scholarly journals Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 192 ◽  
Author(s):  
Sanae Akkaoui ◽  
Anders Johansson ◽  
Maâmar Yagoubi ◽  
Dorte Haubek ◽  
Adnane El hamidi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Periodontal Diseases ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Psidium Guajava ◽  
Minimal Bactericidal Concentration ◽  
Origanum Vulgare ◽  
Neutralizing Capacity ◽  
Broth Microdilution Method

In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.

scholarly journals Interference of heating on the antimicrobial activity and chemical composition of Origanum vulgare L. (Lamiaceae) essential oil

2008 ◽  
Vol 28 (2) ◽  
pp. 418-422 ◽  
Author(s):  
Evandro Leite de Souza ◽  
Tânia Lúcia Montenegro Stamford ◽  
Edeltrudes de Oliveira Lima ◽  
José Maria Barbosa Filho ◽  
Márcia Ortiz Mayo Marques
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Origanum Vulgare

scholarly journals Chemical composition and antimicrobial activity of Thymus pannonicus All. (Lamiaceae) essential oil

2008 ◽  
Vol 3 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Zoran Maksimović ◽  
Marina Milenković ◽  
Dragana Vučićević ◽  
Mihailo Ristić
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Maximum Activity ◽  
Flowering Plant ◽  
Moderate Activity ◽  
Microdilution Method ◽  
Agar Disc ◽  
Broth Microdilution Method

AbstractThis paper presents the results of a study on chemical composition and antimicrobial activity of Thymus pannonicus All. (Lamiaceae) essential oil from Vojvodina province (north of Serbia). The investigated oil was hydrodistilled from a flowering plant and analysed by GC and GC-MS. Fifty-three constituents were identified (>97% of total oil), with geranial (41.42%, w/w) and neral (29.61%, w/w) as the most prominent. The antimicrobial activity of the oil was evaluated using agar disc diffusion and broth microdilution method against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, two strains of Klebsiella pneumoniae and two strains of Candida albicans. The essential oil exhibited antimicrobial activity to varying degrees against all tested strains. The maximum activity of T. Pannonicus oil was observed against E. coli, S. aureus and both tested strains of C. Albicans (MIC = 50 µ/ml, each). Moderate activity was observed against P. aeruginosa and one of the tested strains of K. Pneumoniae (MIC = 200 µ/ml), while E. faecalis and the other strain of K. Pneumoniae expressed a higher degree of resistance (MIC > 200 µ/ml). This study confirms that essential oil of T. pannonicus possesses remarkable in vitro antimicrobial activity against several medicinally important pathogens. This is attributable to lemon-scented citral, a mixture of geranial and neral, which has well-documented antimicrobial activity against a range of bacteria and fungi.

scholarly journals Chemical composition and antimicrobial activity of fruit essential oils of Myrica gale, a neglected non-wood forest product

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Kristina Ložienė ◽  
Juozas Labokas ◽  
Vaida Vaičiulytė ◽  
Jurgita Švedienė ◽  
Vita Raudonienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Chiral Phase ◽  
Myrica Gale ◽  
Broth Microdilution Method ◽  
Fruit Samples

The study aimed to establish the chemical composition of fruit essential oils of M. gale and test their activities against the selected pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii), yeasts (Candida albicans, C. parapsilosis), fungi (Aspergillus fumigatus, A. flavus) and dermatophytes (Trichophyton rubrum, T. mentagrophytes). Fruit samples from natural (Western Lithuania) and anthropogenic (Eastern Lithuania) M. gale populations were studied separately. Essential oils were isolated from dried fruits by hydrodistillation and analysed by GC/FID and GC/MS methods; enantiomeric composition of α-pinene was established by chiral-phase capillary GC. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) of essential oils were determined using the broth microdilution method. Plants from the natural population with a humid marine climate accumulated significantly higher amounts of fruit essential oils (3.34±0.05%) than those from the anthropogenic population with a more continental climate (2.71±0.22%). In total, 39 volatiles including α-pinene (23.52–27.17%), 1,8-cineole (17.19–18.84%) and α-phellandrene (9.47–10.03%) as main compounds were identified. Chiral analysis demonstrated that (1S)-(–)-α-pinene prevailed over (1R)-(+)-α-pinene and amounted to 94.09–95.28% of all fraction of this monoterpene. The antimicrobial study in vitro indicated that C. parapsilosis, dermatophytes and Aspergillus fungi were more susceptible to fruit essential oils of M. gale, whereas E. coli and C. albicans were weakly inhibited even at the highest essential oil concentration. The strongest growth-inhibitory and bactericidal effect of sweet gale essential oil was established on S. aureus. This could be attributed to the major essential oil compounds with known antimicrobial activity, such as α-pinene, 1,8-cineole and a-phellandrene. Keywords: Myrica gale; essential oil; chemical compounds; terpenes; enantiomers; antimicrobial.

Chemical composition and antimicrobial activity of the essential oil of senescent leaves of guava (Psidium guajava L.)

2019 ◽  
pp. 1-5 ◽  
Author(s):  
Trishna Chaturvedi ◽  
Swati Singh ◽  
Indrajeet Nishad ◽  
Ajay Kumar ◽  
Neha Tiwari ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Psidium Guajava

scholarly journals Chemical composition and antimicrobial activity of essential oils extracted from Amomum muricarpum Elmer from North Vietnam

2022 ◽  
Vol 11 (4) ◽  
pp. 523-530
Author(s):  
D. B. Thin ◽  
V. Q. Thanh ◽  
B. B. Thinh
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
The Philippines ◽  
Volatile Components ◽  
Broth Microdilution Method ◽  
North Vietnam

Abstract. Recent years have seen the development of bacterial resistance to currently available antibiotics, which necessitates a search for new antimicrobial agents. Amomum muricarpum Elmer is a widely used medicinal plant species in the genus Amomum (family Zingiberaceae) that is commonly found in Laos, the Philippines, China, and Vietnam. The present article describes the chemical composition and antimicrobial activity of essential oils extracted from the leaves and rhizomes of A. muricarpum from North Vietnam. The hydrodistilled essential oil was analyzed using gas chromatography and gas chromatography-mass spectrometry, with the broth microdilution method designed to evaluate its antimicrobial efficacy. The absolute yield of essential oils amounted to 0.11% and 0.13% (v/w) for leaves and rhizomes, respectively, on a dry weight basis. It was found that the leaves and rhizomes of A. muricarpum produce oils abounding in monoterpenes. Of the total identified volatile components in the leaf oil (97.18%), three main constituents include α-pinene (40.45%), linalool (12.34%), and β-pinene (10.31%). In the rhizome oil, the main constituents include α-pinene (48.10%), β-pinene (20.32%), and linalool (7.56%) of the total identified volatile components (98.08%). An antimicrobial activity test indicates that essential oils from the leaves and rhizome of A. muricarpum inhibit the growth of Staphylococcus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) of 200 µg/ml. In addition, the rhizome essential oil also exhibits antimicrobial activity against Bacillus cereus ATCC 14579, with a MIC value of 200 µg/ml. The results indicate the potential of essential oils extracted from A. muricarpum as a source of antimicrobial agents.

scholarly journals ANTIMICROBIAL ACTIVITY OF ESSENTIAL OILS OF Origanum vulgare AND Ocimum basilicum AGAINST Vibrio parahaemolyticus AND Vibrio vulnificus AND ADDITION OF THESE OILS ON Mugil platanus FILLETS

2021 ◽  
Vol 46 (4) ◽  
pp. 1-6
Author(s):  
Greyce Silveira MELLO ◽  
Juliana Fernandes ROSA ◽  
Débora Rodrigues da SILVEIRA ◽  
Patrícia Gomes VIVIAN ◽  
Natacha Deboni CERESER ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Food Poisoning ◽  
Ocimum Basilicum ◽  
Minimal Bactericidal Concentration ◽  
Origanum Vulgare ◽  
Vibrio Genus

Species of the Vibrio genus are commonly reported as agents of food poisoning outbreaks associated with fish consumption. The objective of this study was to assess the antimicrobial activity of the essential oils of Origanum vulgare and Ocimum basilicum against Vibrio parahaemolyticus and Vibrio vulnificus, as well as to assess their addition to fillets of Mugil platanus and sensorial acceptance among consumers. The antimicrobial activity was analyzed by the disc diffusion test and minimal bactericidal concentration (MBC). M. platanus fillets, experimentally contaminated, were marinated for 24 hours in a solution containing 1.0% and 1.5% of essential oil of O. vulgare. For the sensory analysis, samples marinated in 1.5% of O. vulgare oil were prepared and offered to 100 testers. The oil of O. basilicum shows no antimicrobial activity, so its application would be ineffective; for such reason the subsequent tests were not performed. However, the essential oil of O. vulgare produced inhibition halo diameters ranging from 24.6 to 34.1 mm, on average, and the MBC ranging from 3.9% to 15.6%. When added to the product, the essential oil of O. vulgare eliminated the microorganisms that were experimentally inoculated. In the sensorial analysis, samples marinated in 1.5% of O. vulgare essential oil obtained an average of 6.82 of acceptance note among consumers.

scholarly journals Variability in chemical composition and antimicrobial activity of essential oil of Rosa × damascena Herrm. from mountainous regions of Iran

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Mansureh Ghavam ◽  
Afsaneh Afzali ◽  
Maria Manconi ◽  
Gianluigi Bacchetta ◽  
Maria Letizia Manca
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Environmental Conditions ◽  
Raw Materials ◽  
Rosa Damascena ◽  
Mountainous Regions ◽  
Aspergillus Brasiliensis ◽  
Suitable Area

Abstract Background Essential oil of Rosa × damascena Herrm. is one of the most valuable and important raw materials for the flavor and fragrance industry. The cultivation of this plant has ancient origins, and Kashan was one of the first mountainous regions of Iran dealing with the cultivation of R. × damascena. In this study, both chemical composition and antimicrobial activity of different rose essential oils obtained from five mountainous areas of Kashan region (Maragh, Qamsar, Sadeh, Javinan, and Kamoo) has been investigated along with the influence of the environmental conditions on these properties. Results Results showed that yield and chemical composition of essential oils obtained from Rosa × damascena were significantly affected by the collection area. In particular, the yield of oils varied from ~0.08 to ~0.132% and citronellol (36.70-9.18%), geraniol (12.82-0.47%), nonadecane (22.73-10.36%), heneicosane (31.7-11.43%), and 1-nonadecene (6.03-3.93%) have been detected as main compounds in all the plants collected, but at different concentrations depending on the collection area. The best fragrance and the highest yield were found in the oil from Kamoo area. Similarly to the chemical composition, the antimicrobial activity of the essential oils was affected by their origin, and essential oil obtained from plants collected from Kamoo area disclosed the highest antibacterial and antifungal efficacy. Its inhibition halos were 17.33±0.58 mm against Aspergillus brasiliensis, 15.67±0.58 mm against Staphylococcus aureus, and 12.33±0. 58 mm against Streptococcus pyogenes. Essential oils of R. damascena were also effective against Gram-negative Pseudomonas aeruginosa and they had a MIC value of 62.50 μg/mL irrespective of the collection area (except the oil from Javinan area). On the contrary, the highest antifungal power against Candida albicans yeast was reached using the essential oil obtained from plants collected in Javinan region (MIC and MBC ~62.50 μg/mL). Conclusions Overall results underline the influence of environmental conditions of the different areas of Kashan region, on the chemical composition of and antimicrobial activity of the essential oils of Rosa × damascena. In addition, results disclosed that Kamoo seemed to be the most suitable area for the competitive cultivation of R. × damascena to the intensive production of aromatic flower oil and natural antimicrobial essential oils.

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