Coffee and coffee products. Determination of the caffeine content using high performance liquid chromatography (HPLC). Reference method

2015 ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 663-666
Author(s):  
G. M. M. Anwarul Hasan ◽  
Anuj Kumer Das

Caffeine, a phycostimulant is present in several foods and drinks.In the present study, beverages of different brands in Bangladeshi market were analyzed for caffeine by high-performance liquid chromatography (HPLC) using methanol-water (40:60, v/v) as mobile phase. Caffeine content ranged from 16.33 -19.33 mg/can in soft drinks and 45.66-47.33 mg/can in energy drinks respectively. These data indicated that the levels of caffeine in Bangladeshi soft drinks and energy drinks are within the ranges reported from similar products in other countries.


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