scholarly journals Specific diagnostic method for St. Louis Encephalitis Virus using a non-structural protein as antigen

2019 ◽  
Author(s):  
M.B. Simari ◽  
S.E. Goñi ◽  
V.C Luppo ◽  
C.M. Fabbri ◽  
M.H. Argüelles ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Diagnostic Method ◽  
Nonstructural Protein ◽  
Viral Proteins ◽  
Encephalitis Virus ◽  
Igg Antibodies ◽  
Structural Protein ◽  
Human Igg ◽  
Serological Tests ◽  
Human Immunoglobulins

AbstractSt. Louis encephalitis virus (SLEV) is a mosquito-borne reemerging flavivirus in Argentina. It is currently necessary to develop specific serological tests that can efficiently discriminate the flaviviruses that circulate in our country. The immunoassays to diagnose SLEV lack specificity because they are based on the detection of structural viral proteins and the human immunoglobulins produced during infection against these proteins cross-react with other flaviviruses. Here, we describe an enzyme-immunoassay designed to detect human IgG antibodies specific to the viral nonstructural protein NS5. The results indicate that NS5 is a promising antigen useful to discriminate SLEV from other circulating flaviviruses.

A Sandwich Enzyme Immunoassay for the Detection of Human IgG Antibodies to Dog Allergens

1987 ◽  
Vol 83 (1) ◽  
pp. 64-71 ◽  
Author(s):  
M. Viander ◽  
E. Nieminen ◽  
E. Valovirta ◽  
T. Vanto ◽  
L. Ingeman ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Igg Antibodies ◽  
Human Igg ◽  
Sandwich Enzyme Immunoassay

scholarly journals Specific diagnostic method for St. Louis encephalitis virus using a non-structural protein as the antigen

2020 ◽  
Vol 101 (2) ◽  
pp. 168-174
Author(s):  
Milagros Belén Simari ◽  
Sandra Elizabeth Goñi ◽  
Victoria Celina Luppo ◽  
Cintia Marcela Fabbri ◽  
Marcelo Horacio Argüelles ◽  
...  
Keyword(s):  
Diagnostic Method ◽  
Encephalitis Virus ◽  
Structural Protein

scholarly journals Epitope-Blocking Enzyme-Linked Immunosorbent Assay To Differentiate West Nile Virus from Japanese Encephalitis Virus Infections in Equine Sera

2007 ◽  
Vol 14 (8) ◽  
pp. 1024-1031 ◽  
Author(s):  
Yoko Kitai ◽  
Mizue Shoda ◽  
Takashi Kondo ◽  
Eiji Konishi
Keyword(s):  
West Nile Virus ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Immunosorbent Assay ◽  
Nonstructural Protein ◽  
Encephalitis Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Serological Tests ◽  
Nonstructural Protein 1

ABSTRACT West Nile virus (WNV) is now widely distributed worldwide, except in most areas of Asia where Japanese encephalitis virus (JEV) is distributed. Considering the movement and migration of reservoir birds, there is concern that WNV may be introduced in Asian countries. Although manuals and guidelines for serological tests have been created in Japan in preparedness for the introduction of WNV, differential diagnosis between WNV and JEV may be complicated by antigenic cross-reactivities between these flaviviruses. Here, we generated a monoclonal antibody specific for the nonstructural protein 1 (NS1) of WNV and established an epitope-blocking enzyme-linked immunosorbent assay that can differentiate WNV from JEV infections in horse sera. Under conditions well suited for our assay system, samples collected from 95 horses in Japan (regarded as negative for WNV antibodies), including those collected from horses naturally infected with JEV, showed a mean inhibition value of 8.2% and a standard deviation (SD) of 6.5%. However, inhibition values obtained with serum used as a positive control (obtained after 28 days from a horse experimentally infected with WNV) in nine separate experiments showed a mean of 54.4% and an SD of 7.1%. We tentatively determined 27.6% (mean + 3 × SD obtained with 95 negative samples) as the cutoff value to differentiate positive from negative samples. Under this criterion, two horses experimentally infected with WNV were diagnosed as positive at 12 and 14 days, respectively, after infection.

scholarly journals SARS-CoV-2 serological assay and viral testing: a report of professional football setting

2021 ◽  
pp. postgradmedj-2021-140176
Author(s):  
Bahar Hassanmirzaei ◽  
Zohreh Haratian ◽  
Ali Ahmadzadeh Amiri ◽  
Amir Ahmadzadeh Amiri ◽  
Navid Moghadam
Keyword(s):  
Standard Test ◽  
Football Players ◽  
Professional Football ◽  
Serological Testing ◽  
Igg Antibodies ◽  
Current Standard ◽  
Serological Tests ◽  
The Mean ◽  
Viral Testing ◽  
Pcr Test

Purpose of the studyPCR is the current standard test for the diagnosis of SARS-CoV-2 infection. However, due to its limitations, serological testing is considered an alternative method for detecting SARS-CoV-2 exposure. In this study, we measured the level of SARS-CoV-2 IgM and IgG antibodies of male professional football players and compared the results with the standard PCR test to investigate the association between the two tests.Study designParticipants were male professional football players and team officials. Nasopharyngeal swabs and peripheral blood samples were collected for the PCR and serological tests, respectively. Also, previous records of COVID-19 testing and symptoms were gathered. Those with previous positive PCR tests who tested negative for the second time were considered to be recovered patients.ResultsOf the 1243 subjects, 222 (17.9%) were seropositive, while 29 (2.3%) tested positive for the SARS-CoV-2 PCR test. Sixty percent of symptomatic cases with a negative PCR were found to be seropositive. The mean level of IgM was significantly higher in PCR-positive and symptomatic subjects, whereas the recovered cases showed significantly higher levels of IgG.ConclusionOur study revealed an inconsistency of results between the two tests; therefore, although application of serological assays alone seems insufficient in diagnosing COVID-19 disease, the findings are beneficial in the comprehension and the management of the disease.

scholarly journals A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Cécile Beck ◽  
Philippe Desprès ◽  
Sylvie Paulous ◽  
Jessica Vanhomwegen ◽  
Steeve Lowenski ◽  
...  
Keyword(s):  
High Performance ◽  
Neurological Diseases ◽  
Expression System ◽  
Cross Reactivity ◽  
Encephalitis Virus ◽  
Serological Tests ◽  
Multiplex Immunoassay ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Negative Controls

West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs). These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using theDrosophilaS2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.

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