scholarly journals The Study of Correlation between Serum Vitamin D3 Concentrations and HBV DNA Levels and Immune Response in Chronic Hepatitis Patients

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1114
Author(s):  
Wang-Sheng Ko ◽  
Yen-Ping Yang ◽  
Fang-Ping Shen ◽  
Mu-Chen Wu ◽  
Chia-Ju Shih ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Vitamin D3 ◽  
Peripheral Blood ◽  
Hepatitis B Surface Antigen ◽  
Serum Vitamin ◽  
Hbv Dna ◽  
Hbv Infection

Chronic hepatitis B (CHB) is a common chronic disease. Previous studies have shown a link between 25-hydroxyvitamin D3 (vitamin D3) concentration and liver disease. Hepatitis B virus (HBV) infection has been attributed to the inappropriate functioning of cell-mediated immunity. However, the effects of vitamin D3, immune cell, and HBeAg status on HBV viral load in CHB patients are still unclear. We investigated the relationship between the serum concentration of vitamin D3, percentage of immune cells in peripheral blood, and the HBV viral load of CHB patients. Sixty CHB patients were recruited, and their blood samples were collected and analyzed. Vitamin D level was measured using a chemiluminescence assay. A level of 30 ng/mL or above was defined as a vitamin D3 sufficiency. We assigned vitamin D3 status as either normal (≥30 ng/mL), insufficient (20–30 ng/mL), or deficient (<20 ng/mL). T-lymphocyte and B-lymphocyte surface markers in peripheral blood were detected using flow cytometry. The factors associated with HBV viral load were analyzed using univariate and multivariate-adjusted models. The mean serum vitamin D3 concentration in the subjects was 20.9 ± 5.6 ng/mL. Up to 88.3% of the patients were either deficient in or had insufficient vitamin D3. The gender, BMI, hepatitis B surface antigen levels, and ALT levels were significantly related to serum vitamin D3 levels. Serum vitamin D3 concentration, HBe status, HBs levels, ALT, and AST levels showed a statistically significant correlation with the HBV DNA levels. Serum vitamin D3 concentrations and hepatitis B surface antigen levels were strongly correlated with HBV DNA levels. Vitamin D3 levels were significantly associated with CD19 numbers (β:−6.2, 95% CI: −10.5). In multivariate analysis, vitamin D3 levels in the deficient and insufficient groups, and the CD8, HBeAg, and WBC counts were significantly associated with HBV DNA levels. In the immune tolerance phase of HBeAg-negative chronic HBV infection, vitamin D3 may be a modulator of immune function via CD8, CD19, and HBV DNA.

Serum hepatitis B surface antigen concentration correlates with HBV DNA level in patients with chronic hepatitis B

2010 ◽  
Vol 15 (8) ◽  
pp. 1133-1139 ◽  
Author(s):  
Tung-Hung Su ◽  
Ching-Sheng Hsu ◽  
Chi-Ling Chen ◽  
Chen-Hua Liu ◽  
Yi-Wen Huang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Serum Hepatitis ◽  
Antigen Concentration

scholarly journals Response of patients with chronic Hepatitis B in one year of treatment with Major Autohemotherapy.

2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Javier Cespedes-Suarez ◽  
Yanisley Martin-Serrano ◽  
Maria Rosa Carballosa-Peña ◽  
Diana Rosa Dager-Carballosa
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Adult Population ◽  
Antiviral Treatment ◽  
Ozone Therapy ◽  
Antigen Antibody

The Hepatitis B (HVB) is one of the most common infectious diseases in the world, it’s wide geographical distribution is a health problem, especially on the African continent, with prevalence rate of 6.1% in the adult population. Current treatment requires prolonged therapy (most cases for the rest of life) with the aim of stopping viral replication, maintaining immunological stability, preventing progression of liver disease and the most feared complications such as cirrhosis and hepatic cancer There are multiple references that point to ozone therapy as an alternative in the treatment of Hepatitis B, because of the known and demonstrated antimicrobial and immunomodulatory properties. With these premises, we performed the present clinical study that included 28 patients with positive diagnosis of chronic Hepatitis B, surface antigen (HVBs Ag) positive, antibodies against surface antigen (HVBs) negative, viral load (HVB DNA ) and transaminaseselevated. These patients with 1 year of evolution and antiviral treatment were treated with Major Autohemotherapy with protocol of 15 sessions and maintenance every 15 days to 50 mcg of concentration, initial dose of 4,000 mcg / ml up to 12,000 mcg /ml We indicate Ag HVBs, Ac HVBs, HVB Viral Loading and transaminases before starting treatment at 15 days of completion and quarterly until the year. The results showed negativization of the surface antigen, antibody positivity against the surface antigen, significant decrease of viral load to undetectable values and normal values of the transaminases demonstrating the functional recovery of the disease associated with favorable immunological response providing a better quality of patients' lives. Key words: Hepatitis B, Transaminases, Ozone therapy, Major Autohemotherapy, Surface antigen, Surface Antigen Antibody.

HBV Reverse Seroconversion and Intervention with Vaccination after Allogeneic HSCT in Patients with Previous HBV Infection.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2921-2921
Author(s):  
Masahiro Onozawa ◽  
Hiroe Kanamori ◽  
Kaoru Kahata ◽  
Takeshi Kondo ◽  
Shuichi Ota ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Carrier Status ◽  
Serological Markers ◽  
Hematopoietic Stem ◽  
Hbv Vaccine

Abstract Appearance of anti-hepatitis B surface antigen antibody (anti-HBs) and clearance of hepatitis B virus (HBV) from serum usually indicates resolution of hepatitis in patients infected with HBV. However, in most patients in whom HBV has been eliminated from serum, HBV DNA is still detectable in the liver using polymerase chain reaction. Reactivation of this dormant HBV in the liver is known as reverse seroconversion (RS). Previously, we reported that HBV-RS after allogeneic hematopoietic stem cell transplantation (allo-HSCT) was a frequent late-onset complication that can be predicted by careful monitoring of progressive disappearance of anti-HBs. RS hepatitis after allo-HSCT is thought to be a phenomenon caused by naive donor immunity after loss of recipient-oriented immunity against HBV. We speculated that vaccination could prevent reactivation of HBV in allo-HSCT recipients. Safety and efficacy of recombinant HBV vaccine in allo-HSCT recipients have already been confirmed. We studied HBV serological markers in 23 patients with anti-HBs and/or anti-HBc before allo-HSCT who were followed for more than 1 year. Patients’ characters are following; Age at HSCT 22 to 65 (median, 38) years; M:F ratio 14:9; Hematological disorders CML 6, AML 1, ALL 4, MDS 4, SAA 2, NHL 4, MM 1 and CAEBV 1; Serological markers anti-HBc(+) and anti-HBs(+) 17, anti-HBc(+) and anti-HBs(−) 3, anti-HBc(−) and anti-HBs(+) 3. No patients had a prior history of vaccination or HBV-specific immunoglobulin usage. All patients were negative for hepatitis B surface antigen (HBsAg) and were considered to have previous HBV infection. The follow-up period varied from 12 to 116 (median, 36) months. Eighteen patients were followed without intervention. Five patients were vaccinated with recombinant HBV vaccine by the standard three-dose protocol after cessation of immunosuppressant administration. RS was defined as disappearance of anti-HBs and appearance of HBsAg and HBV-DNA with or without clinical hepatitis. Progressive decreases in anti-HBs titer were observed in all pre-HSCT anti-HBs-positive recipients. In 18 of the 20 patients with pre-HSCT anti-HBs, anti-HBs titer decreased to less than the protective value (<10.0 mIU/ml) at 4 to 38 (median, 12) months after HSCT. RS occurred in 11 of the 18 patients without intervention (61%) at 12 to 51 (median, 20) months after HSCT. Timing of onset and severity of hepatitis were not correlated. In patients with RS, anti-HBs had disappeared 0 to 26 (median, 8) months prior to RS. Although re-seroconversion occurred in 7 patients, the remaining 4 patients remained in HBV carrier status after resolution of transient hepatitis. In the 7 patients with re-seroconversion, hepatitis subsided and HBsAg disappeared with acquisition of anti-HBe shortly after RS. On the other hand, reappearance of anti-HBs was delayed by 6 to 51 (median, 32) months after onset of RS. Four of the 5 patients with intervention were successfully immunized by HBV vaccine (80%), and none of those 5 patients had RS during the follow-up period (12–40 months; median, 20 months). In conclusion, all patients with previous HBV infection are considered to be at high risk for RS after allo-HSCT. Recombinant HBV vaccines effectively immunize allo-HSCT recipients and are protective against HBV-RS.

scholarly journals Hepatitis B surface antigen-negative, but HBV DNA-positive patients in Bangladesh

2013 ◽  
Vol 38 (3) ◽  
pp. 104-107 ◽  
Author(s):  
MA Mahtab ◽  
SMF Akbar ◽  
S Rahman
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Healthy Subjects ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Hbv Infection ◽  
B Virus ◽  
Control And Management

Hepatitis B surface antigen (HBsAg) is regarded as sole marker of hepatitis B virus (HBV) infection in Bangladesh and most other developing countries. However, some HBV-negative subjects may harbor HBV DNA and transfusion of their blood may cause HBV infection in recipients. HBV DNA was checked in 20 patients with cryptogenic liver cirrhosis, 10 patients with hepatocellular carcinoma without any known etiology, and 10 apparently healthy subjects with elevated levels of serum alanine aminotransferase (ALT). HBV DNA was detected in 8 of 20 patients with cryptogenic liver cirrhosis, 1 of 10 patients with hepatocellular carcinoma, and 2 of 10 apparent healthy subjects with elevated ALT. However, all of them were negative for HBsAg in the sera. This study indicates that some additional mechanisms should be developed for detection of HBsAg-negative HBV-infected subjects for efficient control and management of HBV infection in Bangladesh. DOI: http://dx.doi.org/10.3329/bmrcb.v38i3.14337 Bangladesh Med Res Counc Bull 2012; 38(3): 104-107 (December)

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