scholarly journals Optimization of Wide-Field ODMR Measurements Using Fluorescent Nanodiamonds to Improve Temperature Determination Accuracy

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2282
Author(s):  
Tamami Yanagi ◽  
Kiichi Kaminaga ◽  
Wataru Kada ◽  
Osamu Hanaizumi ◽  
Ryuji Igarashi
Keyword(s):  
Cell Biology ◽  
Nitrogen Vacancy ◽  
Wide Field ◽  
Optimal Frequency ◽  
Temperature Imaging ◽  
Temperature Determination ◽  
Field Temperature ◽  
Nitrogen Vacancy Centers ◽  
Fluorescent Nanodiamonds ◽  
Optically Detected

Fluorescent nanodiamonds containing nitrogen-vacancy centers have attracted attention as nanoprobes for temperature measurements in microenvironments, potentially enabling the measurement of intracellular temperature distributions and temporal changes. However, to date, the time resolution and accuracy of the temperature determinations using fluorescent nanodiamonds have been insufficient for wide-field fluorescence imaging. Here, we describe a method for highly accurate wide-field temperature imaging using fluorescent nanodiamonds for optically detected magnetic resonance (ODMR) measurements. We performed a Monte Carlo simulation to determine the optimal frequency sweep range for ODMR temperature determination. We then applied this sweep range to fluorescent nanodiamonds. As a result, the temperature determination accuracies were improved by a factor ~1.5. Our result paves the way for the contribution of quantum sensors to cell biology for understanding, for example, differentiation in multicellular systems.

scholarly journals Divergent effects of laser irradiation on ensembles of nitrogen-vacancy centers in bulk and nano-diamonds: implications for biosensing

2021 ◽  
Author(s):  
Domingo Olivares Postigo ◽  
Federico Gorrini ◽  
Valeria Bitonto ◽  
Johannes Ackermann ◽  
Rakshyakar Giri ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Laser Power ◽  
Surface Effects ◽  
Nitrogen Vacancy ◽  
Spin Order ◽  
Surface Areas ◽  
Nitrogen Vacancy Centers ◽  
Fluorescent Nanodiamonds ◽  
Optically Detected ◽  
Bulk Diamond

Ensembles of negatively charged nitrogen vacancy centers (NV-) in diamond have been proposed for sensing of magnetic fields and paramagnetic agents, and as a source of spin-order for the hyperpolarization of nuclei in magnetic resonance applications. To this end, strongly fluorescent nanodiamonds represent promising materials, with large surface areas and dense ensembles of NV-. However, surface effects tend to favor the less useful neutral form, the NV0 centers. Here, we study the fluorescence properties and optically detected magnetic resonance (ODMR) of NV- centers as a function of laser power in strongly fluorescent bulk diamond and in nanodiamonds obtained by nanomilling the native material. In bulk diamond, we find that increasing laser power increases ODMR contrast, consistent with a power-dependent increase in spin-polarization. Surprisingly, in nanodiamonds we observe a non-monotonic behavior, with a decrease in ODMR contrast at higher laser power that can be ascribed to more efficient NV-→NV0 photoconversion in nanodiamonds compared to bulk diamond, resulting in depletion of the NV- pool. We also studied this phenomenon in cell cultures following internalization of NDs in macrophages. Our findings show that surface effects in nanodiamonds substantially affect the NV properties and provide indications for the adjustment of experimental parameters.

scholarly journals Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yushi Nishimura ◽  
Keisuke Oshimi ◽  
Yumi Umehara ◽  
Yuka Kumon ◽  
Kazu Miyaji ◽  
...  
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Nitrogen Vacancy ◽  
Wide Field ◽  
Large Area ◽  
Photon Detectors ◽  
Nitrogen Vacancy Centers ◽  
Optically Detected ◽  
Spin Measurements ◽  
Confocal Detection

AbstractMeasuring optically detected magnetic resonance (ODMR) of diamond nitrogen vacancy centers significantly depends on the photon detectors used. We study camera-based wide-field ODMR measurements to examine the performance in thermometry by comparing the results to those of the confocal-based ODMR detection. We show that the temperature sensitivity of the camera-based measurements can be as high as that of the confocal detection and that possible artifacts of the ODMR shift are produced owing to the complexity of the camera-based measurements. Although measurements from wide-field ODMR of nanodiamonds in living cells can provide temperature precisions consistent with those of confocal detection, the technique requires the integration of rapid ODMR measurement protocols for better precisions. Our results can aid the development of camera-based real-time large-area spin-based thermometry of living cells.

scholarly journals Simultaneous imaging of magnetic field and temperature using a wide-field quantum diamond microscope

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yulei Chen ◽  
Zhonghao Li ◽  
Hao Guo ◽  
Dajin Wu ◽  
Jun Tang
Keyword(s):  
Magnetic Field ◽  
Spatial Resolution ◽  
Imaging Technique ◽  
Field Of View ◽  
Nitrogen Vacancy ◽  
Wide Field ◽  
Simultaneous Imaging ◽  
Quantum Sensing ◽  
Nitrogen Vacancy Centers

AbstractQuantum sensing based on nitrogen-vacancy centers in diamond has shown excellent properties. Combined with the imaging technique, it shows exciting practicability. Here, we demonstrate the simultaneously imaging technique of magnetic field and temperature using a wide-field quantum diamond microscope. We describe the operating principles of the diamond microscope and report its sensitivity (magnetic field ${\sim}1.8~\mu \mbox{T/Hz}^{1/2}$ ∼ 1.8 μ T/Hz 1 / 2 and temperature ${\sim}0.4~\mbox{K/Hz}^{1/2}$ ∼ 0.4 K/Hz 1 / 2 ), spatial resolution (1.3 μm), and field of view ($400 \times 300~\mu \mbox{m}^{2}$ 400 × 300 μ m 2 ). Finally, we use the microscope to obtain images of an integrated cell heater and a PCB, demonstrating its ability in the application of magnetic field and temperature simultaneously imaging at wide-field.

Wide-Field Optical Microscopy of Microwave Fields Using Nitrogen-Vacancy Centers in Diamonds

2016 ◽  
Vol 4 (7) ◽  
pp. 1075-1080 ◽  
Author(s):  
Linbo Shao ◽  
Ruishan Liu ◽  
Mian Zhang ◽  
Anna V. Shneidman ◽  
Xavier Audier ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nitrogen Vacancy ◽  
Wide Field ◽  
Nitrogen Vacancy Centers

scholarly journals Optically detected nuclear quadrupolar interaction ofN14in nitrogen-vacancy centers in diamond

2014 ◽  
Vol 89 (20) ◽  
Author(s):  
Chang S. Shin ◽  
Mark C. Butler ◽  
Hai-Jing Wang ◽  
Claudia E. Avalos ◽  
Scott J. Seltzer ◽  
...  
Keyword(s):  
Nitrogen Vacancy ◽  
Quadrupolar Interaction ◽  
Nitrogen Vacancy Centers ◽  
Optically Detected
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