scholarly journals Molecular Detection of Trypanosoma spp. in Questing and Feeding Ticks (Ixodidae) Collected from an Endemic Region of South-West Australia

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1037
Author(s):  
Anna-Sheree Krige ◽  
R. C. Andrew Thompson ◽  
Anke Seidlitz ◽  
Sarah Keatley ◽  
Julia Wayne ◽  
...  
Keyword(s):  
High Resolution ◽  
Molecular Detection ◽  
Current Knowledge ◽  
Population Decline ◽  
Molecular Screening ◽  
Endemic Region ◽  
High Resolution Melt ◽  
South West ◽  
Questing Ticks ◽  
Mode Of Transmission

A growing number of indigenous trypanosomes have been reported to naturally infect a variety of Australian wildlife with some species of Trypanosoma implicated in the population decline of critically endangered marsupials. However, the mode of transmission of Australian trypanosomes is unknown since their vectors remain unidentified. Here we aimed to fill this current knowledge gap about the occurrence and identity of indigenous trypanosomes in Australian invertebrates by conducting molecular screening for the presence of Trypanosoma spp. in native ticks collected from south-west Australia. A total of 231 ticks (148 collected from vegetation and 83 retrieved directly from 76 marsupial hosts) were screened for Trypanosoma using a High-Resolution Melt (HRM) qPCR assay. An overall Trypanosoma qPCR positivity of 37% (46/125) and 34% (26/76) was detected in questing ticks and host-collected (i.e., feeding) ticks, respectively. Of these, sequencing revealed 28% (35/125) of questing and 28% (21/76) of feeding ticks were infected with one or more of the five species of trypanosome previously reported in this region (T. copemani, T. noyesi, T. vegrandis, T. gilletti, Trypanosoma sp. ANU2). This work has confirmed that Australian ticks are capable of harbouring several species of indigenous trypanosome and likely serve as their vectors.

Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences

2020 ◽  
Vol 236 ◽  
pp. 111260 ◽  
Author(s):  
Mohsen Arbabi ◽  
Hossein Hooshyar ◽  
Majid Lotfinia ◽  
Mohamad Ali Bakhshi
Keyword(s):  
High Resolution ◽  
Molecular Detection ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Rdna Sequences

Nucleosome dynamics

2006 ◽  
Vol 73 ◽  
pp. 109-119 ◽  
Author(s):  
Chris Stockdale ◽  
Michael Bruno ◽  
Helder Ferreira ◽  
Elisa Garcia-Wilson ◽  
Nicola Wiechens ◽  
...  
Keyword(s):  
High Resolution ◽  
Chromatin Structure ◽  
Current Knowledge ◽  
Dynamic Properties ◽  
Structure And Dynamics ◽  
Nucleosome Dynamics ◽  
Sharp Focus ◽  
Recent Advances ◽  
Insight Into ◽  
Chromatin Structure And Dynamics

In the 30 years since the discovery of the nucleosome, our picture of it has come into sharp focus. The recent high-resolution structures have provided a wealth of insight into the function of the nucleosome, but they are inherently static. Our current knowledge of how nucleosomes can be reconfigured dynamically is at a much earlier stage. Here, recent advances in the understanding of chromatin structure and dynamics are highlighted. The ways in which different modes of nucleosome reconfiguration are likely to influence each other are discussed, and some of the factors likely to regulate the dynamic properties of nucleosomes are considered.

scholarly journals Rapid Determination of Lymphogranuloma Venereum Serovars of Chlamydia trachomatis by Quantitative High-Resolution Melt Analysis (HRMA)

2012 ◽  
Vol 50 (11) ◽  
pp. 3751-3753 ◽  
Author(s):  
J. Twin ◽  
M. P. Stevens ◽  
S. M. Garland ◽  
A. M. Zaia ◽  
S. N. Tabrizi
Keyword(s):  
High Resolution ◽  
Chlamydia Trachomatis ◽  
Rapid Determination ◽  
Lymphogranuloma Venereum ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

2013 ◽  
Vol 14 (8) ◽  
pp. 913-922 ◽  
Author(s):  
Greyson P Twist ◽  
Roger Gaedigk ◽  
J Steven Leeder ◽  
Andrea Gaedigk
Keyword(s):  
High Resolution ◽  
Homologous Gene ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Sequence Variations

A rapid method for sex identification in Cannabis sativa using High Resolution Melt (HRM) analysis.

Botany ◽  
2021 ◽  
Author(s):  
Erin Jacqueline Gilchrist ◽  
Daniela Hegebarth ◽  
Shumin Wang ◽  
Teagen D. Quilichini ◽  
Jason Sawler ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Method ◽  
X Chromosome ◽  
Cannabis Sativa ◽  
Sex Identification ◽  
Male Plant ◽  
High Resolution Melt ◽  
Male And Female ◽  
High Resolution Melt Analysis ◽  
Plant Sex

We report the identification of two SNPs in Cannabis sativa that are associated with female and male plant sex phenotypes, and are located on the top arm of the X chromosome. High Resolution Melt analysis was used to develop and validate a novel, rapid method for sex identification in medical/recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy, and can also be used to differentiate between male and female Humulus lupulus (hop) plants.

A novel pentaplex real time (RT)- PCR high resolution melt curve assay for simultaneous detection of emetic and enterotoxin producing Bacillus cereus in food

Food Control ◽  
2016 ◽  
Vol 60 ◽  
pp. 560-568 ◽  
Author(s):  
Fereidoun Forghani ◽  
Prashant Singh ◽  
Kun-Ho Seo ◽  
Deog-Hwan Oh
Keyword(s):  
High Resolution ◽  
Bacillus Cereus ◽  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
High Resolution Melt
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