scholarly journals A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

Sensors ◽  
2016 ◽  
Vol 16 (11) ◽  
pp. 1900 ◽  
Author(s):  
Christoph Slouka ◽  
David Wurm ◽  
Georg Brunauer ◽  
Andreas Welzl-Wachter ◽  
Oliver Spadiut ◽  
...  
Chemosensors ◽  
2017 ◽  
Vol 5 (3) ◽  
pp. 24 ◽  
Author(s):  
Christoph Slouka ◽  
Georg Brunauer ◽  
Julian Kopp ◽  
Michael Strahammer ◽  
Jens Fricke ◽  
...  

Chemosensors ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 27
Author(s):  
Georg Christoph Brunauer ◽  
Oliver Spadiut ◽  
Alfred Gruber ◽  
Christoph Slouka

Electrochemical impedance spectroscopy is a powerful tool in life science for cell and pathogen detection, as well as for cell counting. The measurement principles and techniques using impedance spectroscopy are highly diverse. Differences can be found in used frequency range (β or α regime), analyzed quantities, like charge transfer resistance, dielectric permittivity of double layer capacitance and in off- or online usage. In recent contributions, applications of low-frequency impedance spectroscopy in the α regime were tested for determination of cell counts and metabolic burden in Escherichia coli and Saccharomyces cerevisiae. The established easy to use methods showed reasonable potential in the lab scale, especially for S. cerevisiae. However, until now, measurements for cell counts in food science are generally based on Thoma cell counting chambers. These microscopic cell counting methods decelerate an easy and quick prediction of yeast viability, as they are labor intensive and result in a time delayed response signal. In this contribution we tested our developed method using low frequency impedance spectroscopy locally at an industrial brewery propagation site and compared results to classic cell counting procedures.


2012 ◽  
Vol 455-456 ◽  
pp. 760-764
Author(s):  
Wei Bo Huang ◽  
Jing Zhang ◽  
Xin Mao Li ◽  
Ping Lu

- Electrochemical impedance spectroscopy (EIS) was used to study the thickness and curing temperature influence on the aging behavior of polyaspartic ester (PAE) polyurea under salt fog exposure in this paper. The result has demonstrated that coating thickness and curing temperature had great influence on aging behavior of the PAE-t-HDI prepolymer-D2000H65 aliphatic polyurea (T3). The low frequency impedance after 1day exposure increased from 5.31×1010Ω·cm to 7.96×1010Ω while coating thickness was increased from 40μm to 200μm, and the low frequency impedance of 200μm coating was 7.42×1010Ω·cm2 high after 200 days exposure. The low frequency impedance after 1day and 200 days exposure increased by 0.83×1010 Ω·cm2 and 0.39×109Ω·cm2 respectively, while curing temperature were increased from 60°C to 80°C. Suggestions have been made, namely that increase the coating thickness and curing temperature can improve low frequency impedance of PAE polyurea, as a result, improve the protective properties of the coatings.


2020 ◽  
Vol MA2020-02 (20) ◽  
pp. 1570-1570
Author(s):  
Maurilio Pereira Gomes ◽  
Isolda Costa ◽  
Nadine Pebere ◽  
Jesualdo Luis Rossi ◽  
Mireille Turmine ◽  
...  

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