scholarly journals Effects of Conventional Flotation Frothers on the Population of Mesophilic Microorganisms in Different Cultures

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 653 ◽  
Author(s):  
Mohammad Jafari ◽  
Mehdi Golzadeh ◽  
Sied Shafaei ◽  
Hadi Abdollahi ◽  
Mahdi Gharabaghi ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Acidithiobacillus Ferrooxidans ◽  
Lower Sensitivity ◽  
Acidithiobacillus Thiooxidans ◽  
Methyl Isobutyl ◽  
Flotation Process ◽  
Leptospirillum Ferrooxidans ◽  
Pine Oil ◽  
Pure Cultures ◽  
Extraction Of Metals

Bioleaching is an environment-friendly and low-investment process for the extraction of metals from flotation concentrate. Surfactants such as collectors and frothers are widely used in the flotation process. These chemical reagents may have inhibitory effects on the activity of microorganisms through a bioleaching process; however, there is no report indicating influences of reagents on the activity of microorganisms in the mixed culture which is mostly used in the industry. In this investigation, influences of typical flotation frothers (methyl isobutyl carbinol and pine oil) in different concentrations (0.01, 0.10, and 1.00 g/L) were examined on activates of bacteria in the mesophilic mixed culture (Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans, and Acidithiobacillus thiooxidans). For comparison purposes, experiments were repeated by pure cultures of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans in the same conditions. Results indicated that increasing the dosage of frothers has a negative correlation with bacteria activities while the mixed culture showed a lower sensitivity to the toxicity of these frothers in comparison with examined pure cultures. Outcomes showed the toxicity of Pine oil is lower than methyl isobutyl carbinol (MIBC). These results can be used for designing flotation separation procedures and to produce cleaner products for bio extraction of metals.

Experimental Research on Coal Biodesulfurization by Mixed Culture Column Leaching

2012 ◽  
Vol 512-515 ◽  
pp. 2500-2504 ◽  
Author(s):  
Yu Yang ◽  
Gai Mei Ren ◽  
Xiang Wang ◽  
Li Yang
Keyword(s):  
Mixed Culture ◽  
Acidithiobacillus Thiooxidans ◽  
Total Sulfur ◽  
Desulfurization Rate ◽  
Column Leaching ◽  
Leptospirillum Ferrooxidans ◽  
Coal Desulfurization ◽  
Inner Diameter ◽  
Lower Ratio ◽  
Sulfur In Coal

The removal of sulfur in coal before its combustion can reduce its environmental pollution, and microbial desulfurization should have good application prospects. Some species of bacteria have been used in the process of coal desulfurization, and our previous study demonstrated that mixed culture should be more efficient than pure culture by shaking cultivation. In this study, column leaching was investigated, due to its similarity to the industrial heap leaching. The mixed culture consisted of three strains of bacteria, which were Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans respectively. The ratio of the three strains of bacteria was 1: 1: 1. The column (50 cm height, 650 mm Inner Diameter) bioleaching experiments were carried out at ambient temperature. After desulfurization for 18 days, the ultimate desulfurization rate was 28.66% for total sulfur, which was higher than the previous study of J.Cara (with total sulfur desulfurization rate for 24% after 125 days). This study showed that ferric ions should be in proper concentration, and Leptospirillum ferrooxidans might be in lower ratio for reducing the precipitation of jarosite and improving the efficient of coal desulfurization.

scholarly journals Use of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans in the Recovery of Heavy Metals from Landfill Leachates

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3336
Author(s):  
Tomasz Kamizela ◽  
Anna Grobelak ◽  
Malgorzata Worwag
Keyword(s):  
Heavy Metals ◽  
Mixed Culture ◽  
Reverse Osmosis ◽  
Landfill Leachate ◽  
Acidithiobacillus Ferrooxidans ◽  
High Efficiency ◽  
Metal Removal ◽  
Acidithiobacillus Thiooxidans ◽  
Landfill Leachates ◽  
Removal Of Heavy Metals

Among the methods used to remove metals and their compounds from landfill leachates with low application costs and high efficiency are bioleaching and biosorption. The most effective bacteria used in the metal removal process are Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The aim of the study was to determine the usefulness of the A. ferrooxidans and A. thiooxidans population in removing heavy metals from landfill leachate. In addition, development opportunities for bacterial population using landfill leachate as growth medium were identified. The substrate for the research was the raw leachate before the reverse osmosis process. In order to increase the efficiency of trace elements removal and recovery from leachate, variable combinations have been used which differ by the addition of sulfuric acid, A. ferrooxidans culture, A. thiooxidans culture, mixed culture containing populations of both bacteria, and elemental sulfur. Based on the research, it was found that the removal of heavy metals from leachate was a selective process. High bioleaching efficiency, from 80% to 90%, was obtained for all metals for which the sample acidification or sulfur addition was used. The simultaneous combination of both these additives turned out to be the most advantageous. The A. thiooxidans culture was the most effective in bioleaching reverse osmosis effluents. For the A. ferrooxidans culture used, much lower efficiencies were obtained, while by contrast, the use of mixed culture of two bacterium species had no significant effect.

scholarly journals Adaptation of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans strains on sphalerite concentrate from mining waste

Respuestas ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 72-83
Author(s):  
Lina M. Miranda Arroyave ◽  
Marco Antonio Márquez Godoy ◽  
Luz Marina Ocampo Carmona
Keyword(s):  
Energy Source ◽  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Sulphate ◽  
Mining Waste ◽  
Acidithiobacillus Thiooxidans ◽  
Secondary Products ◽  
External Energy ◽  
Leptospirillum Ferrooxidans ◽  
External Energy Source ◽  
High Concentrations

One of the main characteristics of the microorganisms used in the leaching process is their capacity to adapt to aggressive environments, characterized by a notable presence of heavy metals. In this study the adaptation of the strains Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans was evaluated on a sphalerite concentrate from mining waste. In the adaptation tests, the energy source (ferrous sulphate) was gradually replaced by percentages of mineral pulp, ending with subcultures without the addition of an external energy source. The results show that the strains A. ferrooxidans and A. thiooxidans are more resistant to high concentrations of sphalerite, compared to the strain of L. ferrooxidans, since, in the case of this strain, it was necessary to repeat some tests (8% of pulp), since a deficient development was evident. This was associated with factors such as the decrease of the Fe+2 energy source, the increase of the pulp density, the accumulation of toxic metals and secondary products of the dissolution of minerals and the increase of the pH.

Analysis of Sulfur Metasecretome in Mixed Cultures of Acidithiobacillus Thiooxidans and Acidithiobacillus Ferrooxidans

2009 ◽  
Vol 71-73 ◽  
pp. 151-154
Author(s):  
R.A. Bobadilla Fazzini ◽  
Pilar Parada Valdecantos
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
De Novo ◽  
Hypothetical Protein ◽  
Acidithiobacillus Thiooxidans ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Cytochrome C Biogenesis ◽  
Sulfur Bacteria ◽  
Pure Cultures

The role of biomolecules in bioleaching of copper sulphide minerals carried out by bacterial consortia with predominating acidithiobacilli species is of outmost interest. The proteomic analysis on bioleaching bacterial strains have been focused up to date on full Acidithiobacillus ferrooxidans proteome, allowing the identification of proteins belonging to the general stress response, phosphate limiting conditions and the ones linked to the periplasmic fraction. Our study shows for the first time the differential expression of secreted proteins by means of standard proteomics between pure cultures of Acidithiobacillus thiooxidans and in mixture with A. ferroxidans (metasecretome) grown in sulfur, where a set of proteins is de novo synthesized in the mixed culture, identifying an Omp40-like protein possibly related to bacterial adhesion, an hypothetical protein PSEEN2944 with unknown function and up-regulation of a cytochrome c biogenesis protein, findings that give an insight into the role of proteins at the sulfur – bacteria interface, highlighting the outputs of bacterial interactions in biomining environments at the protein secretion level.

Oxidation of Chalcopyrite (CuFeS2) by Acidithiobacillus Ferrooxidans and a Mixed Culture of Acidithiobacillus Ferrooxidans and Acidithiobacillus Thiooxidans Like Bacterium in Shake Flasks

2009 ◽  
Vol 71-73 ◽  
pp. 385-388 ◽  
Author(s):  
E.R. Mejía ◽  
J.D. Ospina ◽  
M.A. Márquez ◽  
A.L. Morales
Keyword(s):  
Redox Potential ◽  
Mixed Culture ◽  
Acidithiobacillus Ferrooxidans ◽  
Mineral Content ◽  
Ferrous Sulphate ◽  
Acidithiobacillus Thiooxidans ◽  
Mineral Particle ◽  
Ph Values ◽  
Shake Flasks ◽  
The Fourier Transform

Chalcopyrite bioleaching process using Acidithiobacillus ferrooxidans and a mixed culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans like bacterium was carried out. Two mineral particle sizes were evaluated, 200 and 325 Tyler mesh. The strains were adapted by gradually decreasing of the main energy sources and increasing in the mineral content. The experiments were performed in absence of ferrous sulphate and elemental sulfur. When the mixed culture was used, pH values were always over 2,1, indicating a probable passivation of Acidithiobacillus thiooxidans in the consortium. For both cultures, the Cu2+ dissolution occurred at relatively low redox potential values, around 400mV–450mV, while at high redox potential values, 550mV, chalcopyrite dissolution was inhibited. Copper lixiviation was around 40% for both tests. The Fourier Transform-Infrared spectra showed that the main oxidation phase is jarosite. The results showed that chalcopyrite oxidation is more dependent on the redox potential than particle size or type of culture used.

Microbial Leaching of Copper from Waste Electronic Scraps

2012 ◽  
Vol 508 ◽  
pp. 228-232
Author(s):  
Jin Qiu Xu ◽  
Jian Feng Bai ◽  
Jing Wei Wang ◽  
Bo Liang ◽  
He Cheng ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Acidithiobacillus Ferrooxidans ◽  
Pure Culture ◽  
Raw Materials ◽  
Experimental Results ◽  
Acidithiobacillus Thiooxidans ◽  
Conductive Coating ◽  
Sieve Fraction ◽  
Microbial Leaching ◽  
Leaching Efficiency

Microbial leaching was used to mobilize copper from two kinds of waste electronic scraps used as raw materials for printed wire boards. Both of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans used to solubilize copper from the samples. The experimental results demonstrated that the percentages of copper basically increased with decrease of sieve fraction of the samples. The green non-conductive coating of the waste electronic scraps was proved to have little effect on the leaching efficiency of both of the pure culture of Acidithiobacillus ferrooxidans and Acidithiobacil thiooxidans or their mixed culture. Acidithiobacillus ferrooxidans has larger solubilizing capability for copper than Acidithiobacillus thiooxidans.

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