Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.

Download Full-text

Nuclear DNA Content and Short-Term Survival Analysis in Carcinoma of the Pancreas: A Study Using Flow Cytometry

Recent Advances in Management of Digestive Cancers ◽

10.1007/978-4-431-68252-3_237 ◽

1993 ◽

pp. 782-784

Author(s):

Seon Hahn Kim ◽

Bum-Hwan Koo ◽

Sae Min Kim

Keyword(s):

Flow Cytometry ◽

Survival Analysis ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Short Term ◽

Term Survival ◽

Carcinoma Of The Pancreas ◽

Short Term Survival

Download Full-text

Metastatic Liver Tumors in Surgical Pathology: Impact of Contemporary Diagnostic and Therapeutic Paradigms in a Tertiary Care Center

International Journal of Surgical Pathology ◽

10.1177/10668969211022708 ◽

2021 ◽

pp. 106689692110227

Author(s):

Ayşe Armutlu ◽

Omer Saeed ◽

Romil Saxena

Keyword(s):

Primary Tumor ◽

Liver Tumors ◽

Care Center ◽

Tertiary Care ◽

Comprehensive Cancer Center ◽

Tertiary Care Center ◽

Term Survival ◽

Primary Tumor Site ◽

Metastatic Liver Tumors ◽

Metastatic Liver

We analyzed metastatic liver tumors received in the department of pathology in a tertiary care center over a 3-year period. There were 509 metastatic liver tumors; counterintuitively, there were as many resections (235 cases) as biopsies (274 cases). This unexpected finding reflects contemporaneous organ-specific paradigms for diagnosis and management of metastatic liver disease in oncologic practice, and the association of our practice with a National Cancer Institute-designated comprehensive cancer center with expertise and specialization in liver surgery. We receive a large number of resections for metastatic liver tumors because metastasectomy from a variety of primary tumors is associated with improved overall, and in many instances, disease-free, long-term survival. Metastatic colorectal carcinomas, metastatic neuroendocrine tumors, and metastatic gastrointestinal stromal tumors constituted 78% of resections because the largest body of literature and cumulative experience exists for these lesions. In contrast, breast carcinomas and pancreatic carcinomas, which are the next common metastatic liver tumors were biopsied but rarely resected, because metastasectomy is not the standard of care for these tumors. Immunohistochemistry was performed in less than a quarter of the total number of cases (23%), because the primary tumor site was known in the vast majority of cases. Of the 42 cases with unknown primary tumor, it was elucidated in 50% of the cases by immunohistochemical and clinical work-up. Of the cases with known primary tumor, immunohistochemistry was performed mostly in metastatic breast, colon, and lung carcinomas. In these cases, biomarker analyses provided additional information relevant to clinical management.

Download Full-text

Long term survival and surgical outcomes of minimally invasive liver surgery for primary and metastatic liver tumors. A 15-year experience

HPB ◽

10.1016/j.hpb.2017.02.042 ◽

2017 ◽

Vol 19 ◽

pp. S51-S52

Author(s):

I. Sucandy ◽

S.M. Cheek ◽

A. Tsung ◽

J.W. Marsh ◽

D.A. Geller

Keyword(s):

Minimally Invasive ◽

Liver Surgery ◽

Surgical Outcomes ◽

Liver Tumors ◽

Term Survival ◽

Long Term Survival ◽

Minimally Invasive Liver Surgery ◽

Metastatic Liver Tumors ◽

Metastatic Liver

Download Full-text

DNA evaluation in growth hormone producing pituitary adenomas: flow cytometry versus single cell analysis

Acta Endocrinologica ◽

10.1530/acta.0.1210317 ◽

1989 ◽

Vol 121 (3) ◽

pp. 317-321 ◽

Cited By ~ 6

Author(s):

A. L. Hulting ◽

U. Askensten ◽

B. Tribukait ◽

J. Wersäll ◽

G. Auer ◽

...

Keyword(s):

Growth Hormone ◽

Flow Cytometry ◽

Dna Content ◽

Pituitary Adenomas ◽

Single Cell Analysis ◽

Tumour Size ◽

Growth Characteristics ◽

Cell Analysis ◽

Method Show ◽

Benign Course

Abstract. DNA patterns were analysed in 26 GH-producing pituitary adenomas by flow cytometry as well as by microspectrophotometry. Twelve tumours (46%) were diploid according to both methods, whereas 5 tumours (19%) showed aneuploid DNA patterns. Nine tumours were classified differently by the two methods: flow cytometry resulted in diploidy in 2 and aneuploidy in 7 patients, whereas microspectrophotometry showed diploidy in 5 tumours, tetraploidy in 3 and aneuploidy in 1. Methodological limitations may explain the discrepancy in the results obtained by the two methods. However, both the flow cytometry and the microspectrophotometry method show the presence of aneuploid DNA patterns in GH-producing pituitary adenomas despite their benign growth characteristics and the clinically benign course of the disease. This comparative study with two methods measuring DNA content, shows that depending on the criteria used for diploidy-aneuploidy, the freqency of aneuploidy will vary. In this material of 26 GH-producing adenomas, 46% were aneuploid according to flow cytometry and 23% according to microspectrophotometric. However, no correlation to tumour size or GH levels was found with either method when patients with aneuploid and diploid tumours were compared. Therefore, no clinial significance can so far be drawn from these results.

Download Full-text