scholarly journals Time Sequential Single-Cell Patterning with High Efficiency and High Density

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3672 ◽  
Author(s):  
Yang Liu ◽  
Dahai Ren ◽  
Xixin Ling ◽  
Weibin Liang ◽  
Jing Li ◽  
...  
Keyword(s):  
Single Cell ◽  
High Efficiency ◽  
Single Cells ◽  
Capture Efficiency ◽  
Jurkat Cells ◽  
Objective Lens ◽  
Cell Capture ◽  
Cell Trapping ◽  
Loop Channel ◽  
Single Cell Trapping

Single-cell capture plays an important role in single-cell manipulation and analysis. This paper presents a microfluidic device for deterministic single-cell trapping based on the hydrodynamic trapping mechanism. The device is composed of an S-shaped loop channel and thousands of aligned trap units. This arrayed structure enables each row of the device to be treated equally and independently, as it has row periodicity. A theoretical model was established and a simulation was conducted to optimize the key geometric parameters, and the performance was evaluated by conducting experiments on MCF-7 and Jurkat cells. The results showed improvements in single-cell trapping ability, including loading efficiency, capture speed, and the density of the patterned cells. The optimized device can achieve a capture efficiency of up to 100% and single-cell capture efficiency of up to 95%. This device offers 200 trap units in an area of 1 mm2, which enables 100 single cells to be observed simultaneously using a microscope with a 20× objective lens. One thousand cells can be trapped sequentially within 2 min; this is faster than the values obtained with previously reported devices. Furthermore, the cells can also be recovered by reversely infusing solutions. The structure can be easily extended to a large scale, and a patterned array with 32,000 trap sites was accomplished on a single chip. This device can be a powerful tool for high-throughput single-cell analysis, cell heterogeneity investigation, and drug screening.

scholarly journals Efficient analysis of a small number of cancer cells at the single-cell level using an electroactive double-well array

Lab on a Chip ◽  
2016 ◽  
Vol 16 (13) ◽  
pp. 2440-2449 ◽  
Author(s):  
Soo Hyeon Kim ◽  
Teruo Fujii
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Single Cells ◽  
Capture Efficiency ◽  
Single Cell Level ◽  
Cell Capture ◽  
Cell Level ◽  
Cell Trapping ◽  
Highly Efficient ◽  
Single Cell Trapping

The electroactive double well-array consists of trap-wells for highly efficient single-cell trapping using dielectrophoresis (cell capture efficiency of 96 ± 3%) and reaction-wells that confine cell lysates for analysis of intracellular materials from single cells.

scholarly journals A Microfluidic Array Device for Single Cell Capture and Intracellular Ca2+ Response Analysis Induced by Dynamic Biochemical Stimulus

2021 ◽  
Author(s):  
Wenbo Wei ◽  
Miao Zhang ◽  
Zhongyuan Xu ◽  
Weifeng Li ◽  
Lixin Cheng ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
K562 Cells ◽  
Cellular Heterogeneity ◽  
Response Analysis ◽  
Cell Capture ◽  
Cell Trapping ◽  
Dynamic Stimulus ◽  
Single Cell Trapping ◽  
Varying Environments

A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca2+ signalling in single cells by applying extracellular dynamic biochemical stimulus. This microfluidic array consists of multiple radially aligned flow channels with equal intersection angles, which was designed by a combination of stagnation point flow and physical barrier. Numerical simulation results and trajectory analysis shown the effectiveness of this single cell trapping device. Fluorescent experiment results demonstrated the effects of flow rate and frequency of dynamic stimulus on the profiles of biochemical concentration which exposed on captured cells. In this array chip, the captured single cells in each trapping channels were able to receive identical extracellular dynamic biochemical stimuli which being transmitted from the entrance at the middle of the microfluidic array. Besides, after loading dynamic Adenosine Triphosphate (ATP) stimulation on captured cells by this device, consistent average intracellular Ca2+ dynamics phase and cellular heterogeneity were observed in captured single K562 cells. Furthermore, this device is able to be used for investigating cellular respond in single cells to temporally varying environments by modulating the stimulation signal in terms of concentration, pattern, and duration of exposure.

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

scholarly journals A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4077-4088 ◽  
Author(s):  
Lindong Weng ◽  
Felix Ellett ◽  
Jon Edd ◽  
Keith H. K. Wong ◽  
Korkut Uygun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Permeability ◽  
Single Cells ◽  
Cell Membrane Permeability ◽  
Volumetric Change ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Passive Pumping

A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.

Single Cell Stimulation Assay: Microfluidic Substance Delivery to a Laterally Trapped Cell

2011 ◽  
Author(s):  
Kyohei Terao ◽  
Murat Gel ◽  
Atsuhito Okonogi ◽  
Takaaki Suzuki ◽  
Fumikazu Oohira ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Side Wall ◽  
Cell Stimulation ◽  
Cell Trapping ◽  
Cell Responses ◽  
Single Cell Trapping ◽  
Intracellular Response ◽  
Chemical Stimuli ◽  
Simple Flow

We propose a novel cell stimulation device for the analysis of cell responses to chemical stimuli. In order to deliver chemical substances to target single cells, we developed a microfluidic device having microchannels and apertures in the side wall to subject stimuli to laterally trapped cells. The channels were designed to allow simple flow control with single syringe pump. We demonstrated single cell trapping and culturing of pancreatic β cell with the device. To test its feasibility in cell stimulation assay, intracellular response of the cell to glucose stimulation was demonstrated.

Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1370-1377 ◽  
Author(s):  
Cheng-Kun He ◽  
Ya-Wen Chen ◽  
Ssu-Han Wang ◽  
Chia-Hsien Hsu
Keyword(s):  
Single Cell ◽  
Biomedical Research ◽  
Microfluidic Chip ◽  
High Efficiency ◽  
Single Cells ◽  
Cellular Heterogeneity ◽  
Cell Capture

A new microfluidics technique for high-efficiency paring and analyzing multiple single cells can facilitate cellular heterogeneity studies important for biological and biomedical research.

scholarly journals A microfluidic device enabling high-efficiency single cell trapping

2015 ◽  
Vol 9 (1) ◽  
pp. 014101 ◽  
Author(s):  
D. Jin ◽  
B. Deng ◽  
J. X. Li ◽  
W. Cai ◽  
L. Tu ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Efficiency ◽  
Cell Trapping ◽  
Single Cell Trapping

scholarly journals Dual Dean entrainment with volume ratio modulation for efficient co-encapsulation: Extreme single-cell indexing

2021 ◽  
Author(s):  
Jack Harrington ◽  
Luis Blay Esteban ◽  
Jonathan Butement ◽  
Andres F. Vallejo ◽  
Simon I. R. Lane ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Volume Ratio ◽  
Cell Suspensions ◽  
Capture Efficiency ◽  
Cell Capture ◽  
Optimal Conditions ◽  
Signal To Noise ◽  
Cell Diversity ◽  
Larger Sample

AbstractThe future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ~1 million cells/hour, while increasing assay signal-to-noise with cell multiplet rates of ~2.5% and capturing ~70% of cells. The method, called Pirouette-seq, extends our capacity to investigate biological systems.TOC AbstractPirouette-seq involves cell and reporter bead inertial ordering for efficient co-encapsulation, achieving a throughput of 1 million cells/hour, a 2.5% multiplet rate and a 70% cell capture efficiency.

scholarly journals Pipette Petri Dish Single-Cell Trapping (PP-SCT) in Microfluidic Platforms: A Passive Hydrodynamic Technique

Fluids ◽  
2018 ◽  
Vol 3 (3) ◽  
pp. 51 ◽  
Author(s):  
Vigneswaran Narayanamurthy ◽  
Tze Lee ◽  
Al’aina Khan ◽  
Fahmi Samsuri ◽  
Khairudin Mohamed ◽  
...  
Keyword(s):  
Single Cell ◽  
Flow Rate ◽  
Single Cell Analysis ◽  
Petri Dish ◽  
Human Lung Cancer ◽  
Cell Analysis ◽  
Straight Channel ◽  
Cell Capture ◽  
Cell Trapping ◽  
Single Cell Trapping

Microfluidics-based biochips play a vital role in single-cell research applications. Handling and positioning of single cells at the microscale level are an essential need for various applications, including genomics, proteomics, secretomics, and lysis-analysis. In this article, the pipette Petri dish single-cell trapping (PP-SCT) technique is demonstrated. PP-SCT is a simple and cost-effective technique with ease of implementation for single cell analysis applications. In this paper a wide operation at different fluid flow rates of the novel PP-SCT technique is demonstrated. The effects of the microfluidic channel shape (straight, branched, and serpent) on the efficiency of single-cell trapping are studied. This article exhibited passive microfluidic-based biochips capable of vertical cell trapping with the hexagonally-positioned array of microwells. Microwells were 35 μm in diameter, a size sufficient to allow the attachment of captured cells for short-term study. Single-cell capture (SCC) capabilities of the microfluidic-biochips were found to be improving from the straight channel, branched channel, and serpent channel, accordingly. Multiple cell capture (MCC) was on the order of decreasing from the straight channel, branch channel, and serpent channel. Among the three designs investigated, the serpent channel biochip offers high SCC percentage with reduced MCC and NC (no capture) percentage. SCC was around 52%, 42%, and 35% for the serpent, branched, and straight channel biochips, respectively, for the tilt angle, θ values were between 10–15°. Human lung cancer cells (A549) were used for characterization. Using the PP-SCT technique, flow rate variations can be precisely achieved with a flow velocity range of 0.25–4 m/s (fluid channel of 2 mm width and 100 µm height). The upper dish (UD) can be used for low flow rate applications and the lower dish (LD) for high flow rate applications. Passive single-cell analysis applications will be facilitated using this method.

scholarly journals A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 412
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Taeyeon Kim ◽  
Jihyun Jung ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Biology ◽  
Single Cells ◽  
Data Sampling ◽  
Measurement Sensitivity ◽  
Accurate Analysis ◽  
High Throughput Analysis ◽  
Cell Trapping ◽  
Chip Technology ◽  
Single Cell Trapping

Electrical impedance biosensors combined with microfluidic devices can be used to analyze fundamental biological processes for high-throughput analysis at the single-cell scale. These specialized analytical tools can determine the effectiveness and toxicity of drugs with high sensitivity and demonstrate biological functions on a single-cell scale. Because the various parameters of the cells can be measured depending on methods of single-cell trapping, technological development ultimately determine the efficiency and performance of the sensors. Identifying the latest trends in single-cell trapping technologies afford opportunities such as new structural design and combination with other technologies. This will lead to more advanced applications towards improving measurement sensitivity to the desired target. In this review, we examined the basic principles of impedance sensors and their applications in various biological fields. In the next step, we introduced the latest trend of microfluidic chip technology for trapping single cells and summarized the important findings on the characteristics of single cells in impedance biosensor systems that successfully trapped single cells. This is expected to be used as a leading technology in cell biology, pathology, and pharmacological fields, promoting the further understanding of complex functions and mechanisms within individual cells with numerous data sampling and accurate analysis capabilities.

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