Extracellular membrane vesicles from umbilical cord blood-derived MSC protect against ischemic acute kidney injury, a feature that is lost after inflammatory conditioning

Background: The aim of this study was to investigate the relationship between umbilical cord blood neutrophil gelatinase-associated lipocalin and neonatal diseases, such as acute kidney injury. Materials and Method: The neutrophil gelatinase-associated lipocalin levels were measured in umbilical cord blood of 180 babies born in the department of Obstetrics and Gynecology between 2015-2016. Patients were classified according to maternal diseases, neonatal diseases, and demographic features. The obtained data were compared with umbilical cord blood neutrophil gelatinase-associated lipocalin levels. Results: There was a statistically significant difference between umbilical cord blood NGAL levels and premature rupture of membranes (p<0.05), ABO incompatibility (p<0.05), meconium aspiration syndrome (p<0.001), ventricular septal defect (p<0.001), and breech presentation birth (p<0.001). Conclusion: neutrophil gelatinase-associated lipocalin can be useful as a diagnostic biomarker in the evaluation of maternal and neonatal diseases. However, further studies on larger patient populations are needed.

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Endothelial Progenitor Cells Derived from Wharton's Jelly of Human Umbilical Cord Attenuate Ischemic Acute Kidney Injury by Increasing Vascularization and Decreasing Apoptosis, Inflammation, and Fibrosis

Cell Transplantation ◽

10.3727/096368914x681720 ◽

2015 ◽

Vol 24 (7) ◽

pp. 1363-1377 ◽

Cited By ~ 19

Author(s):

Chan-Jung Liang ◽

Wen-Ching Shen ◽

Fu-Bin Chang ◽

Vin-Cent Wu ◽

Shu-Huei Wang ◽

...

Keyword(s):

Acute Kidney Injury ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Umbilical Cord ◽

Kidney Injury ◽

Wharton’S Jelly ◽

Human Umbilical Cord ◽

Endothelial Progenitor ◽

Wharton's Jelly ◽

Ischemic Acute Kidney Injury

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Exvivo Experiments of Human Ovarian Cancer Ascites-Derived Exosomes Presented by Dendritic Cells Derived from Umbilical Cord Blood for Immunotherapy Treatment

Clinical medicine Oncology ◽

10.4137/cmo.s776 ◽

2008 ◽

Vol 2 ◽

pp. CMO.S776 ◽

Cited By ~ 3

Author(s):

Qi-Ling Li ◽

Ning Bu ◽

Yue-Cheng Yu ◽

Wei Hua ◽

Xiao-Yan Xin

Keyword(s):

Ovarian Cancer ◽

T Cells ◽

Dendritic Cells ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Tumor Antigens ◽

Specific Antigen ◽

Membrane Vesicles ◽

Hematopoietic Stem

Objectives Exosomes, a type of membrane vesicles, released from tumor cells have been shown to be capable of transferring tumor antigens to dendritic cells and activating specific cytotoxic T-lymphocytes. Recent work has demonstrated the presence of high numbers of exosomes in malignant effusions. Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells and from which a significant number of dendritic cells can be produced. We hypothesized that the exosomes released from metastatic ovarian carcinoma were able to present tumor specific antigen to dendritic cells derived from unrelated umbilical cord blood, then could stimulate resting T cells to differentiate and induce effective cytotoxicity. Study Design Exosomes were isolated by ultracentrifugation of malignant ascites from ovarian cancer patients (n = 10). Purified exosomes were further characterized by Western blot analyses and immunoelectronic microscopy. Dendritic cells were collected from unrelated umbilical cord blood and cultured in the presence of GM-CSF, IL-4 and TNF-α. Resting T cells were mixed with dentritic cells previously primed with exosomes and the cytotoxicity were measured by MTT method. T cells were activated by DCs presented with exosomes. Results 1) the exosomes isolated from the ascites were membrane vesicles of about 30-90nm in diameter; 2) the exosomes expressed MHC class I molecules, HSP70, HSP90, Her2/Neu, and Mart1; and 3)umbilical cord blood-derived DCs previously exosome-primed stimulated resting T cells to differentiate and produce effective cytotoxicity. Conclusions These results suggested that tumor-specific antigens present on exosomes can be presented by DCs derived from unrelated umbilical cord blood to induce tumor specific cytotoxicity and this may represent as a novel immunotherapy for ovarian cancer.

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