scholarly journals Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 14
Author(s):  
Nisar Ahmad ◽  
Palwasha Khan ◽  
Abdullah Khan ◽  
Maliha Usman ◽  
Mohammad Ali ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Adventitious Root ◽  
Total Phenolics ◽  
Culture Media ◽  
Stevia Rebaudiana ◽  
Biomass Accumulation ◽  
Total Flavonoids ◽  
Root Cultures ◽  
Adventitious Root Cultures ◽  
Cuscuta Reflexa

Stevia rebaudiana is an important medicinal plant that belongs to the Asteraceae family. The leaves of Stevia rebaudiana are a rich source of many health-promoting agents such as polyphenols, flavonoids, and steviol glycoside, which play a key role in controlling obesity and diabetes. New strategies such as the elicitation of culture media are needed to enhance the productivity of active components. Herein, the Cuscuta reflexa extracts were exploited as elicitors to enhance the productivity of active components. Cuscuta reflexa is one of the parasitic plants that has the ability to elongate very fast and cover the host plant. Consequently, it may be possible that the addition of Cuscuta reflexa extracts to adventitious root cultures (ADR) of Stevia rebaudiana may elongate the root more than control cultures to produce higher quantities of the desired secondary metabolites. Therefore, the main objective of the current study was to investigate the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolite in submerged adventitious root cultures of Stevia rebaudiana. Ten different concentrations of Cuscuta reflexa were added to liquid media containing 0.5 mg/L naphthalene acetic acid (NAA). The growth kinetics of adventitious roots was investigated for a period of 49 days with an interval of 7 days. The maximum biomass accumulation (7.83 g/3 flasks) was observed on medium containing 10 mg/L extract of Cuscuta reflexa on day 49. As the concentration of extract increases in the culture media, the biomass gradually decreases after 49 days of inoculation. In this study, the higher total phenolics content (0.31 mg GAE/g-DW), total flavonoids content (0.22 mg QE/g-DW), and antioxidant activity (85.54%) were observed in 100 mg/L treated cultures. The higher concentration (100 mg/L) of Cuscuta reflexa extract considerably increased the total phenolics content (TPC), total phenolics production (TPP), total flavonoids content (TFC), total flavonoids production (TFP), total polyphenolics content (TPPC), and total polyphenolics production (TPPP). It was concluded that the extract of Cuscuta reflexa moderately improved biomass accumulation but enhanced the synthesis of phenolics, flavonoids, and antioxidant activities. Here, biomass’s independent production of secondary metabolites was observed with the addition of extract. The present study will be helpful to scale up adventitious roots culture into a bioreactor for the production of secondary metabolites rather than biomass accumulation in medicinally important Stevia rebaudiana.

scholarly journals Effect of Gibberellic Acid on Production of Biomass, Polyphenolics and Steviol Glycosides in Adventitious Root Cultures of Stevia rebaudiana (Bert.)

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 420
Author(s):  
Ashfaq Ahmad ◽  
Haider Ali ◽  
Habiba Khan ◽  
Almas Begam ◽  
Sheraz Khan ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Stevia Rebaudiana ◽  
Biomass Accumulation ◽  
Total Phenolic ◽  
Naphthalene Acetic Acid ◽  
Steviol Glycosides ◽  
Root Cultures ◽  
Adventitious Root Cultures

In current study, the effect of gibberellic acid was tested for production of biomass, polyphenolics and Steviol glycosides in adventitious root cultures of Stevia rebaudiana. Adventitious cultures were induced from the roots of in vitro grown plantlets on Murashige and Skoog (MS) medium containing combination of gibberellic acid (GA3; 0.5, 1.0, 1.5 and 2.0 mg/L) and naphthalene acetic acid (NAA; 0.5 mg/L). Initially, a known mass of inoculum roots were shifted into suspension media augmented with various GA3 concentrations. The growth behavior of adventitious roots was recorded every 3 days for a period of 30 days. Maximum biomass biosynthesis (13.12 g/flask) was noticed in exponential phase on 27th day in the suspension containing 2.0 mg/L of GA3. Other GA3 concentrations also displayed optimum patterns of biomass accumulation as compared to the control. Adventitious roots were investigated for total phenolic content (TPC) and production (TPP), total flavonoid content (TFC) and production (TFP), and 1, 1-diphenyl-2-picrylhydrazyl (DPPH)-based antioxidant potential. Maximum phenolics (TPC 9.84 mg gallic acid equivalent (GAE)/g-dry weight (DW)) and TPP (147.6 mg/L), TFC (5.12 mg Quercitin equivalent (QE)/g-DW) and TFP (76.91 mg/L) were observed in 2.0 mg/L GA3 treated cultures. The same concentration of gibberellic acid enhanced antioxidant activity (77.2%). Furthermore, maximum stevioside (7.13 mg/g-DW), rebaudioside-A (0.27 mg/g-DW) and dulcoside-A (0.001 mg/g-DW) were observed in roots exposed to 2.0 mg/L GA3. This is the first report on the application of GA3 on biomass accumulation and secondary metabolite production in S. rebaudiana. The current study will be helpful to scale up the adventitious root cultures in bioreactors for the production of biomass and pharmaceutically important secondary metabolites.

scholarly journals Enhanced Aryltetralin Lignans Production in Linum Adventi-Tious Root Cultures

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5189
Author(s):  
Michela Alfieri ◽  
Iride Mascheretti ◽  
Roméo A. Dougué Kentsop ◽  
Roberto Consonni ◽  
Franca Locatelli ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Defense ◽  
Methyl Jasmonate ◽  
Human Health ◽  
Adventitious Root ◽  
Root Cultures ◽  
Adventitious Root Cultures ◽  
First Time ◽  
Defense Molecules

Lignans are the main secondary metabolites synthetized by Linum species as plant defense molecules. They are also valuable for human health, in particular, for their potent antiviral and antineoplastic properties. In this study, the adventitious root cultures of three Linum species (L. flavum, L. mucronatum and L. dolomiticum) were developed to produce aryltetralin lignans. The effect of two elicitors, methyl jasmonate and coronatine, on aryltetralin lignans production was also evaluated. The adventitious root cultures from L. dolomiticum were obtained and analyzed for the first time and resulted as the best producer for all the aryltetralins highlighted in this system: Podophyllotoxin, 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside, the last showing a productivity of 92.6 mg/g DW. The two elicitors differently affected the production of the 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside.

scholarly journals Chemical characterization and prebiotic activity of fructo-oligosaccharides from Stevia rebaudiana (Bertoni) roots and in vitro adventitious root cultures

2016 ◽  
Vol 152 ◽  
pp. 718-725 ◽  
Author(s):  
Sheila Mara Sanches Lopes ◽  
Mariane Grigio Francisco ◽  
Bruna Higashi ◽  
Rafaela Takako Ribeiro de Almeida ◽  
Gabriela Krausová ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Chemical Characterization ◽  
Stevia Rebaudiana ◽  
Root Cultures ◽  
Prebiotic Activity ◽  
Adventitious Root Cultures ◽  
Stevia Rebaudiana Bertoni

Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures

2019 ◽  
Vol 14 (3) ◽  
pp. 381-386
Author(s):  
Xi-Hua Cui ◽  
Hosakatte Niranjana Murthy ◽  
Ji-De Zhang ◽  
Hang-Lin Song ◽  
Yin-Ji Jiang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Adventitious Root ◽  
Root Cultures ◽  
Nutritional Factors ◽  
Adventitious Root Cultures

scholarly journals Effect of sucrose on the fatty acid metabolism of adventitious root cultures in vitro of Stevia rebaudiana

2021 ◽  
Vol 10 (10) ◽  
pp. e182101018651
Author(s):  
Bruna Gabrieli Follador ◽  
Éverton da Silva Santos ◽  
José Eduardo Gonçalves ◽  
Regina Aparecida Correia Gonçalves ◽  
Arildo José Braz de Oliveira
Keyword(s):  
Fatty Acids ◽  
Adventitious Root ◽  
Unsaturated Fatty Acids ◽  
Stevia Rebaudiana ◽  
Naphthaleneacetic Acid ◽  
Root Cultures ◽  
Roller Bottle ◽  
Adventitious Root Cultures ◽  
Abiotic Stress Condition

In this study, the effect of sucrose on the neutral lipid profile of adventitious root cultures of Stevia rebaudiana was evaluated. The cultures were obtained employing a roller bottle system. In this system, Schott-type flasks were used, which contained Murashige and Skoog liquid medium at 33.3% strength (MS/3) supplemented with 30, 60, and 80 g L-1 of sucrose, respectively, and 10.7 mM 1-naphthaleneacetic acid (NAA). The spectroscopic analyzes showed that the portion of polyunsaturated fatty acids (PUFAs) was highest in roots treated with 30 g L-1 of sucrose. The spectrometric analyzes showed that the palmitic acid was found to be present in relatively higher amounts in the roots submitted to the MS/3-30 g L-1 (31.9%) and MS/3-60 g L-1 (29.5%) sucrose treatments, and lower in the treatment with MS/3-80 g L-1 (28.8%) of sucrose. Also, the treatment using 30 g L-1 of sucrose was the best for obtaining unsaturated fatty acids (UFAs) in the culture, with a relative percentage of 62.9%. Our results indicate that the MS medium that received 30 g L-1 of sucrose induced a lesser abiotic stress condition, which favored PUFAs production in the adventitious root cultures of S. rebaudiana.

scholarly journals Curcubinoyl-conjugated flavonoids from methyl jasmonate-treated wild ginseng adventitious root cultures

2021 ◽  
Author(s):  
Qing Liu ◽  
Seon Beom Kim ◽  
Yang Hee Jo ◽  
Jong Hoon Ahn ◽  
Ayman Turk ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Adventitious Root ◽  
Efficient Production ◽  
Root Cultures ◽  
Raw 264.7 Macrophages ◽  
Adventitious Root Cultures ◽  
Chemical Structures ◽  
Wild Ginseng ◽  
New Metabolites

Abstract Wild ginseng (Panax ginseng) adventitious root cultures were prepared by elicitation using methyl jasmonate and investigated further to find new secondary metabolites. Chromatographic fractionation of wild ginseng adventitious root cultures led to the isolation of eleven compounds. The chemical structures of isolated compounds were identified as four known flavanone derivatives (1–4), one new curcubinoyl derivative, jasmogin A (5) and six new curcubinoyl-flavanone conjugates, jasmoflagins A-F (6–11) by extensive spectroscopic analysis. Newly isolated curcubinoyl derivatives showed inhibitory activity against lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages. Curcubinoyl moiety is structurally similar with methyl jasmonate, which was used as an elicitor. Therefore, our present study suggested that elicitor might increase the content of active constituents and also contribute to the production of new metabolites by participation as precursors in biosynthesis. Taken together, plant cell cultures are useful not only for efficient production of active compounds but also development of new secondary metabolites.

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