scholarly journals Selection for and Analysis of UV-Resistant Cryptophlebia Leucotreta Granulovirus-SA as a Biopesticide for Thaumatotibia leucotreta

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 28
Author(s):  
Patrick Mwanza ◽  
Michael Jukes ◽  
Gill Dealtry ◽  
Michael Lee ◽  
Sean Moore
Keyword(s):  
Uv Irradiation ◽  
Codling Moth ◽  
Probit Analysis ◽  
Uv Exposure ◽  
Thaumatotibia Leucotreta ◽  
Uv Tolerance ◽  
False Codling Moth ◽  
Selection For ◽  
Cryptophlebia Leucotreta

Cryptophlebia leucotreta granulovirus-SA (CrleGV-SA) is used as a commercial biopesticide for the false codling moth, Thaumatotibia leucotreta, in citrus and other crops. The virus is sensitive to UV irradiation from sunlight, which reduces its efficacy as a biopesticide in the field. We selected a UV-resistant CrleGV-SA isolate, with more than a thousand-fold improved virulence compared to the wild-type isolate, measured by comparing LC50 values. CrleGV-SA purified from infected T. leucotreta larvae was exposed to UV irradiation under controlled laboratory conditions in a climate chamber mimicking field conditions. Five cycles of UV exposure, followed by propagating the virus that retained infectivity in vivo with re-exposure to UV, were conducted to isolate and select for UV-resistant virus. Serial dilution bioassays were conducted against neonates after each UV exposure cycle. The concentration-responses of the infectious UV-exposed virus populations were compared by probit analysis with those from previous cycles and from the original CrleGV-SA virus population. NGS sequences of CrleGV-SA samples from UV exposure cycle 1 and cycle 5 were compared with the GenBank CrleGV-SA sequence. Changes in the genomes of infective virus from cycles 1 and 5 generated SNPs thought to be responsible for establishing UV tolerance. Additional SNPs, detected only in the cycle 5 sequence, may enhance UV tolerance and improve the virulence of the UV-tolerant population.

False codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae) larvae are chill-susceptible

2011 ◽  
Vol 19 (3) ◽  
pp. 315-328 ◽  
Author(s):  
Leigh Boardman ◽  
Tim G. Grout ◽  
John S. Terblanche
Keyword(s):  
Codling Moth ◽  
Thaumatotibia Leucotreta ◽  
False Codling Moth

Movement and impact of Trichogrammatoidea cryptophlebiae Nagaraja (Hymenoptera: Trichogrammatidae) in citrus orchards after inundative releases against the false codling moth, Cryptophlebia leucotreta (Meyrick) (Lepidoptera: Tortricidae)

1988 ◽  
Vol 78 (1) ◽  
pp. 85-99 ◽  
Author(s):  
Philip J. Newton
Keyword(s):  
Codling Moth ◽  
Egg Parasitoid ◽  
Linear Increase ◽  
Crop Damage ◽  
Larval Population ◽  
Important Pest ◽  
False Codling Moth ◽  
Pest Density ◽  
Cryptophlebia Leucotreta ◽  
Inundative Releases

AbstractInundative releases of the indigenous egg parasitoid Trichogrammatoidea cryptophlebiae Nagaraja were made against Cryptophlebia leucotreta (Meyrick) in two sweet orange orchards in the Transvaal lowveld, South Africa. The rate of parasitism was raised to similar levels in the two release areas, but there was a significant decrease in crop damage in only one of them. Although the larval population was twice as large in a control orchard, the losses there were compensated for by a larger total crop. Three explanations for the different performances of the parasitoids are examined. Movement away from release areas was not apparent. Rates of parasitism at the tops or bottoms of trees or at the four cardinal aspects were not significantly different overall but should have been to compensate for the linear increase in numbers of host eggs with height, and the larger numbers on the warmer northern and eastern sides of trees. Parasitism was higher at the tree tops than at the bottoms in the orchard that lost the least fruit. The stochastic processes governing host-egg distribution on fruit were also important. Pest density was higher in the release areas than the control, and eggs were distributed more contagiously in the orchard with the most crop loss.

Development of reduced cost diets for the mass rearing of false codling moth ( Thaumatotibia leucotreta )

2019 ◽  
Vol 99 (8) ◽  
pp. 3976-3982 ◽  
Author(s):  
Michael J Woods ◽  
Nevil Boersma ◽  
Louwrens C Hoffman ◽  
Elsje Pieterse
Keyword(s):  
Codling Moth ◽  
Mass Rearing ◽  
Thaumatotibia Leucotreta ◽  
False Codling Moth

scholarly journals Thermal limits to survival and activity in two life stages of false codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae)

2017 ◽  
Vol 42 (4) ◽  
pp. 379-388 ◽  
Author(s):  
John S. Terblanche ◽  
Katherine A. Mitchell ◽  
Wilmari Uys ◽  
Clancy Short ◽  
Leigh Boardman
Keyword(s):  
Codling Moth ◽  
Life Stages ◽  
Thermal Limits ◽  
Thaumatotibia Leucotreta ◽  
False Codling Moth

scholarly journals First report of Trichogramma danausicida and Trichogramma cacaeciae reared from Thaumatotibia leucotreta eggs in Israel

ZooKeys ◽  
2018 ◽  
Vol 779 ◽  
pp. 19-25 ◽  
Author(s):  
Roy Kaspi ◽  
Svetlana Kontsedalov ◽  
Murad Ghanim
Keyword(s):  
Coastal Plain ◽  
Field Survey ◽  
Codling Moth ◽  
Parasitoid Species ◽  
First Report ◽  
Thaumatotibia Leucotreta ◽  
False Codling Moth ◽  
Gene Coi ◽  
First Time ◽  
I Gene

The egg parasitpoids Trichogrammadanausicida (Nagaraja) and Trichogrammacacaeciae (Marchal) (Hymenoptera: Trichogrammatidae), are reported for the first time in Israel. Moreover, our discovery of T.danausicida is the first report of this parasitoid species outside of India. The occurrence of those trichogrammatids was first discovered and documented in May 2016 during a survey of egg parasitoids of the False codling moth Thaumatotibialeucotreta (Lepidoptera: Tortricidae). The field survey was conducted on castor bean fruits (Ricinuscommunis) in the Israeli central coastal plain. The identity of the parasitoids was revealed by means of sequencing a portion of the cytochrome oxidase I gene (COI) of the studied parasitoids.

Sign in / Sign up

Export Citation Format

Share Document