scholarly journals Rapid Analysis for Staphylococcus aureus via Microchip Capillary Electrophoresis

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1334
Author(s):  
Jin Chen ◽  
Yu Sun ◽  
Xiaogai Peng ◽  
Yi Ni ◽  
Fengchao Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Capillary Electrophoresis ◽  
High Speed ◽  
Rapid Determination ◽  
Internal Standard ◽  
Human Beings ◽  
Microchip Capillary Electrophoresis ◽  
A Value ◽  
Pcr Products ◽  
Electrophoresis Microchip

Staphylococcus aureus (S. aureus) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of S. aureus, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of S. aureusnuc gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/μL, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis.

Rapid Determination of Auramine O in Yellow croaker by Microchip Capillary Electrophoresis

2013 ◽  
Vol 30 (4) ◽  
pp. 481
Author(s):  
Haiyun ZHAI ◽  
Jiangmei LI ◽  
Zuanguang CHEN ◽  
Qing ZHOU ◽  
Yufang PAN
Keyword(s):  
Capillary Electrophoresis ◽  
Rapid Determination ◽  
Microchip Capillary Electrophoresis ◽  
Auramine O

Simultaneous Detection of Yersinia Enterocolitica and Listeria Monocytogenes in Foodstuffs by Capillary Electrophoresis and Microchip Capillary Electrophoresis Laser-Induced Fluorescence Detector

2018 ◽  
Vol 101 (6) ◽  
pp. 1833-1838 ◽  
Author(s):  
Yongru Li ◽  
Hongwei Su ◽  
Yajia Lan
Keyword(s):  
Capillary Electrophoresis ◽  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Microchip Capillary Electrophoresis ◽  
Fluorescence Detector ◽  
Sybr Gold ◽  
Pcr Products

Abstract Background: Food safety is one of the most important public health problems in the world, and pathogenic bacterium is a major factor causing serious foodborne diseases. Objective: Two methods of duplex PCR combined with capillary electrophoresis laser-induced fluorescence detector (CE-LIF) and microchip capillary electrophoresis laser-induced fluorescence detector (MCE-LIF) have been developed for the simultaneous detection of Yersinia Enterocolitica and Listeria Monocytogenes in various foods. The specific conservative sequences of these two bacteria were amplified. Methods: After labelled with nucleic acid dye SYBR Gold and SYBR Orange, the PCR products were analyzed by CE-LIF and MCE-LIF, respectively. Under the optimal conditions, the detection of PCR products of the target bacteria was achieved in less than 15 min by CE-LIF and within 6 min by MCE-LIF. Results: The alignment analysis demonstrated that the PCR products had good agreement with the sequences published in GenBank. The CE-LIF method could detect 10 CFU/mL Y. enterocolitica and L. monocytogenes, and the MCE-LIF method could detect 100 CFU/mL Y. enterocolitica and L. monocytogenes. The intraday precisions of migration time and peak area of DNA markers and PCR products were in the range of 1.13 to 1.18% and 1.60 to 6.29%, respectively, for CE-LIF and 1.18 to 1.48% and 2.85 to 4.06%, respectively, for MCE-LIF. Conclusions: The proposed methods could be applied to target bacterial detection infood samples rapidly, sensitively, and specifically. Highlights: Two new methods based on CE and MCE have been developed for the simultaneous detection of Y. enterocolitica and L. monocytogenes in foodstuffs, and they can detect the bacteria directly without any enrichment because of their high sensitivity.

scholarly journals Routine Analysis of Ascorbic Acid in Citrus Juice Using Capillary Electrophoresis

2001 ◽  
Vol 84 (3) ◽  
pp. 987-992 ◽  
Author(s):  
Paul F Cancalon
Keyword(s):  
Ascorbic Acid ◽  
Capillary Electrophoresis ◽  
Vitamin C ◽  
High Speed ◽  
Ethylenediaminetetraacetic Acid ◽  
Internal Standard ◽  
Dehydroascorbic Acid ◽  
Sodium Borate ◽  
Internal Standard Method ◽  
Citrus Juice

Abstract A procedure to monitor citrus juice samples was established to quantitate vitamin C by capillary electrophoresis using a previously developed method. Dilution and filtration were the only preparation requirements and separation was achieved with an uncoated capillary using a 35mM sodium borate buffer (pH 9.3) containing 5% (v/v) acetonitrile at 21 kV and 23°C. Detection was performed by high speed scanning between 200 and 360 nm. From the multiwave length scan, the electropherogram at 270 nm was extracted and used to quantitate ascorbic acid. The ascorbic acid concentration was calculated with an internal standard method, with ferulic acid as internal standard. The level of ascorbic acid during analysis was stabilized with ethylenediaminetetraacetic acid and dithiothreitol was used to reduce dehydroascorbic acid to ascorbic acid to estimate the total vitamin C level. Results were similar to those obtained by liquid chromatography and the method is now used to determine routinely the level of ascorbic acid in citrus juices.

Variability of microchip capillary electrophoresis with conductivity detection

2013 ◽  
Vol 35 (2-3) ◽  
pp. 263-270 ◽  
Author(s):  
Ratna Tantra ◽  
Kenneth Robinson ◽  
Aneta Sikora
Keyword(s):  
Capillary Electrophoresis ◽  
Microchip Capillary Electrophoresis ◽  
Conductivity Detection

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

2018 ◽  
Vol 41 (19) ◽  
pp. 3764-3771 ◽  
Author(s):  
Pornpan Prapatpong ◽  
Nantana Nuchtavorn ◽  
Mirek Macka ◽  
Leena Suntornsuk
Keyword(s):  
Capillary Electrophoresis ◽  
Rapid Determination ◽  
Uv Detection
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