Faculty Opinions recommendation of Engineered human IgG antibodies with longer serum half-lives in primates.

AbstractSt. Louis encephalitis virus (SLEV) is a mosquito-borne reemerging flavivirus in Argentina. It is currently necessary to develop specific serological tests that can efficiently discriminate the flaviviruses that circulate in our country. The immunoassays to diagnose SLEV lack specificity because they are based on the detection of structural viral proteins and the human immunoglobulins produced during infection against these proteins cross-react with other flaviviruses. Here, we describe an enzyme-immunoassay designed to detect human IgG antibodies specific to the viral nonstructural protein NS5. The results indicate that NS5 is a promising antigen useful to discriminate SLEV from other circulating flaviviruses.

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Recombinant Human IgG antibodies against Human Cytomegalovirus

Biomedical and Environmental Sciences ◽

10.1016/s0895-3988(08)60057-4 ◽

2008 ◽

Vol 21 (5) ◽

pp. 372-380 ◽

Cited By ~ 1

Author(s):

Tao DUAN ◽

Xiao-Fang WANG ◽

Shu-Yuan XIAO ◽

Shu-Yan GU ◽

Mi-Fang LIANG

Keyword(s):

Human Cytomegalovirus ◽

Igg Antibodies ◽

Human Igg

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Antigen-binding radioimmunoassays for human IgG antibodies to bovine β-lactoglobulin

Journal of Immunological Methods ◽

10.1016/0022-1759(83)90409-x ◽

1983 ◽

Vol 56 (2) ◽

pp. 175-183 ◽

Cited By ~ 4

Author(s):

M.W. Turner ◽

R. Paganelli ◽

R.J. Levinsky ◽

Antoinette Williams

Keyword(s):

Antigen Binding ◽

Igg Antibodies ◽

Human Igg ◽

Β Lactoglobulin

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Harnessing phage display technology for generating fully human IgG antibodies that neutralise elapid neurotoxins

Toxicon ◽

10.1016/j.toxicon.2018.10.068 ◽

2019 ◽

Vol 158 ◽

pp. S18

Author(s):

Aneesh Karatt-Vellatt ◽

Andreas H. Laustsen ◽

Edward W. Masters ◽

Saioa Oscoz ◽

Urska Pus ◽

...

Keyword(s):

Phage Display ◽

Igg Antibodies ◽

Human Igg ◽

Phage Display Technology ◽

Display Technology

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Antigen-binding affinity and thermostability of chimeric mouse-chicken IgY and mouse-human IgG antibodies with identical variable domains

Scientific Reports ◽

10.1038/s41598-019-55805-4 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Juho Choi ◽

Minjae Kim ◽

Joungmin Lee ◽

Youngsil Seo ◽

Yeonkyoung Ham ◽

...

Keyword(s):

Thermal Stability ◽

Binding Affinity ◽

Antigen Binding ◽

Chimeric Mouse ◽

Binding Parameters ◽

Igg Antibodies ◽

Human Igg ◽

Variable Domains ◽

Thermal Stability Of ◽

Parental Mouse

AbstractConstant (C)-region switching of heavy (H) and/or light (L) chains in antibodies (Abs) can affect their affinity and specificity, as demonstrated using mouse, human, and chimeric mouse-human (MH) Abs. However, the consequences of C-region switching between evolutionarily distinct mammalian and avian Abs remain unknown. To explore C-region switching in mouse-chicken (MC) Abs, we investigated antigen-binding parameters and thermal stability of chimeric MC-6C407 and MC-3D8 IgY Abs compared with parental mouse IgGs and chimeric MH Abs (MH-6C407 IgG and MH-3D8 IgG) bearing identical corresponding variable (V) regions. The two MC-IgYs exhibited differences in antigen-binding parameters and thermal stability from their parental mouse Abs. However, changes were similar to or less than those between chimeric MH Abs and their parental mouse Abs. The results demonstrate that mammalian and avian Abs share compatible V-C region interfaces, which may be conducive for the design and utilization of mammalian-avian chimeric Abs.

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Investigation of anti-Toxoplasma gondii antibodies in patients with neoplasia

Journal of Medical Microbiology ◽

10.1099/jmm.0.45587-0 ◽

2004 ◽

Vol 53 (12) ◽

pp. 1183-1186 ◽

Cited By ~ 33

Author(s):

Süleyman Yazar ◽

Ozan Yaman ◽

Bülent Eser ◽

Fevzi Altuntaş ◽

Fatih Kurnaz ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Patient Group ◽

Healthy Controls ◽

Igg Antibodies ◽

Human Igg ◽

Igm Antibodies ◽

Significant Difference

This study aimed to determine the prevalence of anti-Toxoplasma gondii antibodies in patients with neoplasia. One hundred and eight patients with neoplasia and 108 healthy controls were studied for the presence of anti-T. gondii antibodies using a micro ELISA and peroxidase-labelled anti-human IgG (rabbit) and IgM (goat). Anti-T. gondii IgG antibodies were detected in 68 (63.0 %) patients and in 21 (19.4 %) of the controls, which was a statistically significant difference. In addition, anti- T. gondii IgM antibodies were detected in seven (6.5 %) patients and in one (0.9 %) control. A high percentage of positivity for Toxoplasma antibodies in patients with neoplasia was detected. Therefore, parasitological surveys of this patient group should be periodically performed.

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