Faculty Opinions recommendation of Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti.

2006 ◽  
Author(s):  
Vishvanath Nene
Keyword(s):  
Rna Interference ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Genetically Modified ◽  
Dengue Virus Type 2

scholarly journals Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti

2006 ◽  
Vol 103 (11) ◽  
pp. 4198-4203 ◽  
Author(s):  
A. W. E. Franz ◽  
I. Sanchez-Vargas ◽  
Z. N. Adelman ◽  
C. D. Blair ◽  
B. J. Beaty ◽  
...  
Keyword(s):  
Rna Interference ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Genetically Modified ◽  
Dengue Virus Type 2

scholarly journals Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito's RNA Interference Pathway

2009 ◽  
Vol 5 (2) ◽  
pp. e1000299 ◽  
Author(s):  
Irma Sánchez-Vargas ◽  
Jaclyn C. Scott ◽  
B. Katherine Poole-Smith ◽  
Alexander W. E. Franz ◽  
Valérie Barbosa-Solomieu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Type 2

Variation Among Strains of Aedes aegypti in Susceptibility to Oral Infection with Dengue Virus Type 2

1990 ◽  
Vol 43 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Isabelle Tardieux ◽  
Laurent Lapchin ◽  
François Rodhain ◽  
Olivier Poupel
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Oral Infection ◽  
Dengue Virus Type 2

scholarly journals Demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of Aedes aegypti

2018 ◽  
Vol 12 (8) ◽  
pp. e0006754 ◽  
Author(s):  
Irma Sánchez-Vargas ◽  
Laura C. Harrington ◽  
Jeffrey B. Doty ◽  
William C. Black ◽  
Ken E. Olson
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Laboratory Strain ◽  
Venereal Transmission ◽  
Dengue Virus Type 2

scholarly journals Comparison of Dengue Virus Type 2-Specific Small RNAs from RNA Interference-Competent and –Incompetent Mosquito Cells

2010 ◽  
Vol 4 (10) ◽  
pp. e848 ◽  
Author(s):  
Jaclyn C. Scott ◽  
Doug E. Brackney ◽  
Corey L. Campbell ◽  
Virginie Bondu-Hawkins ◽  
Brian Hjelle ◽  
...  
Keyword(s):  
Rna Interference ◽  
Dengue Virus ◽  
Virus Type ◽  
Small Rnas ◽  
Mosquito Cells ◽  
Dengue Virus Type 2

scholarly journals Domain-III FG loop of the dengue virus type 2 envelope protein is important for infection of mammalian cells and Aedes aegypti mosquitoes

Virology ◽  
2010 ◽  
Vol 406 (2) ◽  
pp. 328-335 ◽  
Author(s):  
Steven M. Erb ◽  
Siritorn Butrapet ◽  
Kelley J. Moss ◽  
Betty E. Luy ◽  
Thomas Childers ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Envelope Protein ◽  
Mammalian Cells ◽  
Domain Iii ◽  
Dengue Virus Type 2

scholarly journals RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol.

2018 ◽  
Vol 3 (1) ◽  
pp. 52
Author(s):  
Yovilianda Maulitiva Untoro ◽  
Teguh Hari Sucipto ◽  
Harsasi Setyawati ◽  
Siti Churrotin ◽  
Ilham Harlan Amarullah ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Rna Isolation ◽  
Guanidinium Thiocyanate ◽  
The World ◽  
Dengue Virus Type 2

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as  the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol  can used as precipitation solvent for isolating RNA.

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