Faculty Opinions recommendation of Functional analyses of tiptop and antennapedia in the embryonic development of Oncopeltus fasciatus suggests an evolutionary pathway from ground state to insect legs.

Aromatic esterase (A esterase, organophosphate-resistant esterase), aliesterase (B esterase, organophosphate-sensitive esterase), and acetylcholinesterase were localized in the developing embryo and in the young nymph of the large milkweed bug, Oncopeltus fasciatus (Dall.). The esterase complex varied qualitatively with embryonic development. Aromatic esterase occurred in the 3-day-old embryo, aromatic esterase and acetylcholine-esterase were found in the 4-day-old embryo, while all three esterases occurred in the 5-day-old embryo and in the young nymph. The distribution of each esterase remained fairly constant during embryonic development; aromatic esterase was located in many tissues and cells, aliesterase occurred in the pericardial cells, and acetylcholinesterase was found only in the neuropile of the nerve cord and brain. In the young nymph, the number of sites of aromatic esterase activity was reduced while additional sites of aliesterase occurred; acetylcholinesterase activity remained in the neuropile.

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Functional analyses in the hemipteran Oncopeltus fasciatus reveal conserved and derived aspects of appendage patterning in insects

Developmental Biology ◽

10.1016/j.ydbio.2004.04.005 ◽

2004 ◽

Vol 271 (2) ◽

pp. 306-321 ◽

Cited By ~ 83

Author(s):

David R Angelini ◽

Thomas C Kaufman

Keyword(s):

Oncopeltus Fasciatus ◽

Functional Analyses ◽

Appendage Patterning

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The States of Pluripotency: Pluripotent Lineage Development in the Embryo and in the Dish

ISRN Stem Cells ◽

10.1155/2014/208067 ◽

2014 ◽

Vol 2014 ◽

pp. 1-19 ◽

Cited By ~ 3

Author(s):

Joy Rathjen

Keyword(s):

Cell Differentiation ◽

Embryonic Development ◽

Ground State ◽

Inner Cell Mass ◽

Cell Mass ◽

Cell Lineage ◽

Precursor Cell ◽

Cell Populations ◽

Pluripotent Cell ◽

Intermediary Cell

The pluripotent cell lineage of the embryo comprises a series of temporally and functionally distinct intermediary cell states, the epiblast precursor cell of the newly formed blastocyst, the epiblast population of the inner cell mass, and the early and late epiblast of the postimplantation embryo, referred to here as early and late primitive ectoderm. Pluripotent cell populations representative of the embryonic populations can be formed in culture. Although multiple pluripotent cell states are now recognised, little is known about the signals and pathways that progress cells from the epiblast precursor cell to the late primitive ectoderm in the embryo or in culture. The characterisation of cell states is most advanced in mouse where conditions for culturing distinct pluripotent cell states are well established and embryonic material is accessible. This review will focus on the pluripotent cell states present during embryonic development in the mouse and what is known of the mechanisms that regulate the progression of the lineage from the epiblast precursor cell and the ground state of pluripotency to the late primitive ectoderm present immediately prior to cell differentiation.

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Diverging functions of Scr between embryonic and post-embryonic development in a hemimetabolous insect, Oncopeltus fasciatus

Developmental Biology ◽

10.1016/j.ydbio.2009.01.032 ◽

2009 ◽

Vol 329 (1) ◽

pp. 142-151 ◽

Cited By ~ 44

Author(s):

John Chesebro ◽

Steven Hrycaj ◽

Najmus Mahfooz ◽

Aleksandar Popadić

Keyword(s):

Embryonic Development ◽

Oncopeltus Fasciatus ◽

Hemimetabolous Insect

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Renilla Bioluminescence: A Morphologic Approach to the Location of Photocytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051050 ◽

1974 ◽

Vol 32 ◽

pp. 208-209

Author(s):

Ben O. Spurlock ◽

Milton J. Cormier

Keyword(s):

Excited State ◽

Ground State ◽

Molecular Basis ◽

Light Emission ◽

Chemical Basis ◽

Electronic Excited State ◽

Colonial Form ◽

The Common ◽

Eighteenth And Nineteenth Centuries ◽

Catalyzed Oxidation

The phenomenon of bioluminescence has fascinated layman and scientist alike for many centuries. During the eighteenth and nineteenth centuries a number of observations were reported on the physiology of bioluminescence in Renilla, the common sea pansy. More recently biochemists have directed their attention to the molecular basis of luminosity in this colonial form. These studies have centered primarily on defining the chemical basis for bioluminescence and its control. It is now established that bioluminescence in Renilla arises due to the luciferase-catalyzed oxidation of luciferin. This results in the creation of a product (oxyluciferin) in an electronic excited state. The transition of oxyluciferin from its excited state to the ground state leads to light emission.

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Live Confocal Analysis of Fertilization and Early Development

Microscopy and Microanalysis ◽

10.1017/s1431927600031135 ◽

2001 ◽

Vol 7 (S2) ◽

pp. 1012-1013

Author(s):

Uyen Tram ◽

William Sullivan

Keyword(s):

Drosophila Melanogaster ◽

Embryonic Development ◽

Real Time ◽

Early Development ◽

Fluorescent Probes ◽

Primary Defect ◽

Fluorescent Analysis ◽

Dynamic Event ◽

Enormous Amount ◽

Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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