scholarly journals A Cross Sectional Sampling Reveals Novel Coronaviruses in Bat Populations of Georgia

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 72
Author(s):  
Lela Urushadze ◽  
George Babuadze ◽  
Mang Shi ◽  
Luis E. Escobar ◽  
Matthew R. Mauldin ◽  
...  

Mammal-associated coronaviruses have a long evolutionary history across global bat populations, which makes them prone to be the most likely ancestral origins of coronavirus-associated epidemics and pandemics globally. Limited coronavirus research has occurred at the junction of Europe and Asia, thereby investigations in Georgia are critical to complete the coronavirus diversity map in the region. We conducted a cross-sectional coronavirus survey in bat populations at eight locations of Georgia, from July to October of 2014. We tested 188 anal swab samples, remains of previous pathogen discovery studies, for the presence of coronaviruses using end-point pan-coronavirus RT-PCR assays. Samples positive for a 440 bp amplicon were Sanger sequenced to infer coronavirus subgenus or species through phylogenetic reconstructions. Overall, we found a 24.5% positive rate, with 10.1% for Alphacoronavirus and 14.4% for Betacoronavirus. Albeit R. euryale, R. ferrumequinum, M. blythii and M. emarginatus were found infected with both CoV genera, we could not rule out CoV co-infection due to limitation of the sequencing method used and sample availability. Based on phylogenetic inferences and genetic distances at nucleotide and amino acid levels, we found one putative new subgenus and three new species of Alphacoronavirus, and two new species of Betacoronavirus.

Zootaxa ◽  
2019 ◽  
Vol 4583 (1) ◽  
pp. 1
Author(s):  
W.F. PONDER ◽  
W.-H. ZHANG ◽  
A. HALLAN ◽  
M.E. SHEA

Species from artesian springs associated with the Queensland Great Artesian Basin that were previously included in the tateid genus Jardinella are included in three new genera, namely Eulodrobia, with six species, five of them new and all from the Eulo Supergroup; Springvalia, with one species from the Springvale Supergroup; and Carnarvoncochlea with two previously-described species, from the Carnarvon Supergroup. The genus Edgbastonia is extended to include eight previously described species, in addition to the type species, and four new species-group taxa from the Barcaldine Supergroup springs; all but the type species are included in the new subgenus Barcaldinia. Three new species from non-artesian springs in north Queensland are included in Edgbastonia, one of them tentatively. Two additional related new genera, both with a single new species, are described from outside the Great Artesian Basin; Conondalia from southeast Queensland and Nundalia from north-eastern New South Wales. The genus Jardinella, previously used for all the Queensland spring tateids, is here restricted to three species found in coastal rivers and streams in northeast Queensland. A molecular phylogenetic analysis using COI and 16S mitochondrial genes in combination suggests that the Queensland Great Artesian Basin taxa may be more closely related to the tateid genera Austropyrgus, Pseudotricula, Posticobia and Potamopyrgus than to the South Australian GAB taxa, thus indicating the separate origins of these two desert spring faunas. 


Zootaxa ◽  
2014 ◽  
Vol 3795 (4) ◽  
pp. 401 ◽  
Author(s):  
SERGEY G. ERMILOV ◽  
UMUKUSUM YA. SHTANCHAEVA ◽  
LUIS S. SUBÍAS ◽  
ALEXANDER E. ANICHKIN

Crustaceana ◽  
2013 ◽  
Vol 86 (2) ◽  
pp. 182-211 ◽  
Author(s):  
Janet Higuti ◽  
Isa Schön ◽  
Leen Audenaert ◽  
Koen Martens

We redescribe Strandesia tolimensis Roessler, 1990, originally described from Colombia, and describe three new species, Strandesia lansactohai n. sp., S. velhoi n. sp. and S. nupelia n. sp. from lakes, channels and rivers in the alluvial valley of the upper Paraná River. Mainly valve morphology and shape and size of the attachment of the caudal ramus are used to characterize these species, as other soft parts are uninformative. All populations studied here are parthenogenetic, so male morphologies are not available. Sequences of the mitochondrial COI-gene are used to construct a phylogeny, as well as a genetic network of specimens, and in both analyses the four species are well-supported. In addition, we also delimit the four species with genetic distances using Birky’s K/θ method and conclude that it supports the identity of the species under consideration.


ZooKeys ◽  
2021 ◽  
Vol 1037 ◽  
pp. 57-71
Author(s):  
Zhaoyang Chen ◽  
Dengqing Li ◽  
Daiqin Li ◽  
Xin Xu

We diagnose and describe three new species of the primitively segmented spider genus Songthela from Guizhou Province, China, based on morphological characters and molecular data: S. liuisp. nov. (♂♀), S. tianzhusp. nov. (♂♀), and S. yupingsp. nov. (♂♀). We provide the genetic distances within and among the three new species based on the DNA barcode gene, cytochrome c oxidase subunit I (COI) to support our descriptions. We also provide the COI GenBank accession codes for the three new species for future identification.


Zootaxa ◽  
2008 ◽  
Vol 1876 (1) ◽  
pp. 19 ◽  
Author(s):  
VLADIMIR KONONENKO ◽  
MICHAEL FIBIGER

Based on the collection of the Zoological Research Institute and Museum Alexander Koenig, Germany, Bonn, two new species of the genera Lygephila Billberg, 1820 and Apopestes Hübner, [1823] are described from China. The new taxa belong to the subfamily Catocalinae, tribe Toxocampini: Lygephila stueningi, sp. n., Lygephila longicoecum sp. n., and Apopestes curiosa sp. n. The new subgnenus Sinocampa, subgen. n. (type species Lygephila longicoecum, sp. n.) of the genus Lygephila is described. The adults and male and female genitalia are illustrated, and distribution maps of the new species are presented.


Zootaxa ◽  
2006 ◽  
Vol 1297 (1) ◽  
pp. 23 ◽  
Author(s):  
YVES BOUSQUET ◽  
REGINALD WEBSTER

Three new species of Bembidion Latreille occurring in Canada are described: B. (Semicampa) nigrivestis Bousquet, sp. nov., type locality: Sainte-Catherine-de-Hatley (45°15'N, 72°03'W), Quebec; B. (Eupetedromus) iridipenne Bousquet and Webster, sp. nov., type locality: Lincoln (45° 49'N, 66°35'W), New Brunswick; and B. (Neobembidion) nitidicolle Bousquet, sp. nov., type locality: Rosefield (49°05'N, 107°32'W), Saskatchewan. A new subgenus, Neobembidion Bousquet, subgen. nov. (type species: Bembidion constricticolle Hayward, 1897), is proposed for the species of the constricticolle group as defined by Lindroth. Keys are included to distinguish the new species from their North American relatives.


Zootaxa ◽  
2020 ◽  
Vol 4896 (3) ◽  
pp. 409-442 ◽  
Author(s):  
TOUFIEK SAMAAI ◽  
MICHELLE KELLY ◽  
BENEDICTA NGWAKUM ◽  
ROBYN PAYNE ◽  
PETER R. TESKE ◽  
...  

Sixteen species of Latrunculiidae Topsent, 1922, belonging to the genera Latrunculia du Bocage, 1869, Strongylodesma Lévi, 1969, Cyclacanthia Samaai & Kelly, 2004, Samaai & Kelly, 2002, are currently known from the temperate waters of South Africa. Extensive new sponge collections from the Amathole region of South Africa revealed the existence of three new species of Tsitsikamma, T. amatholensis sp. nov., T. madiba sp. nov., and T. beukesi sp. nov., and a new species of the endemic South African genus Cyclacanthia, C. rethahofmeyri sp. nov. With the recent addition of two new species of Tsitsikamma from Algoa Bay and Tsitsikamma National Park (T. michaeli Parker-Nance, 2019; T. nguni Parker-Nance, 2019) the total number of known South African Latrunculiidae is now 20 species in four genera. Here we propose two new subgenera of Tsitsikamma, Tsitsikamma Samaai & Kelly, 2002 and Clavicaulis subgen. nov., based on the morphological groups “favus” and “pedunculata” hypothesized by Parker-Nance et al. (2019). Species in the nominotypical subgenus Tsitsikamma, containing the type species, are thick encrusting to hemispherical with a rigid honeycombed choanosome, while species in the new subgenus Clavicaulis subgen. nov. have a purse or sac-like morphology with little choanosomal structure. Despite the obvious species-level differences in morphology, multivariate analysis based on spicule measurements (anisostyle length, discorhabd length, shaft and whorl length) was not able to distinguish between the proposed Tsitsikamma species, but separated known species T. favus Samaai & Kelly, 2002, T. pedunculata Samaai & Kelly, 2003, and T. scurra Samaai & Kelly, 2003, from each other. Similarly, DNA barcoding of the mitochondrial COI  and the nuclear ITS of Tsitsikamma specimens failed to clearly differentiate between species, but was able to differentiate sister taxon relationships within the Latrunculiidae. 


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