scholarly journals 1H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6766
Author(s):  
Martina Vermathen ◽  
Hendrik von Tengg-Kobligk ◽  
Martin Nils Hungerbühler ◽  
Peter Vermathen ◽  
Nico Ruprecht
Keyword(s):  
Lung Cancer ◽  
Cell Lines ◽  
Resistant Cell ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Advanced Lung Cancer ◽  
Magic Angle ◽  
Metabolic Resistance ◽  
Metabolic Adaptations ◽  
Nmr Study

Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is still a major ongoing problem and its investigation is of paramount interest. Here, a high-resolution magic angle spinning (HR-MAS) NMR study is presented deciphering the metabolic profile of non-small cell lung cancer (NSCLC) cells and metabolic adaptations at different levels of induced cisPt-resistance, as well as in their de-induced counterparts (cells cultivated in absence of cisPt). In total, fifty-three metabolites were identified and quantified in the 1H-HR-MAS NMR cell spectra. Metabolic adaptations to cisPt-resistance were detected, which correlated with the degree of resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations as the corresponding cisPt-resistant cell lines. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione and taurine. Characteristic metabolic patterns for cisPt resistance may become relevant as biomarkers in cancer medicine.

scholarly journals 1H HR-MAS NMR Spectroscopy and the Metabolite Determination of Typical Foods in Mediterranean Diet

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Carmelo Corsaro ◽  
Domenico Mallamace ◽  
Sebastiano Vasi ◽  
Vincenzo Ferrantelli ◽  
Giacomo Dugo ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Mediterranean Diet ◽  
Food Product ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
Protected Designation Of Origin ◽  
Nmr Study ◽  
Vegetable Foods ◽  
Angle Spinning

NMR spectroscopy has become an experimental technique widely used in food science. The experimental procedures that allow precise and quantitative analysis on different foods are relatively simple. For a better sensitivity and resolution, NMR spectroscopy is usually applied to liquid sample by means of extraction procedures that can be addressed to the observation of particular compounds. For the study of semisolid systems such as intact tissues, High-Resolution Magic Angle Spinning (HR-MAS) has received great attention within the biomedical area and beyond. Metabolic profiling and metabolism changes can be investigated both in animal organs and in foods. In this work we present a proton HR-MAS NMR study on the typical vegetable foods of Mediterranean diet such as the Protected Geographical Indication (PGI) cherry tomato of Pachino, the PGI Interdonato lemon of Messina, several Protected Designation of Origin (PDO) extra virgin olive oils from Sicily, and the Traditional Italian Food Product (PAT) red garlic of Nubia. We were able to identify and quantify the main metabolites within the studied systems that can be used for their characterization and authentication.

A multinuclear MAS NMR study of the short-range structure of fluorophosphate glass

1992 ◽  
Vol 7 (7) ◽  
pp. 1892-1899 ◽  
Author(s):  
R.K. Brow ◽  
Z.A. Osborne ◽  
R.J. Kirkpatrick
Keyword(s):  
Nmr Spectra ◽  
Chemical Shifts ◽  
Spectroscopic Studies ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
Nmr Studies ◽  
Nmr Study ◽  
Fluorophosphate Glass ◽  
Angle Spinning

We have examined the bonding arrangements in Na–P–O–F and Na–Al–P–O–F glasses using 19F, 27Al, and 31P solid-state magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. For the Al-free series of glasses, the 19F NMR spectra are dominated by peaks near +90 ppm, representative of F terminating P-chains. The formation of these bonds has little effect on the 31P chemical shifts, indicating that F preferentially replaces bridging oxygen on the phosphate tetrahedra, consistent with previous NMR studies of crystalline fluorophosphates and other spectroscopic studies of fluorophosphate glass. For the Na–Al–P–O–F glasses, 27Al NMR detects only octahedral Al-sites, the 19F NMR spectra include a second peak near −12 ppm due to F bonded to Al, and the 31P NMR spectra contain signals due to Q1-sites with one or more Al next-nearest neighbors. The relative intensity of the two 19F peaks correlates well with previous spectroscopic studies and shows that a greater fraction of F–P bonds forms when the base glass is remelted in NH4HF2.

95Mo NMR Study of Crystallization in Model Nuclear Waste Glasses

2008 ◽  
Vol 1124 ◽  
Author(s):  
Scott Kroeker ◽  
Ian Farnan ◽  
Sophie Schuller ◽  
Thierry Advocat
Keyword(s):  
Nuclear Waste ◽  
Spectral Characteristics ◽  
Sodium Molybdate ◽  
Local Environment ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Sharp Peak ◽  
Magic Angle ◽  
Borosilicate Glasses ◽  
Nmr Study

Abstract95Mo magic-angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy is surprisingly sensitive to the local environment of tetrahedral molybdate species. A series of compounds related to expected crystallization products in nuclear waste glasses are probed to calibrate their spectral characteristics. Glasses formed with fast and slow quenching show a glassy peak corresponding to tetrahedral molybdate species. With slow quenching, a prominent sharp peak is observed, representing crystallinity. In sodium-borosilicate glasses with 2.5 mol% MoO3, the sharp peak corresponds to pure crystalline sodium molybdate. Cesium-sodium and lithium-sodium borosilicate glasses with Mo show crystalline peaks as well, and suggest that NMR may potentially be used to characterize mixed-cation molydates and more complex phase assemblages. While precise quantification of Mo in different phases is likely to be time-consuming, reasonable estimates can be obtained routinely, making 95Mo MAS NMR a useful tool for investigating phase separation and crystallization in model nuclear waste materials.

Distribution of water in the pores of periodic mesoporous organosilicates – a proton solid state MAS NMR study

2018 ◽  
Vol 20 (46) ◽  
pp. 29351-29361 ◽  
Author(s):  
V. S. Veena ◽  
Kavya Illath ◽  
Anish Lazar ◽  
C. P. Vinod ◽  
T. G. Ajithkumar ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectra ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
Pore Filling ◽  
Nmr Study ◽  
Proposed Model ◽  
Water Layers ◽  
Angle Spinning

Proposed model of water layers and pore filling in ethane substituted periodic mesoporous organosilicates (PMOE) based on analysis of solid state magic angle spinning (MAS) proton NMR spectra.

29Si and 27Al MAS NMR study of the thermal transformations of kaolinite from North China

Clay Minerals ◽  
2003 ◽  
Vol 38 (4) ◽  
pp. 551-559 ◽  
Author(s):  
H. P. He ◽  
J . G. Guo ◽  
J . X. Zhu ◽  
C. Hu
Keyword(s):  
Nmr Spectra ◽  
Thermal Transformation ◽  
Carbonaceous Material ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
Thermal Transformations ◽  
Nmr Study ◽  
27Al Mas Nmr ◽  
Angle Spinning

AbstractThe thermal transformations of kaolinite have been studied using 27Al and 29Si magic angle spinning nuclear magnetic resonance (MAS NMR), X-ray diffraction (XRD), differential thermal analysis (DTA) and thermogravimetric analysis (TG). The experimental results show that this sample is a pure kaolinite which contains ∼3% carbonaceous material as impurity. 27Al and 29Si MAS NMR spectra show that the microstructural evolution of the thermal transformation of kaolinite at 450 –1050ºC is similar to that of the kaolinite– mullite reaction series previously published by many authors. 29Si MAS NMR spectra of mullite at 1190 and 1250ºC display two resonances at ∼ – 87 and –92 ppm, corresponding to sillimanite-type geometry around Si and the typical Si environment of mullite, respectively. At 1350ºC, the splitting of (hk0) and (kh0) of mullite occurs, indicating that the primary mullite transforms into orthorhombic mullite. Simultaneously, the resonance at ∼ – 92 ppm splits into two signals at ∼ –90 and –94 ppm. 27Al MAS NMR spectra of the mullite consist of three signals centred at ∼ –4, 45 and 60 ppm, corresponding to octahedral, distorted tetrahedral and tetrahedral Al sites, respectively.

scholarly journals The MAS NMR study of solid solutions based on the YAG crystal

Nukleonika ◽  
2015 ◽  
Vol 60 (3) ◽  
pp. 417-421 ◽  
Author(s):  
Bohdan V. Padlyak ◽  
Nikolaj A. Sergeev ◽  
Marcin Olszewski ◽  
Piotr Stępień
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Solid Solutions ◽  
Yttrium Aluminum Garnet ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
Nmr Study ◽  
Yttrium Aluminum ◽  
Angle Spinning

Abstract An 27Al magic angle spinning (MAS) nuclear magnetic resonance (NMR) study of nominally pure and Cr-doped yttrium-aluminum garnet (Y3Al5O12 and Y3Al5O12:Cr) crystals is reported. It has been shown that the doping by Cr of the Y3Al5O12 crystals leads to the variation of the occupation by Al atoms both octahedrally and tetrahedrally coordinated sites of the garnet lattice.

MAS NMR Study on the Microstructural Changes of Zeolite-NaY under Neutron Irradiation

2003 ◽  
Vol 792 ◽  
Author(s):  
J. Chen ◽  
L. W. Beck ◽  
L. M. Wang ◽  
B. X. Gu ◽  
R. C. Ewing
Keyword(s):  
Ion Exchange ◽  
Neutron Irradiation ◽  
Dose Range ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Irradiation Damage ◽  
Magic Angle ◽  
Bond Angles ◽  
Zeolite Nay ◽  
Nmr Study

ABSTRACTThe results of high-resolution solid-state magic angle spinning nuclear magnetic resonance (MAS NMR) indicate the bond angles of Al-O-Si decrease with the increase of neutronirradiation dose, but there are only minor changes for Si-O-Si bond angles in the current dose range studied. This suggests that zeolite with higher Si/Al ratio is more resistant to neutron irradiation damage. It is also found that [AlO6] formed at higher dose range. The ratio of Si/Al changed from 2.57 before neutron irradiation to 2.76 when the dose reached 2.25×1019 n/cm2, which shows that mild dealumination occurred during neutron irradiation. The strontium ion exchange experiments have been conducted for neutron irradiated, thermally treated and original zeolite samples, respectively. It is found that the ion-exchange ability of neutron-irradiated zeolite-NaY is between those of original and heat-treated zeolite samples. The distortion of the framework under neutron irradiation by the decrease of Al-O-Si bond angles and the formation of [AlO6] is partially responsible for the ion-exchange variability variation of zeolite-NaY.

Magic Angle Spinning NMR Study on Inversion Behavior and Vacancy Disorder in Alumina-Rich Spinel

2018 ◽  
Vol 57 (14) ◽  
pp. 8390-8395 ◽  
Author(s):  
Bingtian Tu ◽  
He Zhang ◽  
Hao Wang ◽  
Weimin Wang ◽  
Zhengyi Fu
Keyword(s):  
Magic Angle Spinning ◽  
Magic Angle ◽  
Nmr Study ◽  
Angle Spinning

scholarly journals Combination of solid state NMR and DFT calculation to elucidate the state of sodium in hard carbon electrodes

2016 ◽  
Vol 4 (34) ◽  
pp. 13183-13193 ◽  
Author(s):  
Ryohei Morita ◽  
Kazuma Gotoh ◽  
Mika Fukunishi ◽  
Kei Kubota ◽  
Shinichi Komaba ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Dft Calculation ◽  
Magic Angle Spinning ◽  
The State ◽  
Mas Nmr ◽  
Magic Angle ◽  
Multiple Quantum ◽  
Hard Carbon ◽  
Angle Spinning

We examined the state of sodium electrochemically inserted in HC prepared at 700–2000 °C using solid state Na magic angle spinning (MAS) NMR and multiple quantum (MQ) MAS NMR.

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