scholarly journals Effects of Cassava Juice (Manihot esculenta Crantz) on Renal and Hepatic Function and Motor Impairments in Male Rats

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 708
Author(s):  
Eduardo Rivadeneyra-Domínguez ◽  
José Eduardo Pérez-Pérez ◽  
Alma Vázquez-Luna ◽  
Rafael Díaz-Sobac ◽  
Juan Francisco Rodríguez-Landa
Keyword(s):  
Manihot Esculenta ◽  
Motor Coordination ◽  
Neuronal Damage ◽  
Motor Impairment ◽  
Hepatic Function ◽  
Hepatic Damage ◽  
Male Rats ◽  
Motor Impairments ◽  
Manihot Esculenta Crantz ◽  
Swim Test

Cassava (Manihot esculenta Crantz) is a plant that contains neurotoxins such as linamarin and lotaustraline. Its long-term consumption is associated with neuronal damage and contributes to the development of motor impairment in humans and rats. We investigated the effects of the consumption of cassava juice on renal and hepatic function and motor impairments in male rats. The rats received the vehicle, non-toxic and toxic doses of cassava juice, or linamarin as a pharmacological control, over 35 consecutive days. The effects were evaluated in an open field test, rotarod, and swim test. The toxic cassava dose and linamarin resulted in motor impairments in the rotarod and swim test from day 7 of treatment. The toxic cassava dose and linamarin increased the parameters that indicate renal and hepatic damage, with the exception of total protein and albumin levels. Behavioral variables that show motor incoordination (i.e., latency to fall in the rotarod) were negatively correlated with biochemical parameters of renal and kidney damage, whereas spin behavior was positively correlated. Our data indicate that chronic oral consumption of cassava juice caused renal and hepatic damage that was correlated with motor coordination impairment in rats, similarly to their principal neurotoxic compound, linamarin.

scholarly journals Oxytocin prevents ethanol actions at δ subunit-containing GABAA receptors and attenuates ethanol-induced motor impairment in rats

2015 ◽  
Vol 112 (10) ◽  
pp. 3104-3109 ◽  
Author(s):  
Michael T. Bowen ◽  
Sebastian T. Peters ◽  
Nathan Absalom ◽  
Mary Chebib ◽  
Inga D. Neumann ◽  
...  
Keyword(s):  
Motor Coordination ◽  
Alcohol Intoxication ◽  
Motor Impairment ◽  
Direct Interaction ◽  
Structural Similarity ◽  
Cellular Level ◽  
Male Rats ◽  
In Vitro Effects ◽  
Gabaergic Activity

Even moderate doses of alcohol cause considerable impairment of motor coordination, an effect that substantially involves potentiation of GABAergic activity at δ subunit-containing GABAA receptors (δ-GABAARs). Here, we demonstrate that oxytocin selectively attenuates ethanol-induced motor impairment and ethanol-induced increases in GABAergic activity at δ-GABAARs and that this effect does not involve the oxytocin receptor. Specifically, oxytocin (1 µg i.c.v.) given before ethanol (1.5 g/kg i.p.) attenuated the sedation and ataxia induced by ethanol in the open-field locomotor test, wire-hanging test, and righting-reflex test in male rats. Using two-electrode voltage-clamp electrophysiology in Xenopus oocytes, oxytocin was found to completely block ethanol-enhanced activity at α4β1δ and α4β3δ recombinant GABAARs. Conversely, ethanol had no effect when applied to α4β1 or α4β3 cells, demonstrating the critical presence of the δ subunit in this effect. Oxytocin had no effect on the motor impairment or in vitro effects induced by the δ-selective GABAAR agonist 4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol, which binds at a different site on δ-GABAARs than ethanol. Vasopressin, which is a nonapeptide with substantial structural similarity to oxytocin, did not alter ethanol effects at δ-GABAARs. This pattern of results confirms the specificity of the interaction between oxytocin and ethanol at δ-GABAARs. Finally, our in vitro constructs did not express any oxytocin receptors, meaning that the observed interactions occur directly at δ-GABAARs. The profound and direct interaction observed between oxytocin and ethanol at the behavioral and cellular level may have relevance for the development of novel therapeutics for alcohol intoxication and dependence.

CONCENTRADO PROTEICO OBTIDO DAS FOLHAS DE MANDIOCA (Manihot esculenta Crantz) DE TRÊS VARIEDADES COMERCIAIS.

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Izabel Aparecida Soares ◽  
Mauro Sérgio Téo ◽  
Carlise DEBASTIANI ◽  
Suzymeire BARONI ◽  
Vanessa Silva RETUCI
Keyword(s):  
Manihot Esculenta ◽  
Crude Protein ◽  
Hydrocyanic Acid ◽  
Protein Concentrate ◽  
Manihot Esculenta Crantz ◽  
White Variety ◽  
Mean Values ◽  
Randomized Design ◽  
Significant Difference ◽  
The Mean

O trabalho teve por objetivo verificar diferenças entre rendimento do concentrado proteico e proteína bruta da folha da mandioca (Manihot esculenta Crantz), obtidos a partir de três variedades comerciais: branca, cascuda e vermelha. As manivas foram plantadas seguindo o delineamento experimental inteiramente casualizado com três repetições. Nas comparações entre as variedades, considerou coletas escalonadas pós-plantio, realizadas aos 12, 14 e 16 meses. O concentrado proteico foi obtido a partir da farinha das folhas inteiras e submetido ao método de termo - coagulação ácido e a proteína bruta pelo método padrão AOAC. Os dados foram submetidos a análise de variância e comparados pelo teste de Tukey - 5% de probabilidade. Os resultados não indicaram diferença significativa entre as médias obtidas para rendimento de concentrado proteico. Para a variável porcentagem de proteína bruta a variedade Branca foi a que apresentou maior valor, com média de 46,25%, seguida pela Cascuda e Vermelha, 44,52% e 37,30%, sucessivamente. Conclui-se que outros estudos devem avaliar condições que possam influenciar no teor de proteína foliar, como clima e solo, e, avaliar os níveis de ácido cianídrico de cada variedade, indicando qual das três é a melhor para a extração do concentrado proteico das folhas. Palavras chaves: Manihot esculenta Crantz, variedades comerciais, concentrado proteico, proteína bruta. ABSTRACT: The study aimed to assess the differences between income protein concentrate, crude protein of cassava leaf (Manihot esculenta Crantz), obtained from three commercial varieties: white, red and cascuda. The cuttings were planted following the completely randomized design with three replications. Comparisons between the varieties considered after planting staggered collections, held on 12, 14 and 16 months. The protein concentrate was obtained from flour of whole sheets and subjected to the term method - acid coagulation and crude protein by AOAC standard method. The data were submitted to ANOVA and Tukey test - 5% probability. The results indicated no significant difference between the mean values obtained for protein concentrate income. For the variable percentage of crude protein White variety showed the highest, with an average of 46.25%, followed by cascuda and Red, 44.52% and 37.30%, successively. We conclude that further studies should evaluate conditions that may affect the leaf protein content, such as climate and soil, and evaluate the hydrocyanic acid levels of each variety, indicating which of the three is the best for the extraction of protein concentrate from leaves Key words: Manihot esculenta Crantz, commercial varieties, protein concentrate, crude protein.

MODELAGEM DA CINÉTICA DE SECAGEM EM FÉCULAS DE MANDIOCA (MANIHOT ESCULENTA CRANTZ)

2018 ◽  
Author(s):  
Barros Sâmela Leal ◽  
Santos Newton Carlos ◽  
Araújo Thaís Jaciane ◽  
Melo Mylena Olga Pessoa ◽  
Nascimento Amanda Priscila Silva
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

CONJUNTURA DO MERCADO DA MANDIOCA (Manihot esculenta Crantz) NO BRASIL E NO ESTADO DO PARÁ

2018 ◽  
Author(s):  
Brochado Maura Gabriela da Silva ◽  
Botelho Matheus Gabriel Lopes ◽  
Souza Camila Eduarda Sousa de ◽  
Nunes Mariana Lameira ◽  
Ferreira Leonardo Elias
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

Seleksi Ubi Kayu (Manihot esculenta Crantz.) Lokal Berdaya Hasil Tinggi Asal Indonesia berdasarkan Karakter Umbi

2018 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Fadhillah Laila ◽  
Budi Waluyo ◽  
Agung Kurniawan
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

Indonesia memiliki kekayaan plasma nutfah ubi kayu yang tersebar di seluruh wilayah Indonesia. Ubi kayu mengandung karbohidrat tinggi dan berperan dalam diversifikasi pangan. Tujuan dari penelitian ini adalah menyeleksi 80 varietas ubi kayu budidaya lokal asal berbagai wilayah di Indonesia yang berpotensi hasil tinggi. Penelitian ini menggunakan 80 aksesi ubi kayu dari seluruh pulau-pulau besar di Indonesia yang disusun dalam rancangan Augmented dengan 3 tanaman kontrol per baris. Penelitian dilakukan dari Juli 2013-November 2014. Hasil penelitian menunjukkan bahwa keragaman genetik dan fenotipik yang luas pada ubi kayu asal Indonesia berdasarkan karakter umbi. Potensi genetik berdasarkan karakter hasil dan komponen hasil terkategorikan tinggi dengan nilai heritabilitas 0,59-0,75. Uji lanjut LSI (Least Significant Increase) menyeleksi aksesi yang memiliki daya hasil tinggi pada karakter umbi diantaranya karakter jumlah ubi/tanaman pada aksesi 563, 570, dan 599. Karakter bobot ubi/tanaman pada aksesi 507,563, 598, dan 541. Karakter bobot/ubi pada aksesi aksesi 534,528 dan 541. Karakter bobot ubi/plot pada aksesi 629. Karakter potensi hasil pada aksesi 629. Hal ini dapat memberikan informasi mengenai diversitas genetik aksesi-aksesi ubi kayu lokal Indonesia sehingga dapat dievaluasi untuk didapatkan hasil tinggi ubi kayu untuk diversifikasi pangan.

scholarly journals Comprehensive genotyping of a Brazilian cassava (Manihot esculenta Crantz) germplasm bank: insights into diversification and domestication

2021 ◽  
Vol 134 (5) ◽  
pp. 1343-1362
Author(s):  
Alex C. Ogbonna ◽  
Luciano Rogerio Braatz de Andrade ◽  
Lukas A. Mueller ◽  
Eder Jorge de Oliveira ◽  
Guillaume J. Bauchet
Keyword(s):  
Population Genetics ◽  
Population Structure ◽  
Manihot Esculenta ◽  
Ex Situ ◽  
Germplasm Bank ◽  
Effective Population ◽  
Manihot Esculenta Crantz ◽  
Genetic Groups ◽  
Population Structure Analysis ◽  
Modern Breeding

Abstract Key message Brazilian cassava diversity was characterized through population genetics and clustering approaches, highlighting contrasted genetic groups and spatial genetic differentiation. Abstract Cassava (Manihot esculenta Crantz) is a major staple root crop of the tropics, originating from the Amazonian region. In this study, 3354 cassava landraces and modern breeding lines from the Embrapa Cassava Germplasm Bank (CGB) were characterized. All individuals were subjected to genotyping-by-sequencing (GBS), identifying 27,045 single-nucleotide polymorphisms (SNPs). Identity-by-state and population structure analyses revealed a unique set of 1536 individuals and 10 distinct genetic groups with heterogeneous linkage disequilibrium (LD). On this basis, a density of 1300–4700 SNP markers were selected for large-effect quantitative trait loci (QTL) detection. Identified genetic groups were further characterized for population genetics parameters including minor allele frequency (MAF), observed heterozygosity $$({H}_{o})$$ ( H o ) , effective population size estimate $$\widehat{{(N}_{e}}$$ ( N e ^ ) and polymorphism information content (PIC). Selection footprints and introgressions of M. glaziovii were detected. Spatial population structure analysis revealed five ancestral populations related to distinct Brazilian ecoregions. Estimation of historical relationships among identified populations suggests an early population split from Amazonian to Atlantic forest and Caatinga ecoregions and active gene flows. This study provides a thorough genetic characterization of ex situ germplasm resources from cassava’s center of origin, South America, with results shedding light on Brazilian cassava characteristics and its biogeographical landscape. These findings support and facilitate the use of genetic resources in modern breeding programs including implementation of association mapping and genomic selection strategies.

GC–MS analyses reveal chemical differences in the leaves of Manihot esculenta Crantz genotypes with different anti-herbivore effects

2021 ◽  
Author(s):  
Diandro Ricardo Barilli ◽  
Ilara Gabriela Frasson Budzinski ◽  
João Luiz Bronzel Junior ◽  
Vanderlan da Silva Bolzani ◽  
Arlindo Leal Boiça Júnior ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

Characterization of hemicellulose in Cassava (Manihot esculenta Crantz) stem during xylogenesis

2021 ◽  
Vol 264 ◽  
pp. 118038
Author(s):  
Haoqiang Yang ◽  
Na Yi ◽  
Shuai Zhao ◽  
Zhouyang Xiang ◽  
Mirza Faisal Qaseem ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz
Sign in / Sign up

Export Citation Format

Share Document