scholarly journals The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 186
Author(s):  
Zili Guo ◽  
Shuting Xiong ◽  
Yuanyuan Xie ◽  
Xianrui Liang
Keyword(s):  
Ellagic Acid ◽  
Ball Mill ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Adsorption Method ◽  
Phyllanthus Urinaria ◽  
Wide Range ◽  
Macroporous Adsorption Resin ◽  
Adsorption Resin

Ellagic acid is a phenolic compound that exhibits both antimutagenic and anticarcinogenic activity in a wide range of assays in vitro and in vivo. It occurs naturally in some foods such as raspberries, strawberries, grapes, and black currants. In this study, a valid and reliable method based on mechanochemical-assisted extraction (MCAE) and macroporous adsorption resin was developed to extract and prepare ellagic acid from Phyllanthus urinaria L. (PUL). The MCAE parameters, acidolysis, and macroporous adsorption resin conditions were investigated. The key MCAE parameters were optimized as follows: the milling time was 5 min, the ball mill speed was 100 rpm, and the ball mill filling rate was 20.9%. Sulfuric acid with a concentration of 0.552 mol/L was applied for the acidolysis with the optimized acidolysis time of 30 min and acidolysis temperature of 40 °C. Additionally, the XDA-8D macroporous resin was chosen for the purification work. Both the static and dynamic adsorption tests were carried out. Under the optimized conditions, the yield of ellagic acid was 10.2 mg/g, and the content was over 97%. This research provided a rapid and efficient method for the preparation of ellagic acid from the cheaply and easily obtained PUL. Meanwhile, it is relatively low-cost work that can provide a technical basis for the comprehensive utilization of PUL.

Synthesis and Characterization of Strong Polar Macroporous Resin Made from Cellulose

2011 ◽  
Vol 346 ◽  
pp. 743-750
Author(s):  
Xin Qi
Keyword(s):  
Ester Group ◽  
Skeletal Structure ◽  
Hydroxyl Group ◽  
Macroporous Resin ◽  
Isothermal Adsorption ◽  
Macroporous Adsorption Resin ◽  
Infrared Spectrometer ◽  
Adsorption Resin ◽  
Different Levels

The macroporous adsorption resin synthesized in this experiment uses cullulose as monomer, adipoyl dichlorid as crosslinker, and cyclohexane as porogenic agent, which three corsslink and polymerize each other, forming the porous skeletal structure. The cellulose processing procedure is as follow: prepare alkali cellulose; crosslink the cellulose (etherification); etherify the cellulose; and functionalize the cellulose. By assaying the perssad characterization of the macroporous resin obtained in this experiment with Fourier infrared spectrometer, we observe hydroxyl group and ester group in the spectrogram. Observing the spectrogram, we find hydroxyl group, indicating that the hydroxyl group in the cellulose has not reacted fully, while the ester group in the spectrogram showing that the ester group has reacted fully with the cellulose. After the aperture characterization for the produced resin with scanning electron microscope (SEM), we find there are unevenly distributed apertures on different levels, which means that new macroporous resin has been synthesized. This paper taking rutin as the adsorbate explores the propertis of static adsorption and desorption of the synthetic macroporous resin, and the influence on its adsorption capability under different situations. The adsorption data shows that the adsorption of rutin on the very resin conforms to the Freundlich isothermal adsorption equation.

Purification of Total Flavonoids from Buddleja officinalis by AB-8 Macroporous Adsorption Resin

2013 ◽  
Vol 641-642 ◽  
pp. 988-992 ◽  
Author(s):  
Ying Wen ◽  
Ya Ting Liu ◽  
Jing Min Zhang ◽  
Lei Guo
Keyword(s):  
Flow Rate ◽  
Ph Value ◽  
Total Flavonoids ◽  
Separation And Purification ◽  
Sample Flow Rate ◽  
Macroporous Adsorption Resin ◽  
Adsorption Resin

The separation and purification of total flavonoids from Buddleja officinalis were carried out by AB-8 macroporous adsorption resin. The optimum adsorption conditions for sample flow rate, pH value and feeding concentration were 2.0 mL/min, 4.5 and 1.6 mg/mL, respectively, and the optimum desorption conditions were obtained by using 2.0 BV of 70% ethanol as desorption solvent at a flow rate of 1.0 mL/min. Under these conditions, the flavonoids content of the final product was 90.43%, increased by 1.7 times than unpurified sample (53.09%).

Evaluation of 99mTc-Label led Vitamin K4 as an Agent for Testicular Imaging

1991 ◽  
Vol 30 (01) ◽  
pp. 35-39 ◽  
Author(s):  
H. S. Durak ◽  
M. Kitapgi ◽  
B. E. Caner ◽  
R. Senekowitsch ◽  
M. T. Ercan
Keyword(s):  
Soft Tissue ◽  
Room Temperature ◽  
Normal Subjects ◽  
Left Testis ◽  
Wide Range ◽  
Activity Ratios ◽  
The Right ◽  
Radioactivity Levels

Vitamin K4 was labelled with 99mTc with an efficiency higher than 97%. The compound was stable up to 24 h at room temperature, and its biodistribution in NMRI mice indicated its in vivo stability. Blood radioactivity levels were high over a wide range. 10% of the injected activity remained in blood after 24 h. Excretion was mostly via kidneys. Only the liver and kidneys concentrated appreciable amounts of radioactivity. Testis/soft tissue ratios were 1.4 and 1.57 at 6 and 24 h, respectively. Testis/blood ratios were lower than 1. In vitro studies with mouse blood indicated that 33.9 ±9.6% of the radioactivity was associated with RBCs; it was washed out almost completely with saline. Protein binding was 28.7 ±6.3% as determined by TCA precipitation. Blood clearance of 99mTc-l<4 in normal subjects showed a slow decrease of radioactivity, reaching a plateau after 16 h at 20% of the injected activity. In scintigraphic images in men the testes could be well visualized. The right/left testis ratio was 1.08 ±0.13. Testis/soft tissue and testis/blood activity ratios were highest at 3 h. These ratios were higher than those obtained with pertechnetate at 20 min post injection.99mTc-l<4 appears to be a promising radiopharmaceutical for the scintigraphic visualization of testes.

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