelli Mazaro ◽

Luiz Miguel Minani ◽

Adriana Cristina Zavanelli ◽

Caroline Cantieri de Mello ◽

Cleidiel Aparecido Araújo Lemos

Keyword(s):

Storage Time ◽

Color Change ◽

Artificial Saliva ◽

Color Stability ◽

Time Interval ◽

Acrylic Resins ◽

Significant Difference ◽

Restorative Materials ◽

Before And After ◽

Level Of Significance

AbstractIntroductionTemporary restorative materials are widely used, however, little is know about their color stability.Objectiveto evaluate the color stability of the following temporary restorative materials: acrylic and bis-acrylic resins after immersion in pigmenting solutions for different periods of storage.Material and methodFour materials were tested (Dêncor/Clássico, Protemp 4/3M ESPE; Structur 2 SC/Voco; Luxatemp AM Plus/DMG) and 30 test specimens (15 mm in diameter and 2 mm thick) per material were fabricated. They were divided according to the storage medium (artificial saliva, saliva + cola type soda, and saliva + coffee) and storage time intervals (2, 5, 7 and 15 days). Color measurements were made before and after immersions, with use of a spectrophotometer, by means of the CIE L*a*b* system. The data were analyzed by the analysis of variance and the Tukey Test, at a level of significance of 5%.ResultAcrylic resin presented greater color stability in comparison with bis-acrylic resins (p<0.001). When bis-acrylic resins were compared no significant difference was observed between the resins Structur and Luxatemp (p=0.767). As regards solutions tested, coffee showed the highest color change values (p<0.001), and the longer the storage time interval, the greater was the color change in all the temporary restorative materials analyzed (p<0.001).ConclusionAcrylic resin presented greater color stability in comparison with bis-acrylic resins (p<0.001). Coffee caused the greatest color change, and immersion time was determinant in color stability of the temporary materials analyzed.

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Toxic elements in children’s crayons and colored pencils: Bioaccessibility assessment

Journal of the Serbian Chemical Society ◽

10.2298/jsc20091078d ◽

2021 ◽

pp. 78-78

Author(s):

Svetlana Djogo-Mracevic ◽

Slavica Razic ◽

Jelena Trisic ◽

Nikola Mitrovic ◽

Danijela Djukic-Cosic

Keyword(s):

Inductively Coupled Plasma ◽

Toxic Elements ◽

Artificial Saliva ◽

Total Content ◽

Reliable Data ◽

Icp Oes ◽

Inductively Coupled ◽

High Degree ◽

Total Concentrations

Crayons and colored pencils for children may contain toxic elements (TEs) exhibiting potential risk for children?s health including cognitive development, after their ingestion, through mouthing and chewing and eventually, their accumulation. The aim of this study was to determine total content of As, Pb, Cr, Cd, Ni, and Sb and estimate their bioaccessibility conducting artificial saliva extraction. Sixty samples of colored pencils and crayons from 10 manufacturers were analyzed. Microwave acid assisted digestion followed by inductively coupled plasma optical spectroscopy (ICP-OES) was performed for determination of total content of TE. Simulation of extraction by artificial saliva was applied to get more reliable data when bioavailability is concerned. The total concentrations of TE were higher in colored pencils than in crayons and their maximum levels were: 5.78, 9.36, 9.97, 0.615, and 6.63 mg kg-1 for As, Pb, Cr, Cd, and Ni respectively. Concentration of Sb was below the detection limit for all investigated samples. This study showed that concentration of As and Pb in several samples did not comply with European Union regulative. Bioaccessibility study showed the high degree of leaching of Cr and As from pencils, but regardless of extracted portions, concentrations of selected investigated TE were below allowed levels.

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