Single Nucleotide Polymorphisms in Tumor Necrosis Factor-α and Susceptibility to HIV Infection in Local Population of Lahore Pakistan

2020 ◽  
pp. 41-47
Keyword(s):  
Tumor Necrosis Factor ◽  
Hiv Infection ◽  
Promoter Region ◽  
Tumor Necrosis ◽  
Local Population ◽  
Nucleotide Polymorphisms ◽  
Chi Square ◽  
Single Nucleotide ◽  
Tnf Α ◽  
Necrosis Factor

A single nucleotide polymorphism (SNP) is observed at -308 position of the promoter region of tumor necrosis factor (TNF- α) gene due to which TNF is categorized into TNF1 and TNF2 allele. TNF2 allele is associated with higher concentration of TNF- α which in turn is associated with HIV infection. In order to know the association between TNF2 and HIV infection n =75 HIV positive samples and n=15 HIV negative samples were observed for TNF polymorphism. It was found that among the infected patients 53 patients had TNF2.The total percentage of the patients and controls having TNF2 allele was found to be 63.34.%. Chi square value was significant showing that there is a strong correlation between HIV susceptibility and TNF SNPs (-308) of the promoter region.

scholarly journals Associations between tumor necrosis factor-α and lymphotoxin-α polymorphisms and idiopathic recurrent miscarriage

Reproduction ◽  
2008 ◽  
Vol 135 (3) ◽  
pp. 397-403 ◽  
Author(s):  
W Zammiti ◽  
N Mtiraoui ◽  
H Khairi ◽  
J-C Gris ◽  
W Y Almawi ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Recurrent Miscarriage ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Tnf Α ◽  
Idiopathic Recurrent Miscarriage ◽  
Factor Α ◽  
Lymphotoxin Α ◽  
Necrosis Factor

Heightened expression of tumor necrosis factor (TNF)-α and lymphotoxin-α (LT-α) was associated with pregnancy complications, including idiopathic recurrent miscarriage (RM). Whereas TNF-α and LT-α gene polymorphisms affect serum cytokine concentrations, their contribution to RM is controversial. The single nucleotide polymorphisms (SNPs) TNF-α (−238G/A, −308G/A) and LT-α (+252A/G) were investigated in 350 RM women and 200 control women. Higher frequency of the TNF-α −238A, but not the TNF-α −308A or the LT-α+252G, allele was seen in patients, with comparable frequencies of TNF-α −238G/A, TNF-α −308G/A, and LT-α+252A/G genotypes seen between both groups, except for TNF-α −238G/G, which was lower in patients. Regression analysis confirmed the association of the TNF-α −238G/A SNP with idiopathic RM, and both TNF-α −308A/TNF −238G/LT-α+252Gand TNF-α −308G/TNF-α −238A/LT-α+252Ghaplotypes played a susceptible role in idiopathic RM. TNF-α −238G/A and −238A/A, and LT-α+252G/G genotypes were positively associated only with exclusively early RM. This supports the concept of the association of TNF-α (−238G/A) and LT-α (+252A/G) polymorphic variants in idiopathic RM.

scholarly journals Tumor Necrosis Factor Alpha-863 C/A Single Nucleotide Polymorphisms and Nephrotic Syndrome

2020 ◽  
Vol 10 (03) ◽  
pp. 319-322
Author(s):  
Ahmed Abdul-Hassan Abbas ◽  
Zainab J. Fadhil ◽  
Shatha Hussein Ali
Keyword(s):  
Nephrotic Syndrome ◽  
Tumor Necrosis Factor ◽  
Serum Level ◽  
Tumor Necrosis ◽  
Nucleotide Polymorphisms ◽  
Necrosis Factor Alpha ◽  
Single Nucleotide ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor

Introduction: Cytokines act as a mediator of inflammation in childhood nephrotic syndrome. Polymorphisms of cytokines genes may influence susceptibility to nephrotic syndrome (NS), as well as, patients’ steroid responses. Objective: To study the association of tumor necrosis factor-alpha single nucleotide polymorphisms (TNF-α SNP) (-863 C/A) with the development of NS in addition to access to their effects on serum level of TNF and the response to steroid therapy. Patients and Methods: This study included 60 patients (19 female and 41 male) with nephrotic syndrome; their ages ranged from 2 to 18 years. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to assess the TNF-α gene polymorphism. Results: According to the digestion pattern of RFLP-PCR products of TNF-α-863, this polymorphism had three genotypes, which were CC, CA, and AA, in both NS patients and controls. Also, the current result observed that -863 SNP do not affect the serum level of TNF-α and steroid responsiveness in patients with nephrotic syndrome. Conclusion: This polymorphism did not show any significant association with response to steroid therapy and TNF serum level neither at genotype nor at allele level.

scholarly journals Tumor necrosis factor (TNF)-α gene +489 polymorphisms: association with psoriatic arthritis

2013 ◽  
Vol 86 (1) ◽  
Author(s):  
G. Murdaca ◽  
F. Puppo
Keyword(s):  
Psoriatic Arthritis ◽  
Tumor Necrosis Factor ◽  
Single Nucleotide Polymorphisms ◽  
Tumor Necrosis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Tnf Α ◽  
The Difference ◽  
Necrosis Factor

Objective of this work was to investigate the role of single nucleotide polymorphisms (SNPs) at position +489 of the tumor necrosis factor (TNF)-α gene in generic susceptibility and severity of psoriatic arthritis (PsA). Fifty-seven Caucasian PsA patients diagnosed according to CASPAR criteria and 155 healthy matched controls were studied. PASI score, DAS28 and Disability INdex HAQ were calculated. Genomic DNA was extracted from peripheral blood samples and SNPs +489 G>A (rs 80267959) were amplified by PCR. The SNP +489 genotype was significantly associated with PsA susceptibility (p=0.0136) and severity of clinical and laboratory parameters (p values ranging from 0.016 to 2.908 x 10-12). The difference in severity was accounted for by the difference between the AA and GA genotypes with respect to the GG genotype. These findings suggest that TNF-α gene polymorphisms may influence PsA susceptibility and severity. Psoriatics arthritis (PsA) is a complex immunemediated disease that results from the interplay between multiple genetic and environmental factor [1]. Although the pathogenesis of PsA remains elusive, there is evidence that genetic factors may contribute to the etiology of the disease [2]. Is has been estimated that at least one third of the genetic contribution to PsA resides in the major histocompatibility complex (MHC) region [2]. The tumor necrosis factor (TNF)-α gene, which is located in the short arm of chromosome 6 in the MHC class III region between the HLA-B and HLA-DR genes, has been proposed as a major candidate gene in PsA [3]. This hypothesis is supported by studies which have found high serum, synovial fluid and synovial membrane TNF-α levels in patients with PsA [4,5]. Several single nucleotide polymorphisms (PNPs) have been identified in the TNF-α gene promoter [6]. In particular, two common polymorphisms, namely G to A substitutions at positions -238 and -308 have been studied in patients with PsA. However, association studies of these two TNF-α polymorphisms and genetic susceptibility to PsA have lead to conflicting results [7-12]. Previous studies have indicated the potential role of the SNP at +489 position in the first intron of the TNF-α gene in the susceptibility to some rheumatic autoimmune diseases like rheumatoid arthritis [13], systemic lupus erythematosus [14] and systemic sclerosis [15]. However, to our present knowledge, studies on the association of +489 polymorphism with PsA susceptibility and response to TNF-α inhibitors are not reported in the literature. Is this study we investigated the role of SNPs at +489 within the TNF-α gene in PsA susceptibility and severity.

scholarly journals Polymorphisms of Tumor Necrosis Factor Alpha in Moroccan Patients with Gastric Pathology: New Single-Nucleotide Polymorphisms in TNF-α−193(G/A)

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
A. Essadik ◽  
H. Jouhadi ◽  
T. Rhouda ◽  
S. Nadifiyine ◽  
A. Kettani ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
High Risk ◽  
Tumor Necrosis Factor ◽  
Promoter Region ◽  
Tumor Necrosis ◽  
Nucleotide Polymorphisms ◽  
Necrosis Factor Alpha ◽  
Single Nucleotide ◽  
Factor Alpha ◽  
Necrosis Factor

Polymorphisms in tumor necrosis factor alpha (TNF-α) gene are emerging as key determinants of gastric diseases. The TNF-α−308(G/A) and TNF-α−238(G/A) single-nucleotide polymorphisms SNPs are the most extensively studied. However, all these studies are conducted in Caucasian and Asian populations. Thus, for the first time in Africa, we sought to investigate whether polymorphisms in TNF-αgene were associated with the development of gastric pathology in Morocco. Two SNPs located in the promoter region (positions −308 and −238) in TNF-αgene were genotyped in 244 individuals (170 patients and 74 healthy controls). Odds ratios (ORs) and 95% confidence intervals (CI) were estimated using logistic regression analysis. The TNF-α−238(G/A) genotype was significantly associated with a high risk of gastritis and gastric cancer (GC) (P=0.001andP=0.002, resp.). Furthermore, a new polymorphism located in the promoter region at position −193 in TNF-αgene was identified. The distribution of this SNP was markedly different in patients suffering from ulcers. The association between TNF-α−193(G/A) genotype and high risk of ulcer was significant (P=0.03). These results suggest that the TNF-α−193(G/A) allele has a protective function against gastric cancer by developing ulcer.

scholarly journals Characterization of Single-Nucleotide Polymorphisms in the Tumor Necrosis Factor α Promoter Region and in Lymphotoxin α in Squamous Intraepithelial Lesions, Precursors of Cervical Cancer

2011 ◽  
Vol 4 (6) ◽  
pp. 336-344 ◽  
Author(s):  
Miriam Enriqueta Nieves-Ramirez ◽  
Oswaldo Partida-Rodriguez ◽  
Pedro Eduardo Alegre-Crespo ◽  
Maria del Carmen Tapia-Lugo ◽  
Martha Esthela Perez-Rodriguez
Keyword(s):  
Promoter Region ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Factor Α ◽  
Lymphotoxin Α ◽  
Intraepithelial Lesions ◽  
Necrosis Factor
Sign in / Sign up

Export Citation Format

Share Document