scholarly journals Detection of Anti-Echinococcus granulosus Antibodies in Humans: An Update from Pakistan

Pathogens ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 29
Author(s):  
Huma Khan ◽  
Haroon Ahmed ◽  
Muhammad Sohail Afzal ◽  
Usman Ayub Awan ◽  
Muhammad Khurram ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Blood Analysis ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Medical Centers ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test ◽  
Near Future ◽  
Human Cystic Echinococcosis ◽  
Echinococcus Granulosus Sensu Lato

Human cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of Echinococcus granulosus sensu lato that causes economic losses by affecting livestock and also poses a public health threat worldwide. The present study is the first retrospective report on the seroprevalence of anti-E. granulosus antibodies in humans in Pakistan. The study used data from 93 blood analysis reports of patients suspected of having CE from different medical centers in Lahore, Pakistan. Out of 93 sera samples, 20 (21.5%) were seropositive, and higher seropositivity (17.2%) was recorded with the indirect hemagglutination test (IHA) than with enzyme-linked immunosorbent assay (ELISA). The findings indicated that age, gender, and year had no significant relationship with the seropositivity of CE. The current study provides directions towards the management of the disease in the near future in Pakistan.

scholarly journals Echinococcus granulosus sensu lato and Taenia hydatigena in pig in southern Brazil

2015 ◽  
Vol 24 (2) ◽  
pp. 227-229 ◽  
Author(s):  
Danieli Urach Monteiro ◽  
Sônia de Avila Botton ◽  
Alexandre Alberto Tonin ◽  
Karen Luisa Haag ◽  
Germano Musskopf ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Rio Grande ◽  
Northern Region ◽  
Economic Losses ◽  
Meat Industry ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Taenia Hydatigena ◽  
Cysticercus Tenuicollis ◽  
Echinococcus Granulosus Sensu Lato

This study aimed to identify the parasitical etiologic agents of visceral cysts in pigs from the central/northern region of Rio Grande do Sul State, Brazil. Fifty-eight cysts were found in livers during veterinary inspection of swine slaughtered from January 2008 to 2012. Collected samples were submitted to macroscopic and molecular analyzes. Polymerase chain reaction (PCR), DNA sequencing and BLAST alignment of sequences was used to molecular characterization of the samples. By PCR 10.3% (6/58) of tested samples were positive for Echinococcus granulosus sensu lato and 56.9% (33/58) for Cysticercus tenuicollis. In this study, it was verified the occurrence of larval forms of E. granulosus sensu lato and Taenia hydatigena in pig herds from the central/northern region of Rio Grande do Sul State. The presence of both parasites is relevant due to the economic losses for the meat industry. Additionally, E. granulosus sensulato has zoonotic importance and may be infecting pig herds in southern Brazil.

scholarly journals Serological diagnosis of persistent infection with Anaplasma marginale bacteria in cattle

2015 ◽  
Vol 39 (1) ◽  
pp. 33-39
Author(s):  
Hasanain AJ. Al-gharban
Keyword(s):  
Hematological Parameters ◽  
Acute Infection ◽  
Hypochromic Anemia ◽  
Anaplasma Marginale ◽  
Blood Analysis ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Cattle ◽  
Persistently Infected ◽  
Significant Difference

Bovine anaplasmosis is one of the tick-borne diseases caused by Anaplasma marginale bacteria which can cause high economic losses to livestock. Cattle that recovered from acute infection become carriers without clinical signs related to the disease and these bacteria can persist for lifetime in the blood. The present study was conducted to detect antibodies of persistently infected cattle with A. marginale in Wasit province/ Iraq. A total of 100 blood samples were collected randomly from cattle over one year old. Blood smears were prepared, stained with Giemsa,s stain and subjected to microscopic examination for detection of Anaplasma marginale bacteria within an infected RBCs, while serum samples were tested by a competitive enzyme - linked immunosorbent assay test (cELISA) for detection of antibodies in persistently infected cases . Mythic 18 Vet system was used as blood analysis for blood parameters measurement. Results of blood smear examination revealed 13 acute cases, while ELISA detected 35% of carriers. Whereas, the hematological parameters showed that the acutely infected cattle had the macrocytic hypochromic anemia, the persistently infected cattle displaed the microcytic hypochromic and the normocytic hypochromic anemia. Results of blood analysis revealed a significant difference (P˂0.05) in hematological parameters of acute and chronic cases. It has been concluded that cELISA is a reliable screening test for detection of antibodies specific to Anaplasma marginale bacteria.

scholarly journals Development of an improved anesthesia protocol to increase CF1 mice survival in a portal vein infection with Echinococcus granulosus sensu lato protoscoleces

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06496
Author(s):  
Nathalia P. Scioscia ◽  
Patricia E. Pensel ◽  
Guillermo M. Denegri ◽  
María Celina Elissondo
Keyword(s):  
Portal Vein ◽  
Echinococcus Granulosus ◽  
Echinococcus Granulosus Sensu Lato
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