scholarly journals High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1343
Author(s):  
Nildimar Alves Honório ◽  
Daniel Cardoso Portela Câmara ◽  
Keenan Wiggins ◽  
Bradley Eastmond ◽  
Barry Wilmer Alto
Keyword(s):  
Aedes Aegypti ◽  
High Throughput ◽  
Filter Paper ◽  
Detection Method ◽  
Capillary Tube ◽  
Vector Competence ◽  
Detection Methods ◽  
High Throughput Method ◽  
Mosquito Saliva ◽  
High Throughput Detection

Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is time-consuming and labor-intensive. Here we compare the capillary tube method to an alternative high-throughput detection method the collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper) reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus increased the odds of females having a positive salivary infection and higher salivary viral titers, while for the IOL genotype the same effect was observed only for Ae. aegypti. Of the three tested detection methods, the FTA card was significantly more effective at detecting infected saliva of Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as effective as the capillary tube for the Asian genotype. We did not find significant effects of the detection method in detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the limitations of the different tested detection methods.

scholarly journals High-throughput Detection Method for Influenza Virus

10.3791/3623 ◽  
2012 ◽  
Author(s):  
Pawan Kumar ◽  
Allison E. Bartoszek ◽  
Thomas M. Moran ◽  
Jack Gorski ◽  
Sanjib Bhattacharyya ◽  
...  
Keyword(s):  
Influenza Virus ◽  
High Throughput ◽  
Detection Method ◽  
High Throughput Detection

High-throughput detection method of quorum-sensing molecules by colorimetry and its applications

2006 ◽  
Vol 356 (2) ◽  
pp. 297-299 ◽  
Author(s):  
Yung-Hun Yang ◽  
Tek-Hyung Lee ◽  
June Hyung Kim ◽  
Eun Jung Kim ◽  
Hwang-Soo Joo ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
High Throughput ◽  
Detection Method ◽  
High Throughput Detection

A New, Easy, and Rapid High-Throughput Detection Method for the Common GJB2 (CX26), 35delG Mutation

2007 ◽  
Vol 11 (3) ◽  
pp. 231-234 ◽  
Author(s):  
E. Van Eyken ◽  
G. Van Camp ◽  
J.J. Hendrickx ◽  
K. Demeester ◽  
A. Vandevelde ◽  
...  
Keyword(s):  
High Throughput ◽  
Detection Method ◽  
High Throughput Detection ◽  
The Common ◽  
35Delg Mutation

scholarly journals Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release

2021 ◽  
Vol 15 (9) ◽  
pp. e0009752
Author(s):  
Nicolas Pocquet ◽  
Olivia O’Connor ◽  
Heather A. Flores ◽  
Jordan Tutagata ◽  
Morgane Pol ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cytoplasmic Incompatibility ◽  
New Caledonia ◽  
Vector Competence ◽  
Oral Feeding ◽  
Maternal Transmission ◽  
Strong Inhibitor ◽  
Feeding Experiments ◽  
Dengue Outbreaks ◽  
Mosquito Saliva

Background Biological control programs involving Wolbachia-infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue virus (DENV) is Ae. aegypti and dengue outbreaks frequency and severity are increasing. We report the generation of a NC Wolbachia-infected Ae. aegypti strain and the results of experiments to assess the vector competence and fitness of this strain for future implementation as a disease control strategy in Noumea, NC. Methods/principal findings The NC Wolbachia strain (NC-wMel) was obtained by backcrossing Australian AUS-wMel females with New Caledonian Wild-Type (NC-WT) males. Blocking of DENV, chikungunya (CHIKV), and Zika (ZIKV) viruses were evaluated via mosquito oral feeding experiments and intrathoracic DENV challenge. Significant reduction in infection rates were observed for NC-wMel Ae. aegypti compared to WT Ae. aegypti. No transmission was observed for NC-wMel Ae. aegypti. Maternal transmission, cytoplasmic incompatibility, fertility, fecundity, wing length, and insecticide resistance were also assessed in laboratory experiments. Ae. aegypti NC-wMel showed complete cytoplasmic incompatibility and a strong maternal transmission. Ae. aegypti NC-wMel fitness seemed to be reduced compared to NC-WT Ae. aegypti and AUS-wMel Ae. aegypti regarding fertility and fecundity. However further experiments are required to assess it accurately. Conclusions/significance Our results demonstrated that the NC-wMel Ae. aegypti strain is a strong inhibitor of DENV, CHIKV, and ZIKV infection and prevents transmission of infectious viral particles in mosquito saliva. Furthermore, our NC-wMel Ae. aegypti strain induces reproductive cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, supporting field-releases in Noumea, NC.

A high-throughput detection method for invasive fruit fly (Diptera: Tephritidae) species based on microfluidic dynamic array

2016 ◽  
Vol 16 (6) ◽  
pp. 1378-1388 ◽  
Author(s):  
Fan Jiang ◽  
Wei Fu ◽  
Anthony R. Clarke ◽  
Mark Kurt Schutze ◽  
Agus Susanto ◽  
...  
Keyword(s):  
High Throughput ◽  
Detection Method ◽  
Fruit Fly ◽  
Dynamic Array ◽  
High Throughput Detection

scholarly journals Sialic Acid Expression in the MosquitoAedes aegyptiand Its Possible Role in Dengue Virus-Vector Interactions

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Jorge Cime-Castillo ◽  
Philippe Delannoy ◽  
Guillermo Mendoza-Hernández ◽  
Verónica Monroy-Martínez ◽  
Anne Harduin-Lepers ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Mammalian Cells ◽  
Vector Competence ◽  
Denv Infection ◽  
Viral Disease ◽  
Salivary Gland Extract ◽  
Denv Serotypes ◽  
Mosquito Saliva

Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquitoAedes aegyptithat has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) inAedes aegyptitissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA forAedes aegyptiCMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells.AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related toα-2,6-sialyltransferase were detected in theAedes aegyptigenome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission.

scholarly journals High Throughput Detection Methods for Multiplex Mycotoxins

2017 ◽  
Vol 03 (03) ◽  
Author(s):  
Jianlin Li ◽  
Yang Deng ◽  
Yan Liu ◽  
Zhi Ding ◽  
Yichen Li ◽  
...  
Keyword(s):  
High Throughput ◽  
Detection Methods ◽  
High Throughput Detection
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