scholarly journals Assessment of the Toxicity of Natural Oils from Mentha piperita, Pinus roxburghii, and Rosa spp. Against Three Stored Product Insects

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 861 ◽  
Author(s):  
Marwa I. Mackled ◽  
Mervat EL-Hefny ◽  
May Bin-Jumah ◽  
Trandil F. Wahba ◽  
Ahmed A. Allam
Keyword(s):  
Diphenyl Ether ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica ◽  
Gas Chromatography Mass Spectrometry ◽  
Mentha Piperita ◽  
Natural Material ◽  
Chemical Agent ◽  
Pinus Roxburghii ◽  
Stored Product Insects ◽  
Natural Oils

Three natural oils extracted from Mentha piperita, Pinus roxburghii, and Rosa spp. were assessed in order to determine their insecticidal activity against the adults of three stored product insects: the rice weevil (Sitophilus oryzae L.), the lesser grain borer (Rhyzopertha dominica, Fabricius), and the red flour beetle (Tribolium castaneum, Herbst.). By Gas chromatography–mass spectrometry (GC/MS) analysis, the main compounds in the n-hexane oil from Rosa spp. were determined to be methyl eugenol (52.17%), phenylethyl alcohol (29.92%), diphenyl ether (7.75%), and geraniol (5.72%); in the essential oil from M. piperita, they were menthone (20.18%), 1,8-cineole (15.48%), menthyl acetate (13.13%), caryophyllene (4.82%), β-pinene (4.37%), and D-limonene (2.81%); and from the foliage of P. roxburghii, they were longifolene (19.52%), caryophyllene (9.45%), Δ-3-carene (7.01%), α-terpineol (6.75%), and γ-elemene (3.88%). S. oryzae and R. dominica were reared using sterilized wheat grains, and T. castaneum was reared on wheat flour mixed with yeast (10:1, w/w), all under laboratory conditions (27 ± 1 °C and 65% ± 5% Relative humidity (R.H). Two toxicity bioassays were used, as well as contact using thin film residues and fumigation bioassays. The results indicated that M. piperita caused a high toxicity for S. oryzae compared to other insects. High significant variations were observed between the tested M. piperita doses against the stored insects, and this natural material could be used to control insects that infect the grains. Also, the data indicated that the Rosa spp. oil had a low-toxicity effect against these insects compared to other oils. We recommend using natural oils against the stored weevils and petals, rather than the chemical agent, so as to serve human health.

scholarly journals Natural Formulation Based on Diatomaceous Earth and Botanicals against Stored Product Insects

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 613 ◽  
Author(s):  
Ivan Paponja ◽  
Vlatka Rozman ◽  
Anita Liška
Keyword(s):  
Diatomaceous Earth ◽  
Corn Oil ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica ◽  
Lower Amount ◽  
Favorable Effect ◽  
Stored Product Insects ◽  
N Form ◽  
Negative Side Effects ◽  
Natural Formulation

Diatomaceous earth (DE) has long been known as a potential protectant for stored cereals against various stored product insects. Despite favorable effect for the environment and human health, DE has some negative side effects on the treated commodity. In order to minimize negative response and to improve its efficacy, this paper represents a study of developed natural formulation based on DE SilicoSec® enhanced with botanicals (essential oil lavender, corn oil, and bay leaves dust) and silica gel. The activity of formulation (labeled as N Form) was tested against Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Tribolium castaneum (Herbst) in seed wheat and barley under controlled conditions. As a reference comparative value, DE SilicoSec® was used. N Form showed higher efficacy than DE, especially in barley at the lowest concentration, inducing higher mortality of all three insect species. The highest average progeny inhibition was recorded in R. dominica population both in seed wheat and barley with 94.9% and 96.3% of inhibition, respectively, followed with S. oryzae and T. castaneum inhibition of 90.6% and 86.1%, respectively, in wheat and 94.9% and 89.7%, respectively, in barley. Results indicate that the developed natural formulation N Form enhanced the activity of DE SilicoSec® using lower amount of DE dust and that it could be successfully implemented for storage of cereals as alternatives to chemical pesticides for stored product insect control.

Effects of red versus white wheat bran on rate of growth and feeding of some stored-product beetles

2000 ◽  
Vol 80 (3) ◽  
pp. 661-663
Author(s):  
Noel D. G. White ◽  
Colin J. Demianyk ◽  
Paul G. Fields
Keyword(s):  
Spring Wheat ◽  
Seed Coat ◽  
Wheat Bran ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica ◽  
Hard Red Spring Wheat ◽  
White Wheat ◽  
Stored Product Insects ◽  
Wood Boring ◽  
Insect Attack

The red pigment in the seed coat of hard red spring wheat is produced by phenolics. Most of the wheats grown in western Canada are hard red spring varieties; however cultivars with a whiter seed coat are being developed for their better milling and baking attributes. Because phenolics serve to protect some plants against insect attack, we compared the susceptibility of white and red spring wheat to attack by stored-product insects. Tenebrio molitor (Tenebrionidae) larvae reared on red wheat bran gained less weight than larvae reared on white wheat bran but this insect has a long developmental period and does not attack sound grain. Tribolium castaneum (Tenebrionidae) pupal weights were not affected by the type of wheat milled products on which the larvae fed. A feeding bioassay showed that red bran did not act as an antifeedant for T. molitor, T. castaneum, or Sitophilus oryzae (Curculionidae). However, it acted as a feeding stimulant for Rhyzopertha dominica (Bostrichidae), which is related to wood-boring insects. It is unlikely that white wheat in storage would be more prone to insect damage than red wheat. Key words: Bran, colour, wheat, phenolics, stored-product insects

scholarly journals An influence of herbs on the sex structure in populations of insect pests of stored products

2012 ◽  
Vol 52 (4) ◽  
pp. 463-466
Author(s):  
Małgorzata Kłyś
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Rice Weevil ◽  
Insect Species ◽  
Rhyzopertha Dominica ◽  
Mentha Piperita ◽  
Lesser Grain Borer ◽  
Sex Structure ◽  
Males And Females ◽  
Lavandula Officinalis

Abstract The effects of powdered herbs (lavender Lavandula officinalis L., peppermint Mentha piperita L. and basil Ocimium basilicum L.) added to the food substances of three insect species: the rice weevil Sitophilus oryzae (L.), lesser grain borer Rhyzopertha dominica (F.), and the saw-toothed grain beetle Oryzaephilus surinamensis (L.) on the life span and development of the three insect species as well as habitat conditions, sex structure, and the survivability of males and females were considered. The experiments were conducted in the laboratory at a temperature of 28°C and relative humidity (RH) 60 ±5%. In all the studied populations, high female mortality was found (♂/♀ < 1). The studied populations were: the rice weevil and the lesser grain borer cultured in wheat grain with herb additions, and the saw-toothed grain beetle cultured in semolina with herb additions. Among the herbs used, peppermint caused a significant effect in the form of increased mortality rates among females of S. oryzae and O. surinamensis. Lavender and basil had a similar impact among R. dominica females. The herbs applied in the experiments intensified the mortality of females and, consequently, led to the disappearance of entire populations.

scholarly journals A Pilot Study of Natural Formulation Activity in the Protection of Stored Wheat and Barley Against the Stored-Product Insects

Poljoprivreda ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 43-49
Author(s):  
Ivan Paponja ◽  
Vlatka Rozman ◽  
Pavo Lucić ◽  
Anita Liška
Keyword(s):  
Silica Gel ◽  
Corn Oil ◽  
Sitophilus Oryzae ◽  
Control Measures ◽  
Insect Species ◽  
Rhyzopertha Dominica ◽  
Initial Population ◽  
Complete Suppression ◽  
Stored Product Insects ◽  
Natural Formulation

The stored-product insects are one of the major causes of losses in the stored cereals. Most of control measures still rely on a synthetic pesticide usage, but due to its negative side effects on the goods, human health, and the environment, there is an urgent need for an alternative control. A natural formulation based on the diatomaceous earth (DE) SilicoSec®, enhanced with the botanicals (essential oil lavender, corn oil, and bay leaves dust) and the silica gel was developed. The aim of the study was to test the activity of the developed formulation as a postharvest protectant of seed wheat and barley in the suppression Sitophilus oryzae (L.), Rhyzopertha dominica (F.) and Tribolium castaneum (Herbst). As a reference comparative value, the DE SilicoSec® was applied. Subsequent to the six months of storage under the simulated warehouse conditions, the formulation has completely suppressed the initial population development of all three tested insect species, both in wheat and barley. In wheat, a complete suppression was detected at the dose of 500 ppm against T. castaneum and 600 ppm against both R. dominica and S. oryzae. In barley, a complete suppression was detected at the doses of 500 ppm, 400 ppm, and 600 ppm against R. dominica, T. castaneum and S. oryzae, respectively. Conclusively, the results of this study indicate that the developed natural formulation based on the DE, botanicals, and silica gel was highly effective against the three major stored‐product insect species, providing a long-term safe storage of wheat and barley seeds.

scholarly journals Determinação de concentrações de Beauveria bassiana (Bals.) Vuill. para o controle de insetos-pragas de grãos armazenados

1997 ◽  
Vol 26 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Alcides Moino Jr. ◽  
Sérgio B. Alves
Keyword(s):  
Beauveria Bassiana ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica

Determinou-se a suscetibilidade de Sitophilus oryzae (L.), S. zeamais (Motsch.) e Rhyzopertha dominica (Fabr.) a dois isolados do fungo Beauveria bassiana (Bals.) Vuill. Para isso foram usados recipientes contendo arroz beneficiado, inoculado previamente com conídios dos isolados, infestados com as três espécies de insetos. Esses recipientes foram mantidos a 26 ± 0,5°C, 70 ± 10% de UR e fotofase de 12 horas. As três espécies foram suscetíveis aos dois isolados do patógeno. Os dois isolados foram eficientes para o controle de S. oryzae, na concentração de 0,5 g de conídios/100 g de grãos. Para S. zeamais, o isolado 604 foi mais eficiente na concentração de 0,1 g de conídios/100 g de grãos. Com relação a R. dominica, foram suficientes dosagens de 0,01 g (isolado 476) e 0,05 g (isolado 604)/100 g de grãos, confirmando a maior suscetibilidade desta espécie ao patógeno.

scholarly journals Mobility of Stored Product Beetles after Exposure to a Combination Insecticide Containing Deltamethrin, Methoprene, and a Piperonyl Butoxide Synergist Depends on Species, Concentration, and Exposure Time

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 151 ◽  
Author(s):  
Frank H. Arthur ◽  
Christos G. Athanassiou ◽  
W. Robert Morrison
Keyword(s):  
Exposure Time ◽  
General Pattern ◽  
Sitophilus Oryzae ◽  
Piperonyl Butoxide ◽  
Rhyzopertha Dominica ◽  
Cryptolestes Ferrugineus ◽  
Curve Fit ◽  
Exposure Interval ◽  
Grain Protectant ◽  
The Relationship

Adults of Rhyzopertha dominica (F.), the lesser grain borer, Cryptolestes ferrugineus (Stephens), the rusty grain beetle, and Sitophilus oryzae (L.), the rice weevil, were exposed for 1, 24, and 72 h on wheat treated with concentrations of 0% (untreated controls) to 100% of the proposed label rate of an experimental formulation of deltamethrin + Methoprene + piperonyl butoxide synergist. Movement and velocity of movement were assessed after each exposure time using a camera-based monitoring system (Ethovision®). Movement of R. dominica decreased with increasing concentration and exposure time, so that movement had virtually ceased at the 48 and 72 h exposures. Cryptolestes ferrugineus was less susceptible compared to R. dominica, but there was still a general pattern of decreased movement and velocity of movement with increasing concentration and exposure time. Sitophilus oryzae was the least susceptible species, with less differences at the 1 h exposure interval compared to the other two species, but after 24–72 h, the patterns of declining movement and velocity were apparent as the concentration increased. Data were analyzed using curve-fit equations to show the relationship between concentration and exposure time for each species. Results show that the Ethovison system can be used to assess the sub-lethal effects of exposure to grain protectant insecticides and elucidate behavioral variation between different stored product insects.

scholarly journals Preliminary Study on the Differences in Hydrocarbons Between Phosphine-Susceptible and -Resistant Strains of Rhyzopertha dominica (Fabricius) and Tribolium castaneum (Herbst) Using Direct Immersion Solid-Phase Microextraction Coupled with GC-MS

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1565
Author(s):  
Ihab Alnajim ◽  
Manjree Agarwal ◽  
Tao Liu ◽  
Beibei Li ◽  
Xin Du ◽  
...  
Keyword(s):  
Cuticular Hydrocarbons ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Rhyzopertha Dominica ◽  
Gas Chromatography Mass Spectrometry ◽  
Stored Grain ◽  
Phosphine Resistance ◽  
Significant Difference ◽  
Resistant Strains ◽  
Direct Immersion

Phosphine resistance is a worldwide issue threatening the grain industry. The cuticles of insects are covered with a layer of lipids, which protect insect bodies from the harmful effects of pesticides. The main components of the cuticular lipids are hydrocarbon compounds. In this research, phosphine-resistant and -susceptible strains of two main stored-grain insects, T. castaneum and R. dominica, were tested to determine the possible role of their cuticular hydrocarbons in phosphine resistance. Direct immersion solid-phase microextraction followed by gas chromatography-mass spectrometry (GC-MS) was applied to extract and analyze the cuticular hydrocarbons. The results showed significant differences between the resistant and susceptible strains regarding the cuticular hydrocarbons that were investigated. The resistant insects of both species contained higher amounts than the susceptible insects for the majority of the hydrocarbons, sixteen from cuticular extraction and nineteen from the homogenized body extraction for T. castaneum and eighteen from cuticular extraction and twenty-one from the homogenized body extraction for R. dominica. 3-methylnonacosane and 2-methylheptacosane had the highest significant difference between the susceptible and resistant strains of T. castaneum from the cuticle and the homogenized body, respectively. Unknown5 from the cuticle and 3-methylhentriacontane from the homogenized body recorded the highest significant differences in R. dominica. The higher hydrocarbon content is a key factor in eliminating phosphine from entering resistant insect bodies, acting as a barrier between insects and the surrounding phosphine environment.

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