scholarly journals Super-Resolution Reconstruction of Cell Pseudo-Color Image Based on Raman Technology

Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4076
Author(s):  
Yang ◽  
Zhu ◽  
Wang ◽  
Yang ◽  
Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectrum ◽  
Background Noise ◽  
Color Image ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Pseudo Color

Raman spectroscopy visualization is a challenging task due to the interference of complex background noise and the number of selected measurement points. In this paper, a super-resolution image reconstruction algorithm for Raman spectroscopy is studied to convert raw Raman data into pseudo-color super-resolution imaging. Firstly, the Raman spectrum data of a single measurement point is measured multiple times to calculate the mean value to remove the random background noise, and innovatively introduce the Retinex algorithm and the median filtering algorithm which improve the signal-to-noise ratio. The novel method of using deep neural network performs a super-resolution reconstruction operation on the gray image. An adaptive guided filter that automatically adjusts the filter radius and penalty factor is proposed to highlight the contour of the cell, and the super-resolution reconstruction of the pseudo-color image of the Raman spectrum is realized. The average signal-to-noise ratio of the reconstructed pseudo-color image sub-band reaches 14.29 db, and the average value of information entropy reaches 4.30 db. The results show that the Raman-based cell pseudo-color image super-resolution reconstruction algorithm is an effective tool to effectively remove noise and high-resolution visualization. The contrast experiments show that the pseudo-color image Kullback–Leiber (KL) entropy of the color image obtained by the method is small, the boundary is obvious, and the noise is small, which provide technical support for the development of sophisticated single-cell imaging Raman spectroscopy instruments.

Model-based reconstruction algorithm in the detection of acute trauma-related lesions in brain CT examinations

2021 ◽  
pp. 197140092110087
Author(s):  
Andrea De Vito ◽  
Cesare Maino ◽  
Sophie Lombardi ◽  
Maria Ragusi ◽  
Cammillo Talei Franzesi ◽  
...  
Keyword(s):  
Image Quality ◽  
Iterative Reconstruction ◽  
Signal To Noise Ratio ◽  
Reconstruction Algorithm ◽  
Signal To Noise ◽  
Subjective Image Quality ◽  
Model Based ◽  
Hybrid Iterative Reconstruction ◽  
Noise Ratio ◽  
Enhanced Computed Tomography

Background and purpose To evaluate the added value of a model-based reconstruction algorithm in the assessment of acute traumatic brain lesions in emergency non-enhanced computed tomography, in comparison with a standard hybrid iterative reconstruction approach. Materials and methods We retrospectively evaluated a total of 350 patients who underwent a 256-row non-enhanced computed tomography scan at the emergency department for brain trauma. Images were reconstructed both with hybrid and model-based iterative algorithm. Two radiologists, blinded to clinical data, recorded the presence, nature, number, and location of acute findings. Subjective image quality was performed using a 4-point scale. Objective image quality was determined by computing the signal-to-noise ratio and contrast-to-noise ratio. The agreement between the two readers was evaluated using k-statistics. Results A subjective image quality analysis using model-based iterative reconstruction gave a higher detection rate of acute trauma-related lesions in comparison to hybrid iterative reconstruction (extradural haematomas 116 vs. 68, subdural haemorrhages 162 vs. 98, subarachnoid haemorrhages 118 vs. 78, parenchymal haemorrhages 94 vs. 64, contusive lesions 36 vs. 28, diffuse axonal injuries 75 vs. 31; all P<0.001). Inter-observer agreement was moderate to excellent in evaluating all injuries (extradural haematomas k=0.79, subdural haemorrhages k=0.82, subarachnoid haemorrhages k=0.91, parenchymal haemorrhages k=0.98, contusive lesions k=0.88, diffuse axonal injuries k=0.70). Quantitatively, the mean standard deviation of the thalamus on model-based iterative reconstruction images was lower in comparison to hybrid iterative one (2.12 ± 0.92 vsa 3.52 ± 1.10; P=0.030) while the contrast-to-noise ratio and signal-to-noise ratio were significantly higher (contrast-to-noise ratio 3.06 ± 0.55 vs. 1.55 ± 0.68, signal-to-noise ratio 14.51 ± 1.78 vs. 8.62 ± 1.88; P<0.0001). Median subjective image quality values for model-based iterative reconstruction were significantly higher ( P=0.003). Conclusion Model-based iterative reconstruction, offering a higher image quality at a thinner slice, allowed the identification of a higher number of acute traumatic lesions than hybrid iterative reconstruction, with a significant reduction of noise.

Speech in noise testing before and after grommet insertion

2012 ◽  
Vol 126 (10) ◽  
pp. 1010-1015 ◽  
Author(s):  
V Possamai ◽  
G Kirk ◽  
A Scott ◽  
D Skinner
Keyword(s):  
Small Group ◽  
Background Noise ◽  
Signal To Noise Ratio ◽  
Sound Field ◽  
Noise Signal ◽  
Signal To Noise ◽  
Speech In Noise ◽  
Before And After ◽  
Noise Test ◽  
Noise Ratio

AbstractObjectives:To assess the feasibility of designing and implementing a speech in noise test in children before and after grommet insertion, and to analyse the results of such a test in a small group of children.Methods:Twelve children aged six to nine years who were scheduled to undergo grommet insertion were identified. They underwent speech in noise testing before and after grommet insertion. This testing used Arthur Boothroyd word lists read at 60 dB in four listening conditions presented in a sound field: firstly in quiet conditions, then in signal to noise ratios of +10 (50 dB background noise), 0 (60 dB) and −10 (70 dB).Results:Mean phoneme scores were: in quiet conditions, 28.1 pre- and 30 post-operatively (p = 0.04); in 50 dB background noise (signal to noise ratio +10), 24.2 pre- and 29 post-operatively (p < 0.01); in 60 dB background noise (signal to noise ratio 0), 22.6 pre- and 27.5 post-operatively (p = 0.06); and in 70 dB background noise (signal to noise ratio −10), 13.9 pre- and 21 post-operatively (p = 0.05).Conclusion:This small study suggests that speech in noise testing is feasible in this scenario. Our small group of children demonstrated a significant improvement in speech in noise scores following grommet insertion. This is likely to translate into a significant advantage in the educational environment.

scholarly journals Object Removal and Inpainting Technique by Lattice Boltzmann Method and Exemplar Method in Color Image

2019 ◽  
Vol 8 (6) ◽  
pp. 2008-2011
Keyword(s):  
Lattice Boltzmann Method ◽  
Lattice Boltzmann ◽  
Color Image ◽  
Signal To Noise Ratio ◽  
Similarity Index ◽  
Image Inpainting ◽  
Self Similarity ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Boltzmann Method

In this paper we use image inpainting technique in a color image. Inpainting is a technique in which, a missing area or pixels are replaced by adequate neighbouring pixels so that the resultant image obtained will look as no defect is there. In this paper we have removed an object from the color image and the vacant pixels are filled with the help of the surrounding neighbouring pixels. Two techniques are used here for removing and inpainting an object from the still color image, lattice boltzmann and exemplar method. These techniques were selected as Exemplar method of inpainting was used in many algorithms and have given a good result and lattice Boltzmann was usually used in experiments conducted in fluid dynamics. As motion of pixels can be compared to motion of fluids, lattice boltzmann method gives a better match for filling in the regions. Different variables for the inpainted image was calculated for both the methods. The signal to noise ratio and self-similarity index of the image is calculated for both inpainting techniques. According to the signal to noise ratio and the time taken for inpainting, it is found that lattice Boltzmann method of inpainting gives a better inpainted result..

scholarly journals Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Cerutti ◽  
Morgana D’Amico ◽  
Isotta Cainero ◽  
Gaetano Ivan Dellino ◽  
Mario Faretta ◽  
...  
Keyword(s):  
Autocorrelation Function ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Image Contrast ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Signal To Noise ◽  
Image Correlation Spectroscopy ◽  
Noise Ratio

AbstractQuantifying the imaging performances in an unbiased way is of outmost importance in super-resolution microscopy. Here, we describe an algorithm based on image correlation spectroscopy (ICS) that can be used to assess the quality of super-resolution images. The algorithm is based on the calculation of an autocorrelation function and provides three different parameters: the width of the autocorrelation function, related to the spatial resolution; the brightness, related to the image contrast; the relative noise variance, related to the signal-to-noise ratio of the image. We use this algorithm to evaluate the quality of stimulated emission depletion (STED) images of DNA replication foci in U937 cells acquired under different imaging conditions. Increasing the STED depletion power improves the resolution but may reduce the image contrast. Increasing the number of line averages improves the signal-to-noise ratio but facilitates the onset of photobleaching and subsequent reduction of the image contrast. Finally, we evaluate the performances of two different separation of photons by lifetime tuning (SPLIT) approaches: the method of tunable STED depletion power and the commercially available Leica Tau-STED. We find that SPLIT provides an efficient way to improve the resolution and contrast in STED microscopy.

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