scholarly journals Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2396
Author(s):  
Yu-Jeng Chen ◽  
Hsuan-Chun Lai ◽  
Chung-Cheng Lin ◽  
Zhuan Yi Neoh ◽  
Wen-Shi Tsai
Keyword(s):  
Host Range ◽  
Virus Detection ◽  
Bottle Gourd ◽  
Bitter Gourd ◽  
New Delhi ◽  
Viral Dna ◽  
Pathogenic Viruses ◽  
Viral Components ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of Squash leaf curl China virus (SLCCNV) and Tomato leaf curl New Delhi virus (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops

scholarly journals Scanometric Detection of Tomato Leaf Curl New Delhi Viral DNA Using Mono- and Bifunctional AuNP-Conjugated Oligonucleotide Probes

ACS Omega ◽  
2019 ◽  
Vol 4 (6) ◽  
pp. 10094-10107 ◽  
Author(s):  
Gunasekaran Dharanivasan ◽  
Denison Michael Immanuel Jesse ◽  
Thangavelu Rajamuthuramalingam ◽  
Ganapathy Rajendran ◽  
Sathappan Shanthi ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
Oligonucleotide Probes ◽  
Viral Dna ◽  
Tomato Leaf Curl ◽  
Leaf Curl

scholarly journals Resistance Against Melon Chlorotic Mosaic Virus and Tomato Leaf Curl New Delhi Virus in Melon

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2913-2919 ◽  
Author(s):  
Gustavo Romay ◽  
Michel Pitrat ◽  
Herve Lecoq ◽  
Catherine Wipf-Scheibel ◽  
Pauline Millot ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Dominant Gene ◽  
Virus Detection ◽  
Recessive Gene ◽  
Tomato Leaf ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Thirty-one melon accessions were screened for resistance to the begomoviruses Melon chlorotic mosaic virus (MeCMV) and Tomato leaf curl New Delhi virus (ToLCNDV). Five accessions presented nearly complete resistance to both viruses. Accession IC-274014, showing the highest level of resistance to both viruses, was crossed with the susceptible cultivar Védrantais. The F1, F2, F3/F4, and both backcross progenies were mechanically inoculated with MeCMV. Plants without symptoms or virus detection by enzyme-linked immunosorbent assay and/or PCR were considered as resistant. The segregations were compatible with two recessive and one dominant independent genes simultaneously required for resistance. Inheritance of resistance to ToLCNDV in the F2 was best explained by one recessive gene and two independent dominant genes simultaneously required. Some F3 and F4 families selected for resistance to MeCMV also were resistant to ToLCNDV, suggesting that common or tightly linked genes were involved in resistance to both viruses. We propose the names begomovirus resistance-1 and Begomovirus resistance-2 for these genes (symbols bgm-1 and Bgm-2). Resistance to MeCMV in IC-274014 was controlled by bgm-1, Bgm-2, and the recessive gene melon chlorotic mosaic virus resistance (mecmv); resistance to ToLCNDV was controlled by bgm-1, Bgm-2, and the dominant gene Tomato leaf curl New Delhi virus resistance (Tolcndv).

scholarly journals LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 466
Author(s):  
Ahmed Mahas ◽  
Norhan Hassan ◽  
Rashid Aman ◽  
Tin Marsic ◽  
Qiaochu Wang ◽  
...  
Keyword(s):  
Low Cost ◽  
Virus Detection ◽  
High Sensitivity ◽  
Plant Viruses ◽  
Economic Losses ◽  
New Delhi ◽  
Dna Viruses ◽  
Point Of Use ◽  
Tomato Leaf Curl ◽  
Leaf Curl

One important factor for successful disease management is the ability to rapidly and accurately identify the causal agent. Plant viruses cause severe economic losses and pose a serious threat to sustainable agriculture. Therefore, optimization of the speed, sensitivity, feasibility, portability, and accuracy of virus detection is urgently needed. Here, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR–Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes. Our assay detected TYLCV and ToLCNDV in infected plants with high sensitivity and specificity. Our newly developed assay can be performed in ~1 h and provides easy-to-interpret visual readouts using a simple, low-cost fluorescence visualizer, making it suitable for point-of-use applications.

Potato apical leaf curl disease: current status and perspectives on a disease caused by tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Current Status ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl
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