Faculty Opinions recommendation of Developmental regulation of a novel outwardly rectifying mechanosensitive anion channel in Caenorhabditis elegans.

2001 ◽  
Author(s):  
Ellen A Lumpkin
Keyword(s):  
Caenorhabditis Elegans ◽  
Developmental Regulation ◽  
Anion Channel

scholarly journals Selective lineage specification by mab-19 during Caenorhabditis elegans male peripheral sense organ development.

Genetics ◽  
1994 ◽  
Vol 138 (3) ◽  
pp. 675-688 ◽  
Author(s):  
M E Sutherlin ◽  
S W Emmons
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Physiological Stress ◽  
Developmental Regulation ◽  
Cell Lineage ◽  
Sense Organ ◽  
Sensory Ray ◽  
Hypodermal Cell ◽  
Physiological Stress Response ◽  
Male Specific

Abstract The action of the gene mab-19 is required for specification of a subset of Caenorhabditis elegans male peripheral sense organ (ray) lineages. Two mab-19 alleles, isolated in screens for ray developmental mutations, resulted in males that lacked the three most posterior rays. Cell lineage alterations of male-specific divisions of the most posterior lateral hypodermal (seam) blast cell, T, resulted in the ray loss phenotype in mab-19 mutant animals. Postembryonic seam lineage defects were limited to male-specific T descendent cell divisions. Embryonic lethality resulted when either mab-19 mutation was placed over a chromosomal deficiency encompassing the mab-19 locus. The earliest detectable defect was aberrant hypodermal cell movements during morphogenesis. From these data, it is inferred that both mab-19 alleles described are hypomorphs, and further reduction of mab-19 function results in embryos that are unable to complete morphogenesis. Thus, mab-19 may play a larger role in developmental regulation of hypodermal cell fate, including sensory ray development in males. Body morphology mutations, passage through the dauer stage, and heat or CdCl2 treatment suppressed mab-19 male phenotypes. A model is presented in which all three types of suppression result in a physiological stress response, which in turn leads to correction of the mab-19 defect.

Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation

2015 ◽  
Vol 40 (2) ◽  
pp. 257-268 ◽  
Author(s):  
Krishnan Gandhimathi ◽  
Sellamuthu Karthi ◽  
Paramasivam Manimaran ◽  
Perumal Varalakshmi ◽  
Balasubramaniem Ashokkumar
Keyword(s):  
Caenorhabditis Elegans ◽  
Developmental Regulation

Evolutionarily conserved WNK and Ste20 kinases are essential for acute volume recovery and survival after hypertonic shrinkage in Caenorhabditis elegans

2007 ◽  
Vol 293 (3) ◽  
pp. C915-C927 ◽  
Author(s):  
Keith P. Choe ◽  
Kevin Strange
Keyword(s):  
Caenorhabditis Elegans ◽  
Anion Channel ◽  
Transport Processes ◽  
Transepithelial Transport ◽  
C Elegans ◽  
Volume Recovery ◽  
Wnk Kinases ◽  
Wnk Kinase ◽  
Wnk Protein Kinases ◽  
First Time

Members of the germinal center kinase (GCK)-VI subfamily of Ste20 kinases regulate a Caenorhabditis elegans ClC anion channel and vertebrate SLC12 cation-Cl− cotransporters. With no lysine (K) (WNK) protein kinases interact with and activate the mammalian GCK-VI kinases proline-alanine-rich Ste20-related kinase (PASK) and oxidative stress-responsive 1 (OSR1). We demonstrate here for the first time that GCK-VI kinases play an essential role in whole animal osmoregulation. RNA interference (RNAi) knockdown of the single C. elegans GCK-VI kinase, GCK-3, dramatically inhibits systemic volume recovery and survival after hypertonic shrinkage. Tissue-specific RNAi suggests that GCK-3 functions primarily in the hypodermis and intestine to mediate volume recovery. The single C. elegans WNK kinase, WNK-1, binds to GCK-3, and wnk-1 knockdown gives rise to a phenotype qualitatively similar to that of gck-3(RNAi) worms. Knockdown of the two kinases together has no additive effect, suggesting that WNK-1 and GCK-3 function in a common pathway. We postulate that WNK-1 functions upstream of GCK-3 in a manner similar to that postulated for its mammalian homologs. Phylogenetic analysis of kinase functional domains suggests that the interaction between GCK-VI and WNK kinases first occurred in an early metazoan and therefore likely coincided with the need of multicellular animals to tightly regulate transepithelial transport processes that mediate systemic osmotic homeostasis.

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