scholarly journals Development and Validation of Vierordt’s and Absorbance Ratio Spectrophotometric Methods for Simultaneous Estimation of Aspirin and Omeprazole in Their Binary Mixture

2020 ◽  
Vol 3 (3) ◽  
pp. 328-335
Keyword(s):  
Binary Mixture ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Absorbance Ratio ◽  
Development And Validation

scholarly journals Development and validation of analytical methods for the simultaneous estimation of Nimorazole and Ofloxacin in tablet dosage form

2016 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
A P Ghugare ◽  
Lalchand Dayanand Devhare ◽  
B P Hatwar
Keyword(s):  
Analytical Methods ◽  
Dosage Form ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Distilled Water ◽  
Spectrophotometric Methods ◽  
Ratio Equation ◽  
Absorbance Ratio ◽  
Development And Validation ◽  
Tablet Dosage

<p>Two simple, rapid, accurate and precise spectrophotometric methods have been developed for simultaneous estimation of Nimorazole and Ofloxacin from tablet dosage form. Method І involves formation of ‘simultaneous equations’ at 304 nm (λ max of Nimorazole) and 287.5 nm (λ max of Ofloxacin); while Method ІІ involves, formation of ‘Absorbance ratio equation’ at 301(isoabsorptive point) and 287.5 nm (λ max of Ofloxacin) using distilled water as a solvent. The linearity was observed in the concentration range of 5 - 25 μg/ml for Nimorazole and 2 - 10 μg/ml for Ofloxacin. The results of analysis have been validated statistically and by recovery studies and were found satisfactory.</p>

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monika L. Jadhav ◽  
Manoj V. Girase ◽  
Shripad K. Tidme ◽  
Manish S. Junagade
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Absorbance Ratio ◽  
Standard Additions ◽  
Development And Validation ◽  
Tablet Dosage

Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form. The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, λmax for valsartan and hydrochlorothiazide, respectively. The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm (λmax of hydrochlorothiazide). The methods were found to be linear between the range of 5–30 µg/mL for valsartan and 4–24 μg/mL for hydrochlorothiazide using 0.1 N NaOH as solvent. The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions. The precision (intraday, interday) of methods was found within limits (RSD<2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.

scholarly journals A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Gitu Pandey ◽  
Brahmeshwar Mishra
Keyword(s):  
Method Development ◽  
The Other ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Ratio Analysis ◽  
Absorption Ratio ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

A new UV spectrophotometric absorption ratio method was developed and validated for the simultaneous estimation of lamivudine and isoniazid. The method involved Q-absorption ratio analysis using two wavelengths, with one being the λmax of lamivudine (272 nm, λ2) and the other being the isoabsorptive point of both drugs (246 nm, λ1). Beer’s law was obeyed in the concentration range between 5 and 30 µg/mL for both lamivudine and isoniazid. The results of analysis have been validated statistically and by recovery studies as per ICH guidelines. The accuracy ranged between 99.65 and 101.91% and Sandell’s sensitivity ranged between 0.0229 and 0.0347 µg/cm2. The method was found to be simple, precise, reproducible, rapid, and economical. Hence, it could be used in the analysis of laboratory samples and marketed formulations containing these two drugs in the future.

DEVELOPMENT AND VALIDATION OF A UV SPECTROPHOTOMETRIC Q-ABSORBANCE RATIO METHOD FOR SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 50-56
Author(s):  
M. Simoes ◽  
◽  
L. Almeida
Keyword(s):  
Dosage Form ◽  
Limit Of Detection ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Development And Validation ◽  
Tablet Dosage

A simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method has been developed for the simultaneous estimation of eperisone hydrochloride and aceclofenac in combined dosage form. The solvent used was methanol:water (70:30 v/v). The iso-absorptive point was found to be 269.5 nm. Calibration curves were linear over a concentration range of 6-21 μg/ml for eperisone hydrochloride and 8-28 μg/mL for aceclofenac, respectively. The developed method was validated as per International Conference on Harmonization (ICH) guidelines for various parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation. Accuracy of method was determined through recovery studies which were found to be 99.0% - 100.89 % for eperisone hydrochloride and 98.67% - 100.44 % for aceclofenac. Method was found to be reproducible with relative standard deviation (RSD) for intra-and inter-day precision less than 2% over the concentration range. The proposed method can be used for routine analysis of eperisone hydrochloride and aceclofenac in bulk and tablet dosage form.

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