Development and Validation of UV Spectrophotometric Methods for Simultaneous Estimation of Amlodipine besylate and Theophylline

2017 ◽  
Vol 7 (3) ◽  
pp. 159
Author(s):  
Ramesh L Sawant ◽  
Rahul B Kale ◽  
Amol S Ghodechor ◽  
Abhijit B Sherkar ◽  
Kunal R Sanklecha
Keyword(s):  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Spectrophotometric Methods ◽  
Development And Validation

scholarly journals Method development and validation for simultaneous estimation of amlodipine besylate and enalapril maleate in solid dosage form

2021 ◽  
Vol 8 (3) ◽  
pp. 129-133
Author(s):  
Manisha Masih
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Solution Stability ◽  
Amlodipine Besylate ◽  
Enalapril Maleate ◽  
Solid Dosage ◽  
Development And Validation ◽  
Liquid Chromatographic

A rapid, sensitive, specific, accurate and precise high pressure liquid chromatographic method (HPLC) method involving UV detection has been developed for the determination and quantification of Amlodipine Besylate and Enalapril maleate in bulk and combined dosage form. The determination was carried out on a Phenomenex C18 column (Dimention : 250 x 4.6 mm, 5 μm). The sample was analysed using filtered and degassed mixture of methanol : 0.1N HCl (1:1) as mobile phase at a flow rate of 1ml/min and effluent was monitored at 218nm. The retention time for Amlodipine besylate was 7.6 min and for Enalapril maleate 3.2 min. Amlodipine besylate and Enalapril maleate showed a linear response in the concentration range of 10-50μg/ml. The correlation co-efficient ('r' value) for Amlodipine besylate and Enalapril maleate was 0.9992 and 0.9994, respectively. The method was validated in terms of linearity, precision, accuracy, specificity, robustness and solution stability. The proposed method can be used for routine analysis of Amlodipine Besylate and Enalapril maleate in bulk and combined dosage form

scholarly journals Development and validation of analytical methods for the simultaneous estimation of Nimorazole and Ofloxacin in tablet dosage form

2016 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
A P Ghugare ◽  
Lalchand Dayanand Devhare ◽  
B P Hatwar
Keyword(s):  
Analytical Methods ◽  
Dosage Form ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Distilled Water ◽  
Spectrophotometric Methods ◽  
Ratio Equation ◽  
Absorbance Ratio ◽  
Development And Validation ◽  
Tablet Dosage

<p>Two simple, rapid, accurate and precise spectrophotometric methods have been developed for simultaneous estimation of Nimorazole and Ofloxacin from tablet dosage form. Method І involves formation of ‘simultaneous equations’ at 304 nm (λ max of Nimorazole) and 287.5 nm (λ max of Ofloxacin); while Method ІІ involves, formation of ‘Absorbance ratio equation’ at 301(isoabsorptive point) and 287.5 nm (λ max of Ofloxacin) using distilled water as a solvent. The linearity was observed in the concentration range of 5 - 25 μg/ml for Nimorazole and 2 - 10 μg/ml for Ofloxacin. The results of analysis have been validated statistically and by recovery studies and were found satisfactory.</p>

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